CAZyme Information: EKG16231.1

Basic Information

• Species: Macrophomina phaseolina
• Lineage: Ascomycota; Dothideomycetes; ; Botryosphaeriaceae; Macrophomina; Macrophomina phaseolina
• CAZyme ID: EKG16231.1
• CAZy Family: GH3
• CAZyme Description: Bacterial alpha-L-rhamnosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
904		101718.76	6.1542

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_MphaseolinaMS6	14712	1126212	906	13806

• Gene Location: location=AHHD01000283:25254-27968(-)

Full Sequence      

MSVQLIDVRFEHHPDGFGIGTATPRLSWRFSHGKTPAENWQQSGYTVEISRQEGTQTHSF
HVESSESVLNPWPTKPLTSRERVSVRVRAFGASERDGHQPDPAPTEWSHWHVVEAGLLQR
SDWTGNFIVSPLEEPKDKPHRPVLFRKSFDSPATKEARAWLYITSLGVYEAHINGRRVGD
LQMTPGWTSYRHRLQYQVFDVTELIQEGKNTIGVEVSEGWYAGRLTWGKGIRNFYGDKLA
TLVQLEIQTKDGVNQRIVSEESWKTHQSDLIMSELYDGEVYDARQHLQGWTSPTFIEGSN
WKPSCILEFPNILLFAPDVAPVRVTQSVQSREIFRSRSGKTLVDFGQNLVGKVQIKLLHK
PEGHTVKIRHAEVLEHGELGTRPLRNARAEDTVIFSNQPLKDWAPRFTFHGFRYVQLEGW
SLDDDVPPSTENIAALVMHSDLVRTGQFSCSNDLVNRLHENVVWSMRGNFLSIPTDCPQR
DERLGWTGDIQVFSPTASYLYQSAGFLSNWMQDVAAEQGDMDGIVPLVVPDVLGTTSWPS
VPQAVWDDVAILVPWVLYQWSGDVEIVRRQYSSMRVYLDTSIRRGDDGLWDPDLWQLGDW
LDPNAPPREPGLARTDGTLVADAYLVYVTGIMAKISDAVERPQETVLYQKQHDDLKAVFA
DKYIAPSGLLVGDSQTALALALAFSLHGESNPKQVRTAADRLARLVRYSKFRVSTGFAGT
PLVLHALSSTDHIQLAYRMLLEEQCPSWLYPVTMGATTIWERWDSMLPDGSINPGEMTSF
NHYALGSVANWLHTVVGGIRPLEPGWKKFLVSPVPGGTLTNAEVKFESPYGLVEAKWKLD
DGHTRFVLRLNVPPNTTALLVLPGQKAEEGVWIGSGSHVREASFKAPGNWPPKAMYTQFQ
EGLE

Enzyme Prediction     

EC	3.2.1.40:1	3.2.1.40:8	3.1.1.73:2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	333	862	1.9e-168	0.998015873015873
CBM67	127	304	1.1e-37	0.9204545454545454

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
407469	Bac_rhamnosid6H	9.01e-146	444	796	2	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
400715	Bac_rhamnosid_N	4.79e-68	157	326	4	172	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
336149	Bac_rhamnosid	1.70e-31	335	439	2	102	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
379972	Bac_rhamnosid_C	5.52e-23	798	871	1	72	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QMW40472.1|GH78	0.0	11	893	13	908
UDD56417.1|GH78	0.0	11	893	13	908
QRD82895.1|GH78	0.0	11	893	13	908
BAE58123.1|GH78	0.0	11	893	13	908
QMW28401.1|GH78	0.0	11	893	13	908

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6GSZ_A	2.68e-278	2	878	1	861	Crystal structure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]
3W5M_A	4.96e-167	146	878	306	1026	Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6I60_A	4.55e-129	7	863	34	899	Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q82PP4|RHA78_STRAW	1.82e-166	146	878	306	1026	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
sp|T2KNB2|PLH20_FORAG	7.23e-161	64	868	98	884	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
sp|P9WF03|RHA78_ALTSL	2.26e-137	18	863	45	879	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
sp|T2KPL4|PLH28_FORAG	3.87e-86	2	862	29	906	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000055	0.000000

TMHMM  Annotations     

There is no transmembrane helices in EKG16231.1.