CAZyme Information: EKG13022.1

Basic Information

• Species: Macrophomina phaseolina
• Lineage: Ascomycota; Dothideomycetes; ; Botryosphaeriaceae; Macrophomina; Macrophomina phaseolina
• CAZyme ID: EKG13022.1
• CAZy Family: CE16
• CAZyme Description: Glycosyltransferase ALG3

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
419		47387.03	9.8712

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_MphaseolinaMS6	14712	1126212	906	13806

• Gene Location: location=AHHD01000419:92336-94004(+)

Full Sequence      

MDLVKQGVSVATNPKYARWLCPLLLVADAALCGLIIWKVPYTEIDWKAYMEQVAQYLAGE
RDYTRIKGGTGPLVYPGAHVYIYRGLYSLTDEGRDIRLAQIIFAGLYVAVLTIVMACYRL
AKVPPYVFPMLVLSKRLHSIFVLRCFNDCFAVGAFFLAIYAYQKRMWTLGSICYSLAVGI
KMSALLALPAVGLILLQAIGKERAITQAGIIGYLQLLLGFPFLQTNARAYFSRAFELTRV
FLYKWTVNWRFVPEPVFLSKPFSLTLLFAHLLLLVLFASTRWLKPAQRPLLECVKMAFVE
PADQVHVARRINPRFVLTAMLSSVVVGMLCARSLHYQFYAWLAWATPFLAWRAGVHPVAQ
YALWAAQEWAWNVFPSTRASSGVVVAALALQVGGVWWGTRRDFEGRAEQQQQQQHAHVE

Enzyme Prediction     

EC	2.4.1.258:10

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT58	22	381	1.4e-148	0.9945054945054945

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
398745	ALG3	0.0	22	381	1	358	ALG3 protein. The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDS73095.1|GT58	1.01e-179	1	407	13	422
CBX89957.1|GT58	1.58e-175	1	405	89	494
QRC96987.1|GT58	8.79e-173	1	405	1	406
CAE7199648.1|GT58	1.84e-171	2	419	7	425
QIW98545.1|GT58	6.36e-167	1	399	1	401

PDB Hits     

EKG13022.1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9C1K8|ALG3_NEUCR	1.73e-161	3	404	14	427	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=alg-3 PE=3 SV=1
sp|Q2U6A4|ALG3_ASPOR	7.50e-146	1	400	1	396	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alg3 PE=3 SV=1
sp|A1CBE6|ALG3_ASPCL	3.54e-143	1	397	63	455	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=alg3 PE=3 SV=1
sp|Q4WVG2|ALG3_ASPFU	1.13e-141	1	395	9	399	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=alg3 PE=3 SV=1
sp|A1DDZ3|ALG3_NEOFI	8.01e-140	1	395	1	391	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=alg3 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000022	0.000021

TMHMM  Annotations     

Start	End
19	41
98	120
140	162
177	196
209	231
256	278
314	336
379	398