CAZyme Information: EKG16964.1

Basic Information

• Species: Macrophomina phaseolina
• Lineage: Ascomycota; Dothideomycetes; ; Botryosphaeriaceae; Macrophomina; Macrophomina phaseolina
• CAZyme ID: EKG16964.1
• CAZy Family: GH32
• CAZyme Description: Galactosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
344	AHHD01000258|CGC1	37505.73	4.2020

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_MphaseolinaMS6	14712	1126212	906	13806

• Gene Location: location=AHHD01000258:50205-51316(-)

Full Sequence      

MAWTELLETDFLHVDNLTDSGWVPQVYNVSATAARGAYGKMATLDNVIANPLKNSWDWAG
EAESGGDAGLQLVVRSQVVEELVPIGEVVAARTDMLFGSFRVAMKLTGVPGTCGAFFWFR
NDTQEIDIEFLSKQLNESSNMVNLVLQSPQSAAAGYDASHTPNYEVYPLPFRPDKAFHEY
RFDWLPDRVSFYADGTWLKDMTIDLPNSPGHLVLNHWSNGDPLWSAGPPREDATLTVSYV
KAYFNSSDAQRQKDYYLRCQDEGAPNAVCVIPEQTTPPDNQGPDANVTAKTFFFSEEPSD
TANQTVFGDWQNKNGAVGVGAPAAWVVAAGVAFASAMALVGGVA

Enzyme Prediction     

No EC number prediction in EKG16964.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	61	243	2e-83	0.9635416666666666

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185683	Glyco_hydrolase_16	1.41e-39	66	243	36	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
185684	GH16_lichenase	1.80e-15	97	239	68	205	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
395585	Glyco_hydro_16	3.84e-08	92	241	28	168	Glycosyl hydrolases family 16.
225182	BglS	5.33e-08	96	311	117	325	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185692	GH16_fungal_CRH1_transglycosylase	2.48e-04	95	222	45	169	glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBX96484.1|GH16_23	1.57e-118	2	322	41	356
QRD03541.1|GH16_23	8.90e-116	2	315	41	355
CAE7022543.1|GH16_23	2.54e-108	2	305	42	343
CCD51958.2|GH16_23	3.53e-106	3	307	41	350
ATZ52796.1|GH16_23	3.53e-106	3	307	41	350

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1CPN_A	2.80e-11	97	218	14	127	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1MAC_A	2.97e-11	97	218	70	183	Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans],1MAC_B Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans]
1BYH_A	3.06e-11	97	218	72	185	MOLECULAR AND ACTIVE-SITE STRUCTURE OF A BACILLUS (1-3,1-4)-BETA-GLUCANASE [synthetic construct],1GLH_A Cation Binding To A Bacillus (1,3-1,4)-Beta-Glucanase. Geometry, Affinity And Effect On Protein Stability [Paenibacillus macerans],2AYH_A Crystal And Molecular Structure At 1.6 Angstroms Resolution Of The Hybrid Bacillus Endo-1,3-1,4-Beta-D-Glucan 4- Glucanohydrolase H(A16-M) [hybrid]
1CPM_A	3.06e-11	97	218	14	127	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
3O5S_A	1.05e-10	97	218	96	209	Crystal Structure of the endo-beta-1,3-1,4 glucanase from Bacillus subtilis (strain 168) [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P23904|GUB_PAEMA	2.15e-10	97	218	95	208	Beta-glucanase OS=Paenibacillus macerans OX=44252 PE=1 SV=2
sp|P07980|GUB_BACAM	4.03e-10	97	218	97	210	Beta-glucanase OS=Bacillus amyloliquefaciens OX=1390 GN=bglA PE=3 SV=1
sp|P04957|GUB_BACSU	5.66e-10	97	218	100	213	Beta-glucanase OS=Bacillus subtilis (strain 168) OX=224308 GN=bglS PE=1 SV=2
sp|Q84C00|GUB_ACETH	1.17e-09	97	245	103	245	Beta-glucanase OS=Acetivibrio thermocellus OX=1515 GN=licB PE=1 SV=1
sp|P45797|GUB_PAEPO	1.34e-09	97	218	96	209	Beta-glucanase OS=Paenibacillus polymyxa OX=1406 GN=gluB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000033	0.000003

TMHMM  Annotations     

Start	End
321	343