CAZyme Information: EEU33801.1

Basic Information

• Species: Fusarium vanettenii
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium vanettenii
• CAZyme ID: EEU33801.1
• CAZy Family: AA11
• CAZyme Description: Beta-mannosidase [Source:UniProtKB/TrEMBL;Acc:C7ZQC0]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
840	GG698987|CGC1	95946.96	5.1925

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Fvanettenii77134	15708	660122	0	15708

• Gene Location: location=GG698987:35828-38657(-)

Full Sequence      

MLAAAKRTYLESGWSFKSGQDGDEDWKPVARVPTVVHMDLMDNGIISDPFLDMNEIDAEW
VGETSWSYRTTFASPAKEGSSVYLLFDGLDTFAQVKLNESVILESSNMFLSHRVDVTNLL
KKDNFLTINFDSALLRGREIKGKLSDHQFVLFNGEAGRLTVRKAQYHWGWDWGPKLMTAG
PWRPVRLEVSYAHIDDVFVKYELSDDLKMVEGSVDIEVDGGFDTACLSVRFEDNVVFSSE
ASSPSTGRASIPFNIKDPKPWYPAGYGNQHMYEVSVDIIKHGQVLDSWTKKTGFRRSELI
QRDDSHGQSFFFRVNGIDIFCGGSCWIPADSLLPKITPDRYRQWLQLMVEGNQIMTRVWG
GGIYEEDAFYDACDELGILVWQDFMFACGNYPVWPEFIDSIRHEAKQNVQRLRHHPSVVI
YAGNNEDYQVQEASGLDYDWEDKDPQSWLKTNYPARYYYEHLLPEVVAKESQGVIYWPAS
PFSSGKLTNDKTVGDIHQWNGKFPATKSVWHGTQEKYQTFHNLGGRFNSEFGMEAFPVLS
TIQHFITDTAEYFQQSQTMDYHNKADGHERRLATYLVENFRPREDLESYVYLTQLCQSEA
LTYAYRGWRRQWGDDRRCGGVLVWQLNDCWPGISWAIVDYFLRKKPAFYAISRALEPLAV
AVERDHHDWSVCHARPAKKSPFSVWIVNSQREVKGIVEVRFVSVKTGNDIKDKILRENVT
ITGNGTTEVLSGEIDNVKEEPHVIAVRLLVDGVCISRSVDWPQPMKYLDLSERNVKVEFF
YAQYVITAQRPTKGVVLEEVDGVTLSDNCVDVVPGDEVRIAISGSVSAAQSPKFKYLGQP

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	5	672	2.6e-97	0.6303191489361702

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	1.85e-78	44	664	45	639	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	5.25e-14	680	767	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
395572	Glyco_hydro_2	4.24e-13	192	295	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
236673	ebgA	8.22e-11	68	428	113	452	cryptic beta-D-galactosidase subunit alpha; Reviewed
397119	Glyco_hydro_2_C	0.002	369	428	64	137	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UPK91989.1|GH2	0.0	1	840	1	832
QKD57951.1|GH2	0.0	1	837	1	832
CCT70824.1|GH2	0.0	1	837	1	832
CEI68069.1|GH2	0.0	1	838	1	832
QGI83791.1|GH2	0.0	1	837	1	832

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	1.85e-147	2	663	21	661	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	6.96e-114	10	835	11	822	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	7.29e-114	10	835	13	824	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	7.46e-114	10	835	13	824	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	1.94e-113	10	835	11	822	Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|B8NW36|MANBB_ASPFN	0.0	2	839	1	844	Beta-mannosidase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndB PE=3 SV=1
sp|B0YBU9|MANBB_ASPFC	0.0	10	839	9	845	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
sp|Q2TXB7|MANBB_ASPOR	0.0	2	839	1	844	Beta-mannosidase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndB PE=3 SV=3
sp|Q4WAH4|MANBB_ASPFU	0.0	10	839	9	845	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
sp|Q0CCA0|MANBB_ASPTN	0.0	2	839	1	843	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000070	0.000000

TMHMM  Annotations     

There is no transmembrane helices in EEU33801.1.