CAZyme Information: EJT79730.1

Basic Information

• Species: Gaeumannomyces tritici
• Lineage: Ascomycota; Sordariomycetes; ; Magnaporthaceae; Gaeumannomyces; Gaeumannomyces tritici
• CAZyme ID: EJT79730.1
• CAZy Family: GH81
• CAZyme Description: Glyco_hydro_2 domain-containing protein [Source:UniProtKB/TrEMBL;Acc:J3NU59]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
951		106219.48	6.8916

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_GtriticiR3-111a-1	14749	644352	291	14458

• Gene Location: location=GL385396:6651837-6654692(+)

Full Sequence      

MARAVTRLLSQGWEVKLGAPPGSEGAEDRWLPAGHLPTEIHLDLFRNGVIPDPHLDFNEL
AVRWVADRQWTYRCRFHAPPPSGTTDLVLEGLDTFAAVSLNGDEILVSDNMFVEHRIDVT
EKLRPGTSPSGEAANELVIAFDSARKRGLQLVKEHPEHRFIVHQTEISRGPVRKAQYQWG
WDWGPILQTCGPWKPIKLETFVTRIGDARISYRLAKDLDSARVTVKVQTRGAAPPARCCI
TLNDDLVYSGEPSIASRGEEFKVDFDIKKPQLWWPRGFGKQNMYKIKVSIMSHDGDVLDT
VGKNIGFRKAELVREKDAFGESFYLRVNGVDIFCGGSNWIPADSFLTRTTPDQYRDWVFS
HVAEGNQAMVRVWGGGIYEHDVFFDACDEAGILVWQDFAFACANYPTYPSFLSSVETEAR
QNVRRLRHHPSLVIWAGNNEDYQIVERYKLKYDFDDKDPQSWLKTDFPARYIYEHLLPKI
VEEEHAGAIYRPSSPWGNGTSTTLVVDATVGDVHEWDLWHGAMKPHQLLPGMGGRFISEF
GMEAYPHVPTLSRAVTDPAQRSRPGTLALDFRNKAIGHQRRLLAYLGENVDLHTPTGSTA
APSSLDGFTYLTQVVQADAMRSAYGGWRRRWGDETGARRCGGVLAWQLNDCWPTVSWSLV
DHDGVRKPAWYAVREALRPVVVCVRRPFFDCASRPADGGRTTWQRDTGHVDPRAAWGFGL
GAVGHHRAVSDLWVSNLGADEREGELQVSFVSIKTGKVLRRRLFARPLALRIQANGTTEV
SARPRREGGRRRSSAAAANKAGGGNPAATASADDEDSSPMDPTDADPFVMHAQLSTWPVG
GESKTAAAAESVHDVSWPDPLKYIDFSNRGLKVTAGESGGSQGDKRAWVEVKAERPVKCF
VFQERPGVRLSDNGFDVIPGHPVRVGVDGCHPRELKWMYLGQERQEADWAD

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	6	688	2.4e-85	0.6156914893617021

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	5.95e-61	34	680	32	632	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
395572	Glyco_hydro_2	7.96e-12	203	308	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
397119	Glyco_hydro_2_C	4.18e-04	380	442	61	137	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
407625	Ig_mannosidase	0.002	881	927	9	56	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBZ66458.1|GH2	0.0	6	951	5	906
AEO59187.1|GH2	0.0	1	942	1	897
AEO64472.1|GH2	7.33e-316	9	942	9	881
QRD07513.1|GH2	3.27e-314	9	941	14	879
CBX98985.1|CBM35|GH2|GH27	4.34e-309	9	940	622	1495

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	6.71e-125	27	682	40	658	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	3.72e-108	8	682	10	656	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	3.89e-108	8	682	12	658	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	3.98e-108	8	682	12	658	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	1.03e-107	8	682	10	656	Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5B7W2|MANBB_EMENI	4.20e-263	1	942	1	843	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2
sp|A1CGA8|MANBB_ASPCL	2.63e-259	9	942	9	845	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
sp|Q4WAH4|MANBB_ASPFU	1.09e-255	9	942	9	845	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
sp|B0YBU9|MANBB_ASPFC	4.36e-255	9	942	9	845	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
sp|A1D911|MANBB_NEOFI	8.73e-255	9	942	9	845	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000074	0.000000

TMHMM  Annotations     

There is no transmembrane helices in EJT79730.1.