CAZyme Information: EPrPAT00000021794-p1

Basic Information

• Species: Pythium aphanidermatum
• Lineage: Oomycota; NA; ; Pythiaceae; Pythium; Pythium aphanidermatum
• CAZyme ID: EPrPAT00000021794-p1
• CAZy Family: GT2
• CAZyme Description: Chitin synthase (Chitin-UDP-GlcNac-transferase).

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
442	PaphDAOMBR444_SC0500|CGC1	50386.49	4.8961

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PaphanidermatumDAOMBR444	12363	1223555	58	12305

• Gene Location: location=PaphDAOMBR444_SC0500:21748-23720(-)

Full Sequence      

MDPEDDYSLSKAYSGENVWQNVVICIVADGREQINPKMLEYLDSVGLYDEDLLTINSAGI
GAQCHMFERTIQLTMNGKHLLPMQTIFVLKENKASKLDSHNWYFNAFAEQIQPEYTILMD
VGTMLTKSALYHMLYAFETNPQIGGACGQLTVEDPFRNLSNWVISAQHFEYKIANILDKS
LESCFGFISVLPGAFSGYRYKAIRGAPLDAYFQTLNYVLDMSVYMYNVVYLFGLLMLVII
GLGNNPKHMKLTYYFVSSVFADLSWGTKGLEEEPTLSPGEDNEKGDFKDVLAKRKALEER
RREFTLRQDNKKKQFEAFRTNVLLTWAFSNLIFALAIIYFSSANTYLPALYLVVAVINSI
RLLGCVSHLIYTWTEDFRSLVLNTDKGGDDKDGCRGPDDSYCRLDDHYAQLIEDQRVFVS
ERNMTGAPQSEPLSFYSLRSQI

Enzyme Prediction     

EC	2.4.1.16:26

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	12	137	2.8e-43	0.8159509202453987

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396286	Chitin_synth_1	1.05e-49	15	137	33	163	Chitin synthase. This region is found commonly in chitin synthases classes I, II and III. Chitin a linear homopolymer of GlcNAc residues, it is an important component of the cell wall of fungi and is synthesized on the cytoplasmic surface of the cell membrane by membrane bound chitin synthases.
133033	Chitin_synth_C	1.91e-37	93	221	51	179	C-terminal domain of Chitin Synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin. Chitin synthase, also called UDP-N-acetyl-D-glucosamine:chitin 4-beta-N-acetylglucosaminyltransferase, catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of GlcNAc residues formed by covalent beta-1,4 linkages. Chitin is an important component of the cell wall of fungi and bacteria and it is synthesized on the cytoplasmic surface of the cell membrane by membrane bound chitin synthases. Studies with fungi have revealed that most of them contain more than one chitin synthase gene. At least five subclasses of chitin synthases have been identified.
133045	CESA_like	2.63e-18	86	204	56	167	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.
367353	Chitin_synth_2	1.05e-12	114	206	203	292	Chitin synthase. Members of this family are fungal chitin synthase EC:2.4.1.16 enzymes. They catalyze chitin synthesis as follows: UDP-N-acetyl-D-glucosamine + {(1,4)-(N-acetyl-beta-D-glucosaminyl)}(N) <=> UDP + {(1,4)-(N-acetyl-beta-D-glucosaminyl)}(N+1).
224136	BcsA	1.61e-08	86	204	115	227	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEZ51041.1|GT2	8.36e-283	1	442	245	901
AGT99185.1|GT2	2.58e-176	4	422	266	898
ARC77249.1|GT2	1.03e-175	3	422	265	898
ARC77250.1|GT2	1.03e-175	3	422	265	898
ARC77245.1|GT2	1.45e-175	4	422	266	898

PDB Hits     

EPrPAT00000021794-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P48017|CHS_SAPMO	1.15e-55	15	216	276	473	Chitin synthase OS=Saprolegnia monoica OX=37553 GN=CHS PE=3 SV=1
sp|J9VWT0|CHS6_CRYNH	3.99e-50	15	212	374	587	Chitin synthase 6 OS=Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) OX=235443 GN=CHS6 PE=2 SV=2
sp|P30599|CHS2_USTMA	9.55e-50	15	213	432	637	Chitin synthase 2 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CHS2 PE=3 SV=2
sp|J9VMX7|CHS7_CRYNH	1.31e-49	12	213	248	462	Chitin synthase 7 OS=Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) OX=235443 GN=CHS7 PE=2 SV=2
sp|J9VGT6|CHS8_CRYNH	1.66e-49	8	212	406	624	Chitin synthase 8 OS=Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) OX=235443 GN=CHS8 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000069	0.000000

TMHMM  Annotations     

Start	End
222	244
317	339
349	371