CAZyme Information: EKG13216.1

Basic Information

• Species: Macrophomina phaseolina
• Lineage: Ascomycota; Dothideomycetes; ; Botryosphaeriaceae; Macrophomina; Macrophomina phaseolina
• CAZyme ID: EKG13216.1
• CAZy Family: CE4
• CAZyme Description: Glycoside hydrolase family 3

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
619	AHHD01000414|CGC1	66547.76	4.4500

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_MphaseolinaMS6	14712	1126212	906	13806

• Gene Location: location=AHHD01000414:9578-11620(+)

Full Sequence      

MGAVIAQEALALGINQIFAPVVDLARELRFGRVEETYGEDPYLAGEMGYAYVRALESQNV
SAMVKHFAGFSNPEQGLNTGPVHGGERELRTTWLPPFHRAIIDGGATSIMSAYHSWDGVP
AVADYHTLTEILREEWGYEYYVSSDAGGTDRLCNAFKMCRSNPIDKEAVTMYALPAGNDV
EMGGGSYNFETIIDLIGSGKLDIDIVDTAVARTLRTKFFLGLFEDPYRAVPANETALHIH
TAKSVDLARKLDAESIVLLENHDNVLPLDKSANIAVIGPMADFMNYGDYVVQDSQYRGVT
PYAGIAAASTGTVTYTLGTERWSTDTSGFPAAIAAAQAADVAVVVVGTWSRDQTQLWQGL
NATTGEHVDVHDLALVGAQAALVKAIIATGKPTVVVFQSGKPVTEPWISRNASALVQQFY
PGEQGGNALADVLFGDHNPSGKLAVGFPYDVGTTPVFYDYLNSGRTVDAGQVFDNGTLLF
GHQYVLSSPLPLYEFGYGKSYAEFAYSNVTLSKATASATDVVTATVSVTNNSTRDGQEVV
QLYVSDLIASVAVPNKELKGFKKVFIPAGETVDVAIDIDVSKLGVWDIRMKYVVEPGEFA
IWVGSSSADLRGNATLTVE

Enzyme Prediction     

EC	3.2.1.120:1	3.2.1.21:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	1	182	5.9e-51	0.7731481481481481

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185053	PRK15098	7.49e-135	1	611	138	758	beta-glucosidase BglX.
224389	BglX	5.54e-62	1	310	101	396	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
178629	PLN03080	1.58e-53	1	604	132	769	Probable beta-xylosidase; Provisional
396478	Glyco_hydro_3_C	1.07e-45	256	501	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
395747	Glyco_hydro_3	2.19e-44	1	215	106	315	Glycosyl hydrolase family 3 N terminal domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
APA15785.1|GH3	0.0	1	618	154	776
CCD56845.1|GH3	0.0	1	618	154	776
ATZ51038.1|GH3	0.0	1	618	154	776
QSZ32114.1|GH3	0.0	1	619	154	777
QDS77227.1|GH3	1.02e-312	1	619	158	780

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5YOT_A	1.83e-310	1	619	134	757	Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YOT_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_A Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40]
7EAP_A	1.49e-309	1	619	134	757	Chain A, Fn3_like domain-containing protein [Aspergillus oryzae RIB40]
6R5I_A	6.90e-100	6	614	111	729	Chain A, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5I_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_A Chain A, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5N_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1]
5TF0_A	1.05e-99	4	611	120	744	Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393],5TF0_B Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393]
6R5O_A	3.73e-99	6	614	111	729	Chain A, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5O_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5R_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5T_A Chain A, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5T_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5U_A Chain A, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5U_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5V_A Chain A, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1],6R5V_B Chain B, Periplasmic beta-glucosidase [Pseudomonas aeruginosa PAO1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q56078|BGLX_SALTY	8.25e-103	1	611	138	758	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
sp|P33363|BGLX_ECOLI	1.23e-101	1	611	138	758	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
sp|T2KMH0|PLH24_FORAG	7.73e-99	1	619	108	723	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
sp|T2KMH9|PLH34_FORAG	1.67e-72	4	611	162	748	Putative beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22230 PE=2 SV=1
sp|P14002|BGLB_ACET2	4.33e-71	1	619	88	675	Thermostable beta-glucosidase B OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000046	0.000012

TMHMM  Annotations     

There is no transmembrane helices in EKG13216.1.