CAZyme Information: EKG17015.1

Basic Information

• Species: Macrophomina phaseolina
• Lineage: Ascomycota; Dothideomycetes; ; Botryosphaeriaceae; Macrophomina; Macrophomina phaseolina
• CAZyme ID: EKG17015.1
• CAZy Family: GH35
• CAZyme Description: Pectin lyase fold/virulence factor

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
736	AHHD01000257|CGC1	79010.30	4.1849

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_MphaseolinaMS6	14712	1126212	906	13806

• Gene Location: location=AHHD01000257:9890-12863(+)

Full Sequence      

MRSTVVLWVVLPLVAAVVAAPDRLARRQWEESDVNSWEPDWDSNLDLSPTTATGSSPAPV
FTQDAWPPFPYESSTLASEPPICTPLIPTYSGSWWYTTLDHSTAHSAFLPPIANQTYPIH
RNVLDYSADPTGQFSSTAAIQAAIDAGGPFLPASFRNTSTRGARYGSTLFPAVVHVPPGT
YVLDGPLQLTLSGYNSNIIINDLTFYGGAIGIELSGQQWAVKSVSFNWCGIGAVVDCFDC
VFVDITCIGVGICLDGSQTSGSLTVLDGWVAMTGTFVSSYASVDGYNQLVLENIESDGGD
TVTLSGNVVVAGSIPYTWIRGQMYSKGNPYPSRQQGATVYTPRSSALLNGNNYFVMKPPT
FSEYDSSQILNIKSLPDYPVFGDGSTDDTDSINAILNQYAGCKVIYFPAGTYIVQSTIFV
PRDTRMIGDAYASLISARGPIFSNPQNPIPMIQIGNPGDVGVAQIIDMMFTVAEVLPGCK
LVEVNMAGAAPGAVALINSHIRIGGAAGSTVQTSCGGSPDACRAAWGLVHLTAWSSAYVE
NMWGWTADHDLDDVFEQNIATGRGMLVEATRGTWLVGTAMEHNTLYQYNFHGAQNVFAAL
QQSETPYWQGIDSQTVAPWPWTYDLLPSDPDFAHCGDGDGGYCGAALFEKISASSGLFLY
GGCLWTFFTGNESKRCRYDCQQEAIDVDDTSNGIYLYGTNVHKVTNMVRSGEWSIAGAST
NEGGWGGVVAAYLYNS

Enzyme Prediction     

No EC number prediction in EKG17015.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH55	192	732	3.8e-149	0.7324324324324324

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
403800	Pectate_lyase_3	3.08e-11	121	236	2	193	Pectate lyase superfamily protein. This family of proteins possesses a beta helical structure like Pectate lyase. This family is most closely related to glycosyl hydrolase family 28.
403800	Pectate_lyase_3	5.02e-07	377	432	6	64	Pectate lyase superfamily protein. This family of proteins possesses a beta helical structure like Pectate lyase. This family is most closely related to glycosyl hydrolase family 28.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGA21782.1|GH55	1.59e-210	83	736	87	822
BCS06656.1|GH55	6.01e-204	70	736	74	819
BCR94046.1|GH55	6.01e-204	70	736	74	819
EAA60087.1|GH55	8.13e-199	39	735	138	933
CAK47731.1|GH55	3.55e-197	70	736	201	945

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3EQN_A	3.86e-71	117	735	46	758	Chain A, Glucan 1,3-beta-glucosidase [Phanerodontia chrysosporium],3EQN_B Chain B, Glucan 1,3-beta-glucosidase [Phanerodontia chrysosporium],3EQO_A Chain A, Glucan 1,3-beta-glucosidase [Phanerodontia chrysosporium],3EQO_B Chain B, Glucan 1,3-beta-glucosidase [Phanerodontia chrysosporium]
5M5Z_A	6.53e-64	94	730	6	751	Chain A, Beta-1,3-glucanase [Thermochaetoides thermophila],5M60_A Chain A, Beta-1,3-glucanase [Thermochaetoides thermophila]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4B0V1|E13B_ARTBC	1.09e-68	200	732	234	873	Probable glucan endo-1,3-beta-glucosidase ARB_02077 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02077 PE=1 SV=1
sp|P53626|E13B_TRIHA	6.82e-63	286	732	305	760	Glucan endo-1,3-beta-glucosidase BGN13.1 OS=Trichoderma harzianum OX=5544 GN=bgn13.1 PE=1 SV=1
sp|P49426|EXG1_COCCA	1.52e-60	202	732	241	777	Glucan 1,3-beta-glucosidase OS=Cochliobolus carbonum OX=5017 GN=EXG1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000253	0.999704	CS pos: 19-20. Pr: 0.9737

TMHMM  Annotations     

There is no transmembrane helices in EKG17015.1.