CAZyme Information: EKG12952.1

Basic Information

• Species: Macrophomina phaseolina
• Lineage: Ascomycota; Dothideomycetes; ; Botryosphaeriaceae; Macrophomina; Macrophomina phaseolina
• CAZyme ID: EKG12952.1
• CAZy Family: CE12
• CAZyme Description: Chromo shadow

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
717	AHHD01000422|CGC1	79088.46	5.3724

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_MphaseolinaMS6	14712	1126212	906	13806

• Gene Location: location=AHHD01000422:122359-125469(-)

Full Sequence      

MFSRLSYTAPALAALIPLATAQTYSSCNPTDSSQFPCPADEGLCQSAVFETDFTTGDNSS
WHSLAGTVSYTDAGAEFTIAQRGDAPTIETDFYIFYGKVEVTMKSAPGTGIVSSIVLESN
DLDEIDWEFLGGEDTLVQSNYFGKGNTTTYDREAEHAVATPEETSHTYTIDWTASQIVWS
IDGTAVRTLAYADAVSGTNFPQTPSTLRLGIWAGGDEDNSYWTRVWAGGNTTYTAAAGMP
WTMTVEKVNSNGEEVREIRLPERSRQFSSALSDISRLSMPRNTHMPKSLRSDYLSDRRPP
PSYAVRQSIKLAPETSWLSPSSPINKLSDILSYCDSTQISKMPHSVSSPSVQPLPKAPGS
DVDFGAVVTGLDVENLSDADFAFLHDALYQHSVLVIKSTPTLSPRAQYDLTRRFDPATDT
YGHGTTLDAKRSVLHPDLKTIPSQPQVQVIGNGHIASYAGLTDITLRHPHHRKFHAPPHV
PAELDLTHTRFFRWHIDAALYALNPPRVTSLLAVRVPGGRRQVCRYDDGSGDELPVPLGT
TAFASGYRMYELLSPADRELARTSRVEYAPHPYIWMSGAGSRPNGLGMVSEGKELREEEL
PPVEADKIMVLPMCWKNPVTGRLALQVHPSAVRRIHLRDGRVVEDLEEVRKILYRMQRPG
IAPQYVYPHDWEEGDLVLFNNHGVIHSVVGAFAEDEVRLFRQCNLAASTRPAGPDDE

Enzyme Prediction     

No EC number prediction in EKG12952.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	73	229	1.5e-68	0.9873417721518988

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185692	GH16_fungal_CRH1_transglycosylase	1.17e-102	51	249	1	198	glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
395585	Glyco_hydro_16	1.92e-48	65	228	3	168	Glycosyl hydrolases family 16.
367137	TauD	4.23e-34	350	703	1	264	Taurine catabolism dioxygenase TauD, TfdA family. This family consists of taurine catabolism dioxygenases of the TauD, TfdA family. TauD from E. coli is a alpha-ketoglutarate-dependent taurine dioxygenase. This enzyme catalyzes the oxygenolytic release of sulfite from taurine. TfdA from Burkholderia sp. is a 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase. TfdA from Alcaligenes eutrophus JMP134 is a 2,4-dichlorophenoxyacetate monooxygenase. Also included are gamma-Butyrobetaine hydroxylase enzymes EC:1.14.11.1.
225086	TauD	3.79e-27	363	714	13	285	Taurine dioxygenase, alpha-ketoglutarate-dependent [Secondary metabolites biosynthesis, transport and catabolism].
185683	Glyco_hydrolase_16	2.03e-24	47	248	7	207	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAK37281.1|GH16_18	1.32e-87	6	249	4	240
BCS06099.1|GH16_18	4.26e-87	6	249	4	240
BCR93457.1|GH16_18	4.26e-87	6	249	4	240
CAA11266.1|GH16_18|2.4.1.-	1.11e-86	7	254	15	259
AAN87849.1|GH16_18|2.4.1.-	2.05e-85	7	254	5	249

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6IBU_A	7.34e-81	24	254	1	228	Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBU_B Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBW_A Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293],6IBW_B Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293]
1CPN_A	1.18e-12	96	252	14	157	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1MAC_A	1.72e-12	96	233	70	201	Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans],1MAC_B Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans]
1CPM_A	1.79e-12	96	233	14	145	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1BYH_A	1.79e-12	96	233	72	203	MOLECULAR AND ACTIVE-SITE STRUCTURE OF A BACILLUS (1-3,1-4)-BETA-GLUCANASE [synthetic construct],1GLH_A Cation Binding To A Bacillus (1,3-1,4)-Beta-Glucanase. Geometry, Affinity And Effect On Protein Stability [Paenibacillus macerans],2AYH_A Crystal And Molecular Structure At 1.6 Angstroms Resolution Of The Hybrid Bacillus Endo-1,3-1,4-Beta-D-Glucan 4- Glucanohydrolase H(A16-M) [hybrid]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q7SEI7|XANA_NEUCR	1.43e-179	342	717	1	382	Alpha-ketoglutarate-dependent xanthine dioxygenase xan-1 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=xan-1 PE=1 SV=1
sp|C8VSZ2|XANA_EMENI	1.86e-174	350	707	5	361	Alpha-ketoglutarate-dependent xanthine dioxygenase xanA OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=xanA PE=1 SV=2
sp|Q4QZZ9|XANA_EMEND	1.86e-174	350	707	5	361	Alpha-ketoglutarate-dependent xanthine dioxygenase xanA OS=Emericella nidulans OX=162425 GN=xanA PE=1 SV=1
sp|O74885|XANA_SCHPO	3.97e-129	353	717	40	408	Alpha-ketoglutarate-dependent xanthine dioxygenase xan1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=xan1 PE=1 SV=2
sp|Q8J0P4|CRF1_ASPFU	3.65e-86	7	254	5	249	Probable glycosidase crf1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=crf1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000255	0.999724	CS pos: 21-22. Pr: 0.9777

TMHMM  Annotations     

There is no transmembrane helices in EKG12952.1.