CAZyme Information: EKG12630.1

Basic Information

• Species: Macrophomina phaseolina
• Lineage: Ascomycota; Dothideomycetes; ; Botryosphaeriaceae; Macrophomina; Macrophomina phaseolina
• CAZyme ID: EKG12630.1
• CAZy Family: CBM63
• CAZyme Description: Pectin lyase fold/virulence factor

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
791		84834.53	4.5855

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_MphaseolinaMS6	14712	1126212	906	13806

• Gene Location: location=AHHD01000446:181273-183698(-)

Full Sequence      

MFAFSTAALALALRFIAFSAGPVNAVPTPQLVDVQVGASPAGVTVQADAAAAASSYWVAN
IERNGAPAFGGADFKVFRNVKDYGATGDGSTDDTAAINSAISAGNRCGKGCDSSTVTPAI
VYFPPGTYVVSKPIVQYYYTQIIGDKLDLPVIKAAAGFTGMAVIDADPYENDGSNWYTNQ
NNFFRQIRNLVIDLTAMPPSSGAGIHWQVAQATSLQNIRFEMVKGGADNKQQGIFMDNGS
GGFMTDLTFNGGNYGAFLGSQQFTTRNMTFNDCNTAIFMNWNWLWTFHGLSINNCKVGID
MANAPANQTVGSVIVLDSTFTNTPLGVNTSFSDNSVPATGGTLVLENVDFSGSQTAVLSA
GTEKLAGGSTVASWVQGKEYIGSAGSRIQKEVDFTKPTPLLGTDKKIFTRSKPQYETYAA
DAFVSIKKAGAKGDGKNDDTAIIQKAINDLKDEQILYFDQGAYLITDTITIPGNKNIKIT
GEIWPELLASGTKFSDQNNPVPVFKVGNANDTGRVEMSDLVIGTQGPTPGAILMQWNLNA
EPGAGALWDVHFRVAGYAGTKLQSDTCAKNPNSTHDANPECEAAFLLFHATETASVYVEN
CWFWVADHELDLSDHNQIDIYNGRGVLIESKNPVWLWGTASEHNVLYNYQLKGAKNVFMG
HIQTETPYYQSNPDATKPFTVQTSWSDPDFSSASAEGGKKAWGLRIIDSEDVVIYGGGLY
SFFENYGQECVSANNCQNNMVSYEGTNSNVQLFGLSTKAAVSMVTVQGNGVVKDADNRSN
FCATIGHFAES

Enzyme Prediction     

EC	3.2.1.58:16

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH55	48	788	3.5e-304	0.9905405405405405

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
403800	Pectate_lyase_3	3.38e-99	77	300	1	213	Pectate lyase superfamily protein. This family of proteins possesses a beta helical structure like Pectate lyase. This family is most closely related to glycosyl hydrolase family 28.
403800	Pectate_lyase_3	1.16e-07	423	561	1	143	Pectate lyase superfamily protein. This family of proteins possesses a beta helical structure like Pectate lyase. This family is most closely related to glycosyl hydrolase family 28.
227721	Pgu1	1.09e-04	79	129	84	125	Polygalacturonase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QMW47168.1|GH55	0.0	9	791	12	950
QRD91479.1|GH55	0.0	9	791	12	950
QMW35104.1|GH55	0.0	9	791	12	950
BAE64877.1|GH55	0.0	9	791	12	950
UDD64170.1|GH55	0.0	9	791	12	950

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5M5Z_A	0.0	52	788	2	753	Chain A, Beta-1,3-glucanase [Thermochaetoides thermophila],5M60_A Chain A, Beta-1,3-glucanase [Thermochaetoides thermophila]
3EQN_A	5.61e-213	50	785	20	752	Chain A, Glucan 1,3-beta-glucosidase [Phanerodontia chrysosporium],3EQN_B Chain B, Glucan 1,3-beta-glucosidase [Phanerodontia chrysosporium],3EQO_A Chain A, Glucan 1,3-beta-glucosidase [Phanerodontia chrysosporium],3EQO_B Chain B, Glucan 1,3-beta-glucosidase [Phanerodontia chrysosporium]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4B0V1|E13B_ARTBC	0.0	22	788	24	873	Probable glucan endo-1,3-beta-glucosidase ARB_02077 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02077 PE=1 SV=1
sp|P49426|EXG1_COCCA	7.03e-207	49	789	41	785	Glucan 1,3-beta-glucosidase OS=Cochliobolus carbonum OX=5017 GN=EXG1 PE=1 SV=1
sp|P53626|E13B_TRIHA	3.87e-54	16	777	8	744	Glucan endo-1,3-beta-glucosidase BGN13.1 OS=Trichoderma harzianum OX=5544 GN=bgn13.1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000214	0.999743	CS pos: 25-26. Pr: 0.9769

TMHMM  Annotations     

There is no transmembrane helices in EKG12630.1.