CAZyme Information: EPrPIT00000016849-p1

Basic Information

• Species: Globisporangium irregulare
• Lineage: Oomycota; NA; ; Pythiaceae; Globisporangium; Globisporangium irregulare
• CAZyme ID: EPrPIT00000016849-p1
• CAZy Family: CBM47
• CAZyme Description: Endo-1,3(4)-beta-glucanase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
721		80074.84	6.9459

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_GirregulareDAOMBR486	13869	1223557	65	13804

• Gene Location: location=PirrDAOMBR486_SC0184:34684-36934(+)

Full Sequence      

MLCLSFTQVTALAASLLLAATASLLPGASAQDTWETGSLVPNATLVEHTTAMTSLCAPRN
VDVIPGRPIPTNSWWGNLVTCDPTSNVTQPIWPNPFALSVDTIGTNGLFGFAISYPYRMR
FFGDYQANGVPNYYAHAVLKEVVFSSAEFASTTPTFRVASWTDLGVKLQMRIGAASTATQ
ILETNVVSGMPYFTTKYQSLTPRVALDNPILTINGAGYAXXXXXXXXXXXXGKRFLINTV
NGNRWILYAVGGATFTFRLESNMLLRSTSIFNGVLRMVNTLNATHTTSYDFYRECIATGG
SLNVTTDSAYAFQWETSGDCLMGLMHYALPHQRASMDTTSAVRILGVSLNSTTRGEMSAF
ATRTQPPTWKLVETSTIPITYYPKTRWTNTSAVGSGILQTLIDDVQATWSIPTQGSFYFN
GKAAQKYASLCLMANDRVIVGNDVSILQRCVTKLEVLIEPFLDNAWTYKLKYDKVYGGVV
SSEGFVSNDMNADFGNTVYNDHHFHYGYWVYTSAVLNFLHPTWIRNKDLNTMARLLLRDV
ANPSSNDPYFPKFRSFDWFRGHSYSHGVTGFGDGKDQESTSEDVNFSYALYLYALSTDNA
RMTAVGKLMVKLNARSIQTYFLMESTNTVQPAAILPHKAPGIFFDNKVDYATWFSAEKYC
IHGIQMIPVTPVTEFVRTKTFVKEEWDSVLSKEVIVTTNDTCQVDDGLSRSWALYMAAQL
N

Enzyme Prediction     

No EC number prediction in EPrPIT00000016849-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	66	693	5.2e-165	0.8906752411575563

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
407548	Glyco_hydro81C	3.44e-146	397	714	10	349	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
227785	Acf2	2.23e-134	68	720	89	755	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
397619	Glyco_hydro_81	2.38e-41	66	380	12	313	Glycosyl hydrolase family 81. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein ENGL1, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCA25009.1|GH81	1.67e-238	32	718	64	786
UIZ20868.1|GH81	3.39e-231	38	718	87	790
UIZ20872.1|GH81	3.39e-231	38	718	87	790
UIZ20875.1|GH81	3.39e-231	38	718	87	790
UIZ20873.1|GH81	9.62e-231	38	718	87	790

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4K3A_A	8.18e-91	58	718	30	716	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K3A_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
5XBZ_A	1.37e-90	58	718	55	741	Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XBZ_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XC2_A Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_C Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_D Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei]
4K35_A	6.25e-87	58	718	30	716	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K35_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
6FOP_A	4.42e-25	398	690	320	603	Chain A, Glycoside hydrolase family 81 [Acetivibrio thermocellus ATCC 27405]
5V1W_A	4.12e-16	407	668	350	604	Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09850|ENG2_SCHPO	2.38e-94	68	718	34	700	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1
sp|Q12168|ENG2_YEAST	1.95e-79	60	700	102	730	Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1
sp|D4AZ24|ENG1_ARTBC	2.19e-77	65	691	222	842	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
sp|P53753|ENG1_YEAST	5.80e-76	68	720	433	1112	Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1
sp|Q9UT45|ENG1_SCHPO	3.51e-74	233	720	225	737	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000310	0.999683	CS pos: 30-31. Pr: 0.9678

TMHMM  Annotations     

There is no transmembrane helices in EPrPIT00000016849-p1.