CAZyme Information: EJT82354.1

Basic Information

• Species: Gaeumannomyces tritici
• Lineage: Ascomycota; Sordariomycetes; ; Magnaporthaceae; Gaeumannomyces; Gaeumannomyces tritici
• CAZyme ID: EJT82354.1
• CAZy Family: GT4
• CAZyme Description: chitobiosyldiphosphodolichol beta-mannosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
481		52337.89	8.5488

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_GtriticiR3-111a-1	14749	644352	291	14458

• Gene Location: location=GL385395:7377506-7379895(-)

Full Sequence      

MLLPFILLCAIASCVALCAIASCVALFVLLPSRFRRSPQTVLESKPNVSVQVLVLGDIGR
SPRMQYHAMSIAKHGGRVDLIGYQETTPHPALLGHPKIKIHSLDLPPRVLRSKSIPFIIS
GPLKVIWQVFTLVHVLGYEAPPAQWLLIQNPPSIPTLAIAAIIARCRNTRLLIDWHNYGW
TILSGTRGAGHPFVFVSKLYECFFGRAGSANLTVTHAMARQLREKPYRIRSPIVTMHDRP
PDIFQPINNPTTRLDILARVLESRELAAAVVDGYVRLVVSSTSWTPDEDFNLLLSALVQY
ANDTQRSRPLSSLSLSSSSPPPPPTPVVAVITGKGPQKAMYEGKIAKMMADGLVPGVAIR
TAFLSYEDYAALLASADLGVCLHMSSSGVDLPMKVVDMFGAGLPVVAYSGYESFSELVKE
GHNGCGFETAGELAACLVRLLGVDGQAELAALRRGATQEGSRRWDEEWDTSVARILGFVS
E

Enzyme Prediction     

EC	2.4.1.142:10	2.4.1.-:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT33	49	473	1.9e-148	0.9882352941176471

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340843	GT33_ALG1-like	0.0	45	476	1	411	chitobiosyldiphosphodolichol beta-mannosyltransferase and similar proteins. This family is most closely related to the GT33 family of glycosyltransferases. The yeast gene ALG1 has been shown to function as a mannosyltransferase that catalyzes the formation of dolichol pyrophosphate (Dol-PP)-GlcNAc2Man from GDP-Man and Dol-PP-Glc-NAc2, and participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. In humans ALG1 has been associated with the congenital disorders of glycosylation (CDG) designated as subtype CDG-Ik.
215155	PLN02275	9.42e-133	50	441	7	371	transferase, transferring glycosyl groups
223515	RfaB	1.80e-12	55	481	14	376	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
340831	GT4_PimA-like	3.69e-11	59	478	15	364	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.
340844	GT4_UGDG-like	1.66e-06	326	436	231	333	UDP-Glc:1,2-diacylglycerol 3-a-glucosyltransferase and similar proteins. This family is most closely related to the GT1 family of glycosyltransferases. UDP-glucose-diacylglycerol glucosyltransferase (EC 2.4.1.337, UGDG; also known as 1,2-diacylglycerol 3-glucosyltransferase) catalyzes the transfer of glucose from UDP-glucose to 1,2-diacylglycerol forming 3-D-glucosyl-1,2-diacylglycerol.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBZ55236.1|GT33	2.33e-250	5	479	4	455
AEO55016.1|GT33	3.31e-181	48	480	52	487
UQC76213.1|GT33	8.58e-177	29	479	30	466
VBB84667.1|GT33	3.74e-174	28	481	18	450
UPK97115.1|GT33	1.46e-171	29	481	29	459

PDB Hits     

EJT82354.1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4AZD1|ALG1_ARTBC	2.70e-118	29	480	22	447	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01551 PE=3 SV=1
sp|Q8L7M0|TUN_ARATH	4.25e-88	52	476	9	455	UDP-glycosyltransferase TURAN OS=Arabidopsis thaliana OX=3702 GN=TUN PE=2 SV=1
sp|Q6C3K2|ALG1_YARLI	6.24e-87	52	476	44	438	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=ALG1 PE=3 SV=1
sp|Q921Q3|ALG1_MOUSE	3.89e-83	12	476	3	461	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Mus musculus OX=10090 GN=Alg1 PE=1 SV=3
sp|O13933|ALG1_SCHPO	1.19e-82	52	476	29	420	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=alg1 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000030	0.000019

TMHMM  Annotations     

Start	End
7	29