CAZyme Information: EHY58502.1

Basic Information

• Species: Exophiala dermatitidis
• Lineage: Ascomycota; Eurotiomycetes; ; Herpotrichiellaceae; Exophiala; Exophiala dermatitidis
• CAZyme ID: EHY58502.1
• CAZy Family: GT2
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1517		169757.87	4.9130

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_EdermatitidisNIHUT8656	9356	858893	71	9285

• Gene Location: location=JH226134:2655992-2660599(+)

Full Sequence      

MRESTLPWRVTMLALWQFILVVASAASSSPSINVALETSFAAGPYLVELLETAALENSSS
YFPLLDRIAEGVFAELQTEQELYNKFIDVLQHDGHISSPESLSSFKFALALHAAAPRVEA
HFQYYNSSVQPHFMAAQDAVCPVWVYLDGQQYCSPELDRAQQSISLPEAEEMLPFDRVLG
SGLDGPASILYADITHPLFGQFHNVVSQTAREGTSTYRVRYRPAIGTARKPLFVSGYGVE
LVLKRTDYIVIDDRDASATEEAPDADQKALEQPKDDELKDLKPLTTSEVVNLGLNAASFI
ASSSDPLRSLKDITSDFPRYSSLLAATNASKAFISEHRSNRERFLPSGYNVFWINGVQIE
PRQINAYSLLDHLRRERRIIADLKGIGLTSTEAISLLSSEVIAEAQANEMPQRFDWRDDI
EGDTVLMWLNNLEQDKRYASWPSALRNLFQRVYPGQLPQLRRDIHNLIVPIDMGNVKDIE
LAATSLQGLVRRQVPIRVAIVPTGSGYLADVYAKLSYHILDKYGLAPLMGFLSEVSGSRK
LPANAEKSFQNAVKGLSPREGKQELSYNEVLNSEDLLKRLQARNAYLTRLALNSTDPPFI
IDGVVLPRTDNWFETMSSRLFADLQLLQQAVYQGAVPDDSWIPEFFLLEAFPRRNEIVFP
HDPKDVRIVDVSQLVSDFADEFDNLPRIPGSEAGLLSDRAHLFVVTDVNSEEGRTFLREV
LGFRHQHPEADVLILHNPPSESSPSGLECAIHALVGETGRDITPEQLQQILDGHSNQADG
EQKAHECGSYWSSKAKLTQSLGVTPGETGLWLNGRVLGPTHHALTAEDMEALLGFERRER
IAPVTTAITALGLEDRFSNPLNLAKVTSLVARSLKSDLPEGLRESAPLIRMDRFKIWNDT
HTSIKVANSEDPSIQAVAVVDPASEVVQQWVPILETLSKLHGVSVQIFLNPKDRLSELPV
KRFYRQVISSEPDFDENGSLTTPTASFSGVPRDTLFNLGMIVPPSWLVAPKKCVHDLDNI
RLSNLADGENIDALYELEYILIEGHARDVTVGPPPRGVQLLLGTEQEPHFTDTIVMANLG
YFQFKANPGYWQISLKPGRSSKIFHIDSIGPNGYAAQPGDETNSVALLSFQGVTLFPRLS
RKPGMEEEDVLEPPTSGIGGAMEYISKGASFASSALSSLGLKKGGASSSTNAEINIFSVA
SGHLYERMLNIMMVSVMKHTKHTVKFWFIEQFLSPSFKSTVPILAQHYGFDYEMVTYKWP
HWLRGQKEKQREIWGYKILFLDVLFPLDLDKVIFVDADQIVRTDLIELVNLDLKGAPYGF
TPMCDSRTEMEGFRFWKQGYWQTYLKGKPYHISALYVVDLKRFRQLAAGDRLRQQYQALS
ADPNSLSNLDQDLPNHMQHSLPIYSLSQEWLWCETWCSDESLAQAKTIDLCNNPLTKEPK
LDRARRQVPEWTEYDEEIAAVIKAAKAKDKAREDQSKETLSSSSSSRLLEQGDDDDDGEQ
DQKNIIDGEGERPRDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1194	1441	2e-135	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1194	1458	1	265	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	0.0	1194	1441	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408201	Thioredoxin_14	2.33e-84	414	658	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	1.05e-65	1043	1152	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.
408199	Thioredoxin_12	2.75e-59	44	224	3	179	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAK39488.1|GT24	0.0	21	1479	21	1476
CAS97678.1|GT24	0.0	21	1479	21	1476
BCR94558.1|GT24	0.0	21	1467	21	1461
BCS07152.1|GT24	0.0	21	1467	21	1461
UDD64319.1|GT24	0.0	14	1462	9	1456

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	27	1463	6	1449	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	27	1463	6	1449	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	28	1152	2	1124	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	27	1463	6	1449	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	27	1463	6	1215	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	5.70e-278	16	1479	6	1443	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	4.49e-227	26	1462	24	1508	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	1.42e-217	25	1458	40	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9NYU2|UGGG1_HUMAN	4.25e-217	10	1458	29	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q9JLA3|UGGG1_RAT	6.08e-214	2	1458	17	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000324	0.999666	CS pos: 25-26. Pr: 0.9772

TMHMM  Annotations     

There is no transmembrane helices in EHY58502.1.