CAZyme Information: EKG17518.1

Basic Information

• Species: Macrophomina phaseolina
• Lineage: Ascomycota; Dothideomycetes; ; Botryosphaeriaceae; Macrophomina; Macrophomina phaseolina
• CAZyme ID: EKG17518.1
• CAZy Family: GH43
• CAZyme Description: Six-hairpin glycosidase-like protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
607		69569.23	6.0626

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_MphaseolinaMS6	14712	1126212	906	13806

• Gene Location: location=AHHD01000234:45627-47555(-)

Full Sequence      

MVERRIIPDNPQATFPATRFDQESFCGRKRDVVFRNTLLYQLKVLKDTGRYDAFKLRWHH
SYSDEPDVPPIPNHLFWDSDVAKWIEGACYFLHERRNVEIETAVDELVDMIRSAQQPDGY
LNIHFIVVEPDKRFTNLRDLHELYNAGHLIEAALAHEQLFHNGKLLDPLIKYVDLLHATL
GPQPHQLHGYPGHPEIELALLRLYKTTHNPKHLELAAYFLDERGNPEGVDRRHYFDVESE
KRGERFNERPHYYPQRRSYWYQQAHARIVEQQTIEGHSVRAMYLLTAAADLVKMEDGREE
LEQAVLRLWKNMVERKMYLTGGIGAIKQWEGFGIDYFLPQGTDEGGCYAETCASIGAMML
AERLLQISLDGCFADIMELSFYNAVLTGMSCDGRAFTYVNQLCSSGNDLSKRSEWFTCAC
CPPNVARLLGYLGGYFWTARVEQAENRVSVDVYMYGSATLKIPVGTSAVTVKQASDWPWD
GKISFQVDAPEDIDVNLSLRIPSWASDWQVRLENIELPFLALTNPKLPRQFSGLPLSKGF
VCIPGSLLKSNLNFDLKIALKPRIICPHPYTNQDIIALARGPIIYCVEDVDNPWVDDHFK
VRGTFCS

Enzyme Prediction     

EC	3.2.1.185:2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	23	586	7e-165	0.9904580152671756

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
226063	COG3533	1.61e-126	14	601	13	521	Uncharacterized conserved protein, DUF1680 family [Function unknown].
400342	Glyco_hydro_127	4.06e-102	57	586	27	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ATZ54433.1|GH127	4.24e-295	11	607	4	589
CCD50963.1|GH127	4.73e-295	11	607	4	589
QYT01986.1|GH127	5.41e-283	11	600	4	577
QIW96336.1|GH127	5.53e-250	11	600	4	589
QKD55520.1|GH127	1.34e-239	10	603	3	588

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	6.90e-116	22	592	19	566	Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	2.75e-111	22	592	19	566	Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	4.64e-88	75	593	72	573	The crystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	5.98e-88	75	593	72	573	Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	1.64e-87	75	593	72	573	Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|E8MGH8|HYBA1_BIFL2	2.39e-87	75	593	72	573	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000053	0.000000

TMHMM  Annotations     

There is no transmembrane helices in EKG17518.1.