CAZyme Information: EJT46175.1

Basic Information

• Species: Trichosporon asahii
• Lineage: Basidiomycota; Tremellomycetes; ; Trichosporonaceae; Trichosporon; Trichosporon asahii
• CAZyme ID: EJT46175.1
• CAZy Family: AA3
• CAZyme Description: UDP-glucose:glycoprotein glucosyltransferase [Source:UniProtKB/TrEMBL;Acc:J4U7B4]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1601		175860.14	4.5561

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_TasahiiCBS2479	8834	1186058	523	8311

• Gene Location: location=JH977603:1300376-1305933(+)

Full Sequence      

MKWLAGAAAFAAVAAAAPQSSVPFSVGVETAWSAPPLLLEILLSGHADISETAYDESASS
YFPLLRLITSHIVGLRKTPESQYEHALQLMGSHHICSGDESTFNLALSLHTTAPRIEAAF
AQYAKLDEAALGVTDCAAWVEFQGKAYCDAEKLRAAVERGIDGNSTEVPIRTLPFDHVSG
AEPKAVFYYSPNPDALPLLNYLDQHTKSVPEFAYVVRYRPTGERTRKTLLSGYGVEMALK
KTDYLVVDDRARAKGGKQVVFENKQAATGVGVNAITVIKNAKNPLKAFTDLSQNLPKYTA
ALARKVSAPDSIKKRARITGHEYPVSPLFLLNGKVIKEADLTGRLLNAIRSERPYINTLL
DIGFTPKEAVELMSDEVIGTAQASTSPSEGLVDASDREEGGNVIGWMYDIENDAHSVTNS
QYMRPVYPGQLHLVRRNALNAILAVDLSKKIGLGTVANEALGFVRRGVPVRFGVVPVGDN
TAGKIFFYINEKLGREAANDYIKTLDTITDGGKVTDVAVETAYESITESAVGEWPPFDAV
LKSKLVTKRSQRAQDWLERVGITPSESTGGTLMVNGKPIAIGVGWTRALQNELISMTQML
QQLIVGGAVGEDSDLSTVFYDLPSTLKRKAKYIVPKQGEKLVVYNLADVFNGTADVLAEN
FVYPGESSCGSGELTSVGDEAVPITTWIVGDLDSNAGLTLARNALEHLQTEGCRSRLGFV
NVQPSGGSGLFSSLLFELISTGQLNTIKPSQLIEFIDELNSRETNVDQLTLDEIQAGQAP
QELDTEGKDEPLNAFTGAGWTVGVTAEAGKFWSVGNTVAHRLGVNGTQPYVLVNGRLIGP
IDEPDVLRPKAFGLLEDFEGKKRVDPVAQLVASMSIGDGKDIPRSIQIGDFENALYHFGV
IVDPISETAQRWSGLLELLSSLDRVAISVHFDVDPRPKEIKVKRFYRESLRAQLSFDVDG
NEVSPDVRFVDLPSSPIYTLGMSVPQAWIISPTDSPLDLDNLMLGQLGEPTHVLFDLKQL
VMDGHAREGVSTPPTGLQLQLRAQGEDEVVSDTQVMTNLGYLQFKAPPGVYDLTIRPGRG
EEVYELQSAGNEGWESPSVQDAGNIVTLTSLEGNTIYPRFTRKEGMETADVLEVDEPAGW
LSKIKSAVGLAPVKKSRHADINIFTVASGLLYERFASIMMLSVMKHTKSSVKFWFIENFL
SPTFLKFLPQMAEHYGFEYELVTYKWPAWLRAQSEKQRIIWAYKILFLDVLFPMDLDKVI
FVDADQIVRTDMKELVDLDLEGHVYAYPPMGDDREEMEGFRFWKQGYWKNELRGNPYHIS
ALYVVDLQRFRAQTAGDRLRGMYHALSADPNSLANLDQDLPNSMQQQIPIFTLDKDWLWC
QTWCSDESLKTAKTIDLCQNPLTKEPKLSRARQIPEWDSYDREIAEFAASLGASELGGSV
DELAGTLEKAQGLKDSDAPSSSDDAATDSAEEEALKEEIAHENHVQAEQDEALEEILADA
DAPVSYESDRPESKRKTGPADSQKGGVKADSKADSKDPRQHPADGKAAVRDSVNEEEVEV
LVTYSDEPGAVWEPALKRDNTEPDAEKEEGEQTEEVVHDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1161	1408	6.2e-128	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1161	1427	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	8.95e-178	1161	1408	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
132996	Glyco_transf_8	4.49e-58	1161	1408	1	246	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
408201	Thioredoxin_14	9.30e-56	392	633	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	1.78e-50	1023	1133	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAW41515.2|GT24	0.0	12	1444	11	1555
KGB74213.2|GT24	0.0	15	1444	14	1555
AUB22656.1|GT24	0.0	12	1444	11	1555
AFR93153.2|GT24	0.0	12	1444	11	1555
UOH81582.1|GT24	0.0	12	1444	11	1558

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5NV4_A	1.12e-237	20	1428	7	1448	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5MU1_A	4.31e-237	20	1428	7	1448	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TRT_A	2.32e-236	20	1428	7	1448	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	4.54e-201	21	1370	1	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	1.77e-184	20	1428	7	1214	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09332|UGGG_DROME	8.76e-182	19	1424	25	1504	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q09140|UGGG_SCHPO	1.44e-181	23	1425	21	1422	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q9JLA3|UGGG1_RAT	9.45e-167	17	1426	40	1522	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	3.59e-165	21	1426	44	1522	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q6P5E4|UGGG1_MOUSE	8.80e-165	17	1426	40	1522	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000268	0.999714	CS pos: 16-17. Pr: 0.9701

TMHMM  Annotations     

There is no transmembrane helices in EJT46175.1.