CAZyme Information: EKG16418.1

Basic Information

• Species: Macrophomina phaseolina
• Lineage: Ascomycota; Dothideomycetes; ; Botryosphaeriaceae; Macrophomina; Macrophomina phaseolina
• CAZyme ID: EKG16418.1
• CAZy Family: GH3
• CAZyme Description: Glycoside hydrolase family 5

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
753	AHHD01000269|CGC2	84892.19	4.8400

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_MphaseolinaMS6	14712	1126212	906	13806

• Gene Location: location=AHHD01000269:95814-98184(+)

Full Sequence      

MAPLRLRIDGRTFRDQSNREVTLHGINVAGDAKFPANPDQPSYVADDFFDADNVSFVGRP
FTVDTARTHFTRLRKMGFNTIRYIFTWEAIEHAGPKKYDEDWIQHTISILRVAKEFGFYV
FMDPHQDVWSRFSGGSGAPLWTVYACGLNPRAFDITEAALVQNTYPDPEKFPKMIWATNY
TRMACQVIWTLFFAGKAFAPKAIIDGVNIQDYLQNHFLDALIHLARRIQEAGDLDNEVII
GWESINEPNRGLIGWGDLSIIPKEQKLQKGTSPTAWQAILTGSGRACEIDTWDFGGMGPY
KSGSVLVDPQGQSAWLPSDYDDTHYGFKRDPGWKLGECLWAQHGIWDPESDTLLKKEYFS
KDPENGEAVDYEYFTNKWFMQYYRRHRDAIRSIFPDTIMFCQPPVLEIPPTIKGTEDDDP
NMVFTPHFYDGVTLMTKKWNRLWNVDVFGVLRGKYLTPAFAIKIGETAIRNCFRDQLAAI
RQEGLDYMGIHPCVFSEIGIPYDMDDRYAYRTGDYTSQTLAMDANHFALEASHATGFTLW
AYVATNNHEWGDRWNGEDLSIFSLDDPTLPTLSSPTADPESPSYSRSQSSETSRVSPSNL
KQTLGEDEMTSSAMSSASKSDKPGYRAAEAYVRPTPTLTHGTILNYGFDLRNCVFTLSLE
APSSSGEGGPTEVFLPEFHFPQGSVSVEVSGGKWTISMEDTAAGPIQMLRWWHAEGEQKM
TVTGVKSRQALAAEDDGYIEQCQQTAGQKCVVM

Enzyme Prediction     

EC	3.2.1.21:3	3.2.1.45:1	3.2.1.104:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH5	15	548	1.2e-220	0.9981447124304267

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408348	Glyco_hydro_5_C	1.89e-29	633	722	1	86	Glycoside hydrolase family 5 C-terminal domain. This is the C-terminal domain of endo-glycoceramidase II (EGC), a membrane-associated family 5 glycosidase pfam00150. The C-terminal domain assumes a beta-sandwich fold, which resembles that of many carbohydrate-binding modules.
395098	Cellulase	0.004	70	127	30	88	Cellulase (glycosyl hydrolase family 5).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRD01159.1|GH5_12	0.0	1	753	1	755
QDS71373.1|GH5_12	0.0	1	741	1	753
CAE7210891.1|GH5_12	0.0	1	753	1	756
CBX97977.1|GH5_12	0.0	1	753	1	756
APA14319.1|GH5_12	0.0	1	745	1	758

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LPO_A	6.88e-194	13	741	28	784	Chain A, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPO_B Chain B, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPO_C Chain C, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPO_D Chain D, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_A Chain A, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_B Chain B, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_C Chain C, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPP_D Chain D, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_A Chain A, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_B Chain B, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_C Chain C, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99],7LPQ_D Chain D, Cytoplasmic protein [Cryptococcus neoformans var. grubii H99]
2OSW_A	3.12e-07	13	329	37	289	Endo-glycoceramidase II from Rhodococcus sp. [Rhodococcus sp.],2OSW_B Endo-glycoceramidase II from Rhodococcus sp. [Rhodococcus sp.],2OYK_A Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like isofagomine complex [Rhodococcus sp.],2OYK_B Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like isofagomine complex [Rhodococcus sp.],2OYL_A Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like imidazole complex [Rhodococcus sp.],2OYL_B Endo-glycoceramidase II from Rhodococcus sp.: cellobiose-like imidazole complex [Rhodococcus sp.],2OYM_A Endo-glycoceramidase II from Rhodococcus sp.: five-membered iminocyclitol complex [Rhodococcus sp.],2OYM_B Endo-glycoceramidase II from Rhodococcus sp.: five-membered iminocyclitol complex [Rhodococcus sp.]
2OSX_A	3.12e-07	13	329	37	289	Chain A, Endoglycoceramidase II [Rhodococcus sp.]
2OSY_A	9.46e-07	13	148	37	161	Chain A, Endoglycoceramidase II [Rhodococcus sp.],2OSY_B Chain B, Endoglycoceramidase II [Rhodococcus sp.]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|H1AE13|EGCR1_ASPFM	0.0	1	753	1	759	Glucosylceramidase OS=Neosartorya fumigata OX=746128 GN=egc1 PE=1 SV=1
sp|P40566|EGH1_YEAST	1.14e-176	2	718	3	734	Ergosteryl-beta-glucosidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=EGH1 PE=1 SV=1
sp|I1BTD7|EGCR1_RHIO9	1.25e-115	10	693	19	641	Glucosylceramidase OS=Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) OX=246409 GN=ERC1 PE=1 SV=1
sp|H1AE12|EGCR1_CRYNH	3.64e-79	6	699	16	659	Glucosylceramidase OS=Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) OX=235443 GN=EGC1 PE=1 SV=1
sp|Q6L6S1|EGCSE_HYDVU	1.85e-07	15	262	34	245	Endoglycoceramidase OS=Hydra vulgaris OX=6087 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000056	0.000000

TMHMM  Annotations     

There is no transmembrane helices in EKG16418.1.