CAZyme Information: EPrPAT00000014678-p1

Basic Information

• Species: Pythium aphanidermatum
• Lineage: Oomycota; NA; ; Pythiaceae; Pythium; Pythium aphanidermatum
• CAZyme ID: EPrPAT00000014678-p1
• CAZy Family: AA17
• CAZyme Description: ER degradation-enhancing alpha-mannosidase.

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
740	PaphDAOMBR444_SC0035|CGC1	82461.21	6.5714

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PaphanidermatumDAOMBR444	12363	1223555	58	12305

• Gene Location: location=PaphDAOMBR444_SC0035:34401-36788(+)

Full Sequence      

MPPLRLVATLLLLCLSVVSRVAVARMHASEIRHLRQLARDMFYHGYHSYMEHAFPWDELK
PLSCEGRRWDRRERGDLDDVLGGYALTLVDSLDMLAVLGDRDEFKRAVGLVVDTVHFDRD
VTVSVFESTIRVIGGLLSAHLLASPDHHGVLKAEEYSGQLLDLAVDLGRRLLPAFDTPTG
IPVHRVHLQRGMLKDAPAITCPAAAGSLLVELSYLSRLSGIEAFEDRARNAVVALWLRRS
DLNLLGSSIDVFSGEWQTSHVGIGAGLDSFFEYLLKYHLVSGNSAWLEMFNISYSAVETH
MNHDDFHIEVDMYHGRQAIRSRRVSALQAFWPGLQVLAGDVSRAMRSHDKLFLLWNKFGA
MPEIFDLSGTESGGIISWARTSPLRPELIESTYHLYQATRDHKYLKIGRKLLEDIERVSR
VECGYAAVGNIHTLAVEDRMDSYFLSETVKYLYLLFSEDPDVIVPPMALPAASNITNQST
PSNASMYSRALKSSDVVFSTEGHVLPLDATLFTRDSDRSSLQETLECENGHALVQRERRR
QHSGATDAAASLSFVPIGVSIRVGNSHVSTMVASPATFGHQFGRFGHVEAPLLLYNRDIG
DACEPLADHYDDIVGRIVMVTRGRCSFAEKALHIQQAGGAGVIVINKKSKSKTQPQGHFV
INEGHVYKEINEEKMHLLPEKRIACSHIFIEDSEVHAYLVALSKCSIIIVCNKVDNLVDH
ELSALLLKHNLVGLVIDQHL

Enzyme Prediction     

EC	3.2.1.113:20

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH47	41	506	5.7e-132	0.9955156950672646

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396217	Glyco_hydro_47	2.42e-137	41	507	1	453	Glycosyl hydrolase family 47. Members of this family are alpha-mannosidases that catalyze the hydrolysis of the terminal 1,2-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2).
240427	PTZ00470	3.55e-72	35	465	73	503	glycoside hydrolase family 47 protein; Provisional
239041	PA_EDEM3_like	5.22e-12	573	655	2	83	PA_EDEM3_like: protease associated domain (PA) domain-containing EDEM3-like proteins. This group contains various PA domain-containing proteins similar to mouse EDEM3 (ER-degradation-enhancing mannosidase-like 3 protein). EDEM3 contains a region, similar to Class I alpha-mannosidases (gylcosyl hydrolase family 47), N-terminal to the PA domain. EDEM3 accelerates glycoprotein ERAD (ER-associated degradation). In transfected mammalian cells, overexpression of EDEM3 enhances the mannose trimming from the N-glycans, of a model misfolded protein [alpha1-antitrypsin null (Hong Kong)] as well as, from total glycoproteins. Mannose trimming appears to be involved in the selection of ERAD substrates. EDEM3 has a different specificity of trimming than ER alpha-mannosidase 1. The significance of the PA domain to EDEM3 has not been ascertained. It may be a protein-protein interaction domain. At peptidase active sites, the PA domain may participate in substrate binding and/or promoting conformational changes, which influence the stability and accessibility of the site to substrate.
240122	PA_subtilisin_1	1.05e-09	575	646	2	72	PA_subtilisin_1: Protease-associated domain containing subtilisin-like proteases, subgroup 1. A subgroup of PA domain-containing subtilisin-like proteases. The significance of the PA domain to many of the proteins in which it is inserted is undetermined. It may be a protein-protein interaction domain. At peptidase active sites, the PA domain may participate in substrate binding and/or promoting conformational changes, which influence the stability and accessibility of the site to substrate. Proteins into which the PA domain is inserted include the following subtilisin-like proteases: i) melon cucumisin, ii) Arabidopsis thaliana Ara12, iii) Alnus glutinosa ag12, iv) members of the tomato P69 family, and v) tomato LeSBT2. However, these proteins belong to other subtilisin-like subgroups. Relatively little is known about proteins in this subgroup.
238300	PA	2.57e-09	577	646	1	78	PA: Protease-associated (PA) domain. The PA domain is an insert domain in a diverse fraction of proteases. The significance of the PA domain to many of the proteins in which it is inserted is undetermined. It may be a protein-protein interaction domain. At peptidase active sites, the PA domain may participate in substrate binding and/or promoting conformational changes, which influence the stability and accessibility of the site to substrate. Proteins into which the PA domain is inserted include the following: i) various signal peptide peptidases including, hSPPL2a and 2b which catalyze the intramembrane proteolysis of tumor necrosis factor alpha, ii) various proteins containing a C3H2C3 RING finger including, Arabidopsis ReMembR-H2 protein and various E3 ubiquitin ligases such as human GRAIL (gene related to anergy in lymphocytes), iii) EDEM3 (ER-degradation-enhancing mannosidase-like 3 protein), iv) various plant vacuolar sorting receptors such as Pisum sativum BP-80, v) glutamate carboxypeptidase II (GCPII), vi) yeast aminopeptidase Y, vii) Vibrio metschnikovii VapT, a sodium dodecyl sulfate (SDS) resistant extracellular alkaline serine protease, viii) lactocepin (a cell envelope-associated protease from Lactobacillus paracasei subsp. paracasei NCDO 151), ix) various subtilisin-like proteases such as melon Cucumisin, and x) human TfR (transferrin receptor) 1 and 2.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCA23124.1|GH47	1.19e-253	10	646	8	643
AIG56058.1|GH47	5.10e-232	13	653	11	641
CDM84177.1|GH47	6.21e-132	29	527	39	510
CDM84423.1|GH47	6.21e-132	29	527	39	510
BBM97073.1|GH47	1.91e-130	29	514	36	481

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5KIJ_A	2.09e-49	40	463	11	435	Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase and Man9GlcNAc2-PA complex [Homo sapiens]
5KK7_A	2.23e-49	40	463	11	435	Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase T688A mutant and Thio-disaccharide substrate analog complex [Homo sapiens],5KK7_B Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase T688A mutant and Thio-disaccharide substrate analog complex [Homo sapiens]
1FMI_A	2.38e-49	40	463	16	440	Crystal Structure Of Human Class I Alpha1,2-Mannosidase [Homo sapiens]
1FO2_A	2.48e-49	40	463	16	440	Crystal Structure Of Human Class I Alpha1,2-Mannosidase In Complex With 1-Deoxymannojirimycin [Homo sapiens],1FO3_A Crystal Structure Of Human Class I Alpha1,2-Mannosidase In Complex With Kifunensine [Homo sapiens]
1X9D_A	1.11e-48	40	463	94	518	Crystal Structure Of Human Class I alpha-1,2-Mannosidase In Complex With Thio-Disaccharide Substrate Analogue [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9FG93|MNS4_ARATH	4.09e-122	30	507	36	473	Alpha-mannosidase I MNS4 OS=Arabidopsis thaliana OX=3702 GN=MNS4 PE=1 SV=1
sp|Q92611|EDEM1_HUMAN	5.75e-121	34	462	130	568	ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Homo sapiens OX=9606 GN=EDEM1 PE=1 SV=1
sp|Q925U4|EDEM1_MOUSE	7.03e-121	34	462	125	563	ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Mus musculus OX=10090 GN=Edem1 PE=1 SV=1
sp|Q8BJT9|EDEM2_MOUSE	7.45e-115	28	456	29	441	ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Mus musculus OX=10090 GN=Edem2 PE=1 SV=1
sp|Q9BV94|EDEM2_HUMAN	2.13e-114	28	456	29	441	ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Homo sapiens OX=9606 GN=EDEM2 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000235	0.999726	CS pos: 24-25. Pr: 0.9447

TMHMM  Annotations     

There is no transmembrane helices in EPrPAT00000014678-p1.