CAZyme Information: EPrPIT00000014004-p1

Basic Information

• Species: Globisporangium irregulare
• Lineage: Oomycota; NA; ; Pythiaceae; Globisporangium; Globisporangium irregulare
• CAZyme ID: EPrPIT00000014004-p1
• CAZy Family: AA17
• CAZyme Description: Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit STT3A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
897		99050.78	7.4910

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_GirregulareDAOMBR486	13869	1223557	65	13804

• Gene Location: location=PirrDAOMBR486_SC0028:70499-73308(+)

Full Sequence      

MAPASKTKAKKKLADPAVEAHAPSATHDTPVHAPVSSKNEPAAPAASNLVFGVSLDSPHD
IEDESSVLAPIAKYAILGMICLMSFAIRLFAVVRYESVIHEFDPYFNFRTTKYLASEGFL
EFLDWFDDRAWYPLGRIIGGTIYPGLMYTAAMVYWVLNLLNISINVRNTCVFLAPIFAAN
TAIASYLLTKEVTKRSSAGLMAAAFAGIVPSYISRSVGGSYDNEGVAIFALIFTFYLWIK
AVHTGSMFWSASCALSYFYMVAAWGGYVFIINMIPIYVLVMIFAGRYSPRLYIAYSTFYT
LGSLMAMQVPFVGFNVIKQAECAGSHGVFVLLQVFCFVNWLRNYISAASFRRLVLFGAGA
IVVGFASILLVLQLLGKVQWTGRSLTLLDPTYASKYIPIIASVSEHQPTTWTSYFFDLHV
LVPFAPVGLYFLFSNITDGGIFVILYGTVAWYFAGVMVRLMLTLAPIACILGAIGISSTL
RKFMGILKRPADFVVQKNGAQEIPVPVSLSIIGGLLILLLSYTFHATYVSSMAYSSPSIV
IEAGRTQSGERVNFDDYREAYFWLRQNTEADAKIMAWWDYGYQMSAMANRTVIVDNNTWN
NTHIATVGRALASTEEEAYPIMQSLDVDYVLVIFGGLTGYSSDDINKFLWPVRIGSGVFP
NDMPSERDFYSASGNFDVGAGGSKTLHNCLAYKLCYYRFGEVMTDYQRPPGYDRARNTEV
GVKNIKLTHLEESFTSEHWIVRIFKVKKQPNVEPTTALKKARLSEASAAGKDVSQEKTHF
LGCVTGEDMLGNDRVYSGGSSGANYNLALHHAKNKNKRYFALSRVGAEGHAFAFNKLPVN
AGDFDGNDAGCTRPCMDSQEHFCGCADGGCSDQNVAPGKGQEHNRRWAIYERDSVDN

Enzyme Prediction     

EC	2.4.99.18:21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT66	73	707	3.3e-170	0.86002886002886

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396873	STT3	6.19e-112	73	535	3	478	Oligosaccharyl transferase STT3 subunit. This family consists of the oligosaccharyl transferase STT3 subunit and related proteins. The STT3 subunit is part of the oligosaccharyl transferase (OTase) complex of proteins and is required for its activity. In eukaryotes, OTase transfers a lipid-linked core-oligosaccharide to selected asparagine residues in the ER. In the archaea STT3 occurs alone, rather than in an OTase complex, and is required for N-glycosylation of asparagines.
224206	Stt3	2.09e-67	70	748	15	731	Asparagine N-glycosylation enzyme, membrane subunit Stt3 [Posttranslational modification, protein turnover, chaperones].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ23018.1|GT66	0.0	27	892	20	882
CCA15036.1|GT66	0.0	62	890	51	877
CCA23157.1|GT66	0.0	62	890	51	877
AFJ69334.1|GT66	0.0	62	891	53	890
ACI33169.1|GT66	3.47e-280	73	751	19	699

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6FTG_5	1.11e-279	73	751	19	699	Subtomogram average of OST-containing ribosome-translocon complexes from canine rough microsomal membranes [Canis lupus familiaris],6FTI_5 Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Programmed 80S Ribosome [Canis lupus familiaris]
6FTJ_5	1.62e-279	73	747	19	695	Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Non-programmed 80S Ribosome [Canis lupus familiaris]
6S7O_A	3.16e-279	73	751	19	699	Cryo-EM structure of human oligosaccharyltransferase complex OST-A [Homo sapiens]
6S7T_A	2.70e-262	73	778	73	802	Cryo-EM structure of human oligosaccharyltransferase complex OST-B [Homo sapiens]
6C26_A	5.93e-216	66	748	10	684	The Cryo-EM structure of a eukaryotic oligosaccharyl transferase complex [Saccharomyces cerevisiae S288C],6EZN_F Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex [Saccharomyces cerevisiae S288C],7OCI_F Chain F, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 [Saccharomyces cerevisiae S288C]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|F1PJP5|STT3A_CANLF	2.85e-279	73	751	19	699	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Canis lupus familiaris OX=9615 GN=STT3A PE=1 SV=3
sp|P46978|STT3A_MOUSE	4.03e-279	73	751	19	699	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Mus musculus OX=10090 GN=Stt3a PE=1 SV=1
sp|P46977|STT3A_HUMAN	1.62e-278	73	751	19	699	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Homo sapiens OX=9606 GN=STT3A PE=1 SV=2
sp|Q5RCE2|STT3A_PONAB	1.62e-278	73	751	19	699	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Pongo abelii OX=9601 GN=STT3A PE=2 SV=1
sp|Q2KJI2|STT3A_BOVIN	2.30e-278	73	751	19	699	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A OS=Bos taurus OX=9913 GN=STT3A PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000057	0.000000

TMHMM  Annotations     

Start	End
74	93
135	157
170	189
199	218
225	247
262	284
291	313
323	341
353	375
411	433
440	457
461	480
507	529