CAZyme Information: EEU48866.1

Basic Information

• Species: Fusarium vanettenii
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium vanettenii
• CAZyme ID: EEU48866.1
• CAZy Family: PL1
• CAZyme Description: Beta-mannosidase B [Source:UniProtKB/TrEMBL;Acc:C7YIU3]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
853		97466.75	5.1144

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Fvanettenii77134	15708	660122	0	15708

• Gene Location: location=GG698896:3819346-3821959(-)

Full Sequence      

MGPRTVTPIDQNWHFKQAKDDDASYLSVAQFPTNVHLDLLHHGKIPDPFIGKNENEVQWI
GETDWVYKTTFASPKVADGAKAVLVFDGLDTFATVKLNGTKILETDNMFTSDRVDVTEIL
KSDGENELVITFGSAYLKGWDLVDEHPEHKYRCWNGDFSRLPVRKAQYHWGWDWGPALLT
CGPWKPINLEVYESRIYDLHAQVEVEESLKTATVVVNADVEGKASQAQLVVTLDGKEVAS
TTTDVKDSKVSTTFHIQDPALWYPIRYGKQPLYTIQATLLENETKVDTTDKKIGLRKVEL
VQHELEGQPGTSFFFRVNNIPVFCGGSNWIPADNFLPRISKKRYYDWVNLVAESNQFMIR
VWGGGIFESDFFYDACDEAGILVWQDFLFGCGNYPCWSELRKSIDREARENIKRLRHHPS
IVIWAGNNEDYQIAESEKLTYDPKDKDPESWLKTDFPARYIYEKILPEACSEVNPGSIYH
FGSPYGGHDTTDPTVGDIHQWNIWHGAQRRYQDFDKLVGRFVSEFGMEAFPNIKTIDSYL
PKGKDDPERFYSSSTIDFHNKADGQIRRLVIYLAENFRYTQEPLEYYVYCTQLMQAECLA
SAYRLWKRQWQGPNKEYCSGALVWQINDCWPVTSWSICDYYLRPKHAYYTIKREMAPVTI
GMTRTEHQHPRSKHTRVDIDTKIQVEIWGSNLELEDVTADCVVKAWDIETGKETYSKTVS
PSFILPSNRSTEIATLEVPVETPNAGLEARTVVASYLYQDGKQIARYVNWPEPHKYSPLQ
KPKNLRVELVDGASAVEVSAEVPVKGLALECEDDGVRFADNLVDLVPGEIVRIGVKGAVE
ATAITTQYLGLHY

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	5	743	7.6e-96	0.7007978723404256

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	4.04e-88	1	652	7	627	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	2.09e-16	684	776	2	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
236657	PRK10150	2.00e-12	67	429	69	417	beta-D-glucuronidase; Provisional
395572	Glyco_hydro_2	5.42e-11	195	296	2	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
407625	Ig_mannosidase	5.57e-05	786	843	7	64	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UPK99083.1|GH2	0.0	1	853	1	853
QKD46795.1|GH2	0.0	1	851	1	851
QPC75292.1|GH2	0.0	1	851	1	851
CEF72811.1|GH2	0.0	1	853	1	853
QPC66527.1|GH2	0.0	1	851	1	851

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	2.90e-146	1	662	21	660	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	2.97e-118	6	833	8	814	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	3.11e-118	6	833	10	816	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	3.19e-118	6	833	10	816	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	8.31e-118	6	833	8	814	Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|I2C092|MANBB_THETO	0.0	1	851	1	854	Beta-mannosidase B OS=Thermothelomyces thermophilus OX=78579 GN=man9 PE=1 SV=1
sp|Q5B7W2|MANBB_EMENI	1.38e-275	1	850	1	842	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2
sp|Q0CCA0|MANBB_ASPTN	1.11e-274	13	850	13	842	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
sp|A1CGA8|MANBB_ASPCL	1.15e-269	8	837	8	830	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
sp|A1D911|MANBB_NEOFI	6.57e-269	8	837	8	830	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000078	0.000000

TMHMM  Annotations     

There is no transmembrane helices in EEU48866.1.