CAZyme Information: EPrPIT00000015671-p1

Basic Information

• Species: Globisporangium irregulare
• Lineage: Oomycota; NA; ; Pythiaceae; Globisporangium; Globisporangium irregulare
• CAZyme ID: EPrPIT00000015671-p1
• CAZy Family: AA17
• CAZyme Description: Carbohydrate esterase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
454	PirrDAOMBR486_SC0109|CGC1	50167.29	7.6450

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_GirregulareDAOMBR486	13869	1223557	65	13804

• Gene Location: location=PirrDAOMBR486_SC0109:40930-42804(+)

Full Sequence      

MAIPTSAAHQTESLMPASRFPTYPMADADAGVVPAPPSSLSSENALVHNFDLSKYKDGRY
KLVPASARDQIRYTGRFLHHGPFAKRKSKVNTVQFDWPGVAFEIVVRGTNTVAIRLKGDG
NYFNIFIDGAFRCILKASLNATCCEVATDLDPKSEYTVTITKRTEPQMRGALSTFKVCTF
YGFIVEASADVLPCKPAPVRKIEFIGDNVTCAFGNEGKVTSSKNRFGMKGRMENVHNGYA
CILARMFNAEAHVLAWSGKGVHSNSADWGPNMPALWKNTIASREGLWDMSSWTPDVVVVN
LGANDLFPPASLETEIVGAYALFLAEVRQYRPDAHIFCVAYDDGCMSAEDSEINRSHVSL
QLQEIVKVAMSKVNKHDSRMHYAFIKIDGGLEESDYATLMHYAVNGHLKIANLLAEEIAL
KAKWRVESRPSTMPYPQEKSQILMPKDSSNCTIS

Enzyme Prediction     

No EC number prediction in EPrPIT00000015671-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CE2	201	418	5.6e-54	0.9952153110047847

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
238869	Endoglucanase_E_like	3.72e-30	201	418	1	168	Endoglucanase E-like members of the SGNH hydrolase family; Endoglucanase E catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
407839	CE2_N	6.61e-17	73	193	1	106	Carbohydrate esterase 2 N-terminal. This is the N-terminal beta-sheet domain with jelly roll topology found in CE2 acetyl-esterase from the bacterium Clostridium thermocellum. This enzyme displays dual activities, it catalyses the deacetylation of plant polysaccharides and also potentiates the activity of its appended cellulase catalytic module through its noncatalytic cellulose binding function. This N-terminal jelly-roll domain appears to extend the substrate/cellulose binding cleft of the catalytic domain in C.thermocellum.
238141	SGNH_hydrolase	2.57e-06	202	416	1	186	SGNH_hydrolase, or GDSL_hydrolase, is a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the typical Ser-His-Asp(Glu) triad from other serine hydrolases, but may lack the carboxlic acid.
404371	Lipase_GDSL_2	5.17e-06	283	407	51	174	GDSL-like Lipase/Acylhydrolase family. This family of presumed lipases and related enzymes are similar to pfam00657.
238871	XynB_like	0.001	294	337	41	83	SGNH_hydrolase subfamily, similar to Ruminococcus flavefaciens XynB. Most likely a secreted hydrolase with xylanase activity. SGNH hydrolases are a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ26481.1|CE2	1.11e-212	47	454	77	522
CCA14218.1|CE2	9.90e-136	49	448	49	460
ADO75380.1|CE2	5.81e-43	71	425	46	369
AEY66733.1|CE2	3.59e-36	94	424	127	436
ACL76504.1|CE2	4.72e-35	94	424	127	436

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2WAO_A	1.78e-33	71	424	11	333	Chain A, ENDOGLUCANASE E [Acetivibrio thermocellus]
2WAB_A	4.69e-33	71	424	11	333	Chain A, ENDOGLUCANASE E [Acetivibrio thermocellus]
4XVH_A	1.25e-21	92	424	12	329	Crystal structure of a Corynascus thermopiles (Myceliophthora fergusii) carbohydrate esterase family 2 (CE2) enzyme plus carbohydrate binding domain (CBD) [Chaetomium]
2W9X_A	2.66e-13	78	305	30	248	The active site of a carbohydrate esterase displays divergent catalytic and non-catalytic binding functions [Cellvibrio japonicus],2W9X_B The active site of a carbohydrate esterase displays divergent catalytic and non-catalytic binding functions [Cellvibrio japonicus]
3U37_A	1.29e-08	59	424	37	406	An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_B An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_C An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_D An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_E An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_F An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_G An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316],3U37_H An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus. [Butyrivibrio proteoclasticus B316]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P10477|CELE_ACET2	3.42e-31	63	424	484	814	Cellulase/esterase CelE OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celE PE=1 SV=2
sp|B3PDE5|CE2B_CELJU	1.32e-12	78	305	30	248	Acetylxylan esterase / glucomannan deacetylase OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=ce2C PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000069	0.000001

TMHMM  Annotations     

There is no transmembrane helices in EPrPIT00000015671-p1.