CAZyme Information: EEQ92378.1

Basic Information

• Species: Blastomyces dermatitidis
• Lineage: Ascomycota; Eurotiomycetes; ; Ajellomycetaceae; Blastomyces; Blastomyces dermatitidis
• CAZyme ID: EEQ92378.1
• CAZy Family: GH55
• CAZyme Description: endo-1,3(4)-beta-glucanase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
899		97529.00	4.9829

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_BdermatitidisER-3	10081	559297	326	9755

• Gene Location: location=EQ999981:697136-700278(-)

Full Sequence      

MTPSMPLWPAAILIMAMLHFPAAHALPCRSNYQCVVDVDQADNLSFSPITQVPTATVVAH
GDTTGIYFTQMESDVRSYSQPFPTDISYIVFEDHIGLLHPGVPHLDSVPSAVLSSNSNCY
YKGHLCPGLPHVEETRIANWLLSPSTASLNADISIGITIPPPPPFLESSTASCSPPYPIT
SSKSSSLAPIVPSPSSSTPTATPSPKLPSMSGQNIFEPISRDQIPQNIPSRDDHPLKRDN
ISDTPGPVSTNKFYANFFIGSQANYGFVQPYAVGWAKGTGSVKSYGLTISHTEANQLAFG
PKNPKIPGEPVQFYINPIGIQSMILSAAEIGSSTVLTVSKPLAFSAEIILRPNRDSDSNI
TFPLVQGMGYVTGVYKDLQPAIQSSVFFRQLESAPSPKLGIFKYKVILEDNTNWLIYVTP
ADGADPRLKLENNGLINGPAGFSGTIQVAKNPNGGPGETIFDGSAGVYPMAGNITGSVVA
QDATGTYRLAWEKGGKDVDSTPLLMFALPHHVQSFDSETQGRKVDLVLRTTTKGIATAVV
ADLWTMVETDLPIDMGFAPWNPAGTSATLSRPAKQMVKDIAPNELSQDIHQQTNLNSMYF
SGKAFGKFAMVVYAVRELAQDVPLSESAFDTLKDAFATFVNNEQQWPLVYDNVWKGIVSS
GTYETGDPGLDFGNTFYNDHHFHYGYFILSAAIIGSLDPSWIDANKAWVNALVRDAANSV
SDDPFVSVFPEDLTDVRQGVKDPSMEARGNMMLAILARSLNNYFLMKSDNVNQPPNFIGN
KVTGILFENKADHTTYFGPNLEYIQGIHMLPIIPSSAYTRHKDFVREEWDAMFSDRASTP
ASKVQGGWQGVLYSNLALIDPDASWNFFAQEPFDLGKIDGGASRTWYLAYAAGLGGGPH

Enzyme Prediction     

EC	3.2.1.39:20

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	235	726	1.1e-127	0.6768488745980707

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
227785	Acf2	0.0	171	898	19	759	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
407548	Glyco_hydro81C	1.77e-180	568	888	1	349	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
397619	Glyco_hydro_81	1.21e-156	234	562	1	321	Glycosyl hydrolase family 81. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein ENGL1, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS67487.1|GH81	0.0	208	898	126	850
QSS57493.1|GH81	0.0	208	898	126	850
QSS66079.1|GH81	0.0	206	898	126	852
ABH10633.1|GH81	6.16e-313	210	898	1	718
UDD56325.1|GH81	4.65e-296	211	896	6	723

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4K3A_A	1.69e-85	212	892	5	716	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K3A_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
5XBZ_A	2.77e-85	212	892	30	741	Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XBZ_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XC2_A Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_C Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_D Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei]
4K35_A	4.03e-82	212	892	5	716	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K35_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
6FOP_A	4.78e-09	583	871	325	637	Chain A, Glycoside hydrolase family 81 [Acetivibrio thermocellus ATCC 27405]
5UPI_A	9.91e-08	678	884	439	674	Chain A, BH0236 protein [Halalkalibacterium halodurans C-125],5UPM_A Chain A, BH0236 protein [Halalkalibacterium halodurans C-125],5UPN_A Chain A, BH0236 protein [Halalkalibacterium halodurans C-125],5UPO_A Chain A, BH0236 protein [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4AZ24|ENG1_ARTBC	1.01e-314	81	893	74	901	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
sp|Q09850|ENG2_SCHPO	1.20e-139	214	898	2	706	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1
sp|Q9UT45|ENG1_SCHPO	1.48e-127	214	894	46	737	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1
sp|Q5AIR7|ENG1_CANAL	1.51e-126	220	893	442	1140	Endo-1,3(4)-beta-glucanase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ENG1 PE=1 SV=1
sp|P53753|ENG1_YEAST	9.19e-124	214	894	401	1112	Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000211	0.999769	CS pos: 25-26. Pr: 0.9754

TMHMM  Annotations     

There is no transmembrane helices in EEQ92378.1.