CAZyme Information: EPrPAT00000019015-p1

Basic Information

• Species: Pythium aphanidermatum
• Lineage: Oomycota; NA; ; Pythiaceae; Pythium; Pythium aphanidermatum
• CAZyme ID: EPrPAT00000019015-p1
• CAZy Family: GH47
• CAZyme Description: UDP-glucose:glycoprotein glucosyltransferase.

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1599		180051.19	5.9396

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PaphanidermatumDAOMBR444	12363	1223555	58	12305

• Gene Location: location=PaphDAOMBR444_SC0236:8073-13085(+)

Full Sequence      

MVAPSGRRVLRLLGASAALVCAALTAQVEARGVHVNLTATWPSSPLYPLLEASEFLAEEN
PLYFWPFAVDKAQEIAPHTKNMLELTISTRTYSAKVEMFRQLALDSSVRVCGPDAEAWAV
VYKDKHCVTAVACSPSELSTALSSLHETTNKVCFAAGVNDVELSADHKFPHTMPPRDGSV
ILYGSLGSASLYEFHRELRVLAKDGAIAYMLRHYPKDSTLETLLQGYGVALDIKNMEYKT
IDDSKKAGEAEDATGDEEEEEEEMRDDEDDDEEVGADEEIDGLVFSNLFKEFASIKSELK
VFRDHLVKQHDQAEGEIKAWHLKDIGISAAREILDSKNPLKRLRILAQDFPKHAKKLAFS
RKALSDELREEIDSRRNEAMMAGLLNRFLVNGIVVDPMQRSFNIFDFVSTLKKEWSIAKR
IASLPLTAEEREHMLTSIRESKSEKGAVRIRVRGPIDGFAPLYLNNIETDHQTKQWPTQV
ANLRRPSWNLIFIRKNMYEYVVAFDPTSQQGRIALGQISFLRMRGAPLQWGFLISTKELM
AAKTKDERDEIVKQYKQAEFETKATGWHFAKLLLMTKEKDTPDQDGMTGRIVQGFIEGVA
DEGGEVTVQTLIDCYLDTVGGSFSRGKSRSEALATMRSDKFDEEVISMTEFIGHKHLPFE
SALFNGVLKTDLDLQNDIMNHFGRDQALFQAMARDGELNGEMDLVEELLSAQEAYPSYFS
LFSEGASTEDENVASIPTANHLFSNDENAALENLVSKLVKFAHQANTATQPKKQTLLFWA
DLNDPVHAGHVRRAYKAVQENLGKVMRVGVIHRLKSAKELGVGELVASIVDVIGDSDAES
HVRLVTEALACVTKGKTVDATKSKLGGIIEKFTKWKSDKTLVYIKDKILVDGDVPISDEL
HVSLVQLNRALALTQSRSTSGVDSAQLYLNGYRIDLPEHPITEEDIAALIGYDMKHRSQP
VAKAFIKRSAMYTAAEANERSMEIVKASAVVDEYRKTERHSGMNLTDSNIVIRLPGDESL
RVVAYIDPLTEAAQRMSSLLVMLRAQLNATVELLPLPASDYSEFPLQRFYRYLFDKKTTR
ASSVTFRKLPIKPILTMKIDTPEAWNVQIHTTADDLDNLRVDPDSPADVRAIKTASFRLE
SLLVYGQCRDKTFDQYIPPNGLQLVLERDIGSQRLHRDTLVMKNFGYFQLQATPGVWNLH
LARGRAVDLFEIVEPGTDKRLQSTDIIVSGFTSRLEQLLVQKRPGKEHDELLAKTDTDKT
SSGSDVEPIAADAGILGSLKSMFSMFGKKKVVAKENESPANDKAVATVDGANTGRKGETI
HVFSLASGHLYERFLKIMMLSVLKRTENPVTFWLLENFLSPDFKSSIPALRSKFGMDIRL
VTYKWPNWLRQQTQKQRIIWGYKILFLDVLFPLGVQKIIYVDADQVVRADLKELWEMDLH
GAPYGYTPFCDSRNVGFQFWRQGYWKDHLRGRPYHISALYVVDLALFRQMAAGDMLRAIY
DQLSADPNSLSNLDQDLPNYAQHQIPIHSLPNEWLWCESWCSDESKERAKTIDLCNNPKH
KEPKLDMAKRVISGELFNESWLELDQEVKDAEKVFAITV

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1320	1565	4.6e-124	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1320	1590	1	267	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	1.90e-165	1320	1565	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
132996	Glyco_transf_8	3.44e-59	1320	1560	1	243	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
408199	Thioredoxin_12	7.57e-35	43	218	1	181	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	2.77e-25	1146	1253	2	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ20865.1|GT24	0.0	8	1592	2	1657
CCA14500.1|GT24	0.0	27	1593	32	1663
AIG55512.1|GT24	0.0	23	1597	16	1517
AIG56125.1|GT24	0.0	15	1595	10	1505
AHH42333.1|GT24	3.96e-209	29	1589	20	1490

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	3.79e-146	25	1590	2	1446	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TRT_A	7.16e-146	25	1590	2	1446	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	4.83e-145	25	1590	2	1446	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	2.12e-135	1023	1590	659	1212	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	1.37e-120	33	1527	4	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q6P5E4|UGGG1_MOUSE	5.51e-204	18	1595	32	1527	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9JLA3|UGGG1_RAT	1.09e-202	18	1595	32	1527	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	1.30e-197	27	1592	41	1524	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q9NYU1|UGGG2_HUMAN	1.11e-193	1	1589	1	1496	UDP-glucose:glycoprotein glucosyltransferase 2 OS=Homo sapiens OX=9606 GN=UGGT2 PE=1 SV=4
sp|Q09332|UGGG_DROME	2.30e-188	37	1589	34	1505	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.001035	0.998921	CS pos: 30-31. Pr: 0.9492

TMHMM  Annotations     

There is no transmembrane helices in EPrPAT00000019015-p1.