CAZyme Information: EDK45861.1

Basic Information

• Species: Lodderomyces elongisporus
• Lineage: Ascomycota; Saccharomycetes; ; Debaryomycetaceae; Lodderomyces; Lodderomyces elongisporus
• CAZyme ID: EDK45861.1
• CAZy Family: GT34
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
283		32121.86	4.3514

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_LelongisporusNRRLYB-4239	5908	379508	109	5799

• Gene Location: location=CH981528:987917-988768(-)

Full Sequence      

MRLNFVISLLYIIGFLSAEANADKINVNCDMNTDNGIGIDVDIDVDIDVDIGVDIDSNEG
SSISKEYYHENYNLINDSVFESLTNGTEFLSIKYSHDGINFSSKGLQMTIKKPFDNPSLV
STEYIMYGKVEAEIKGSHGSGLVSSLYLQSKDLDEIDIAEILGSDPYRYQTNFFIKGNTT
TYDRGDFHNLPIHLSHDQFYKYGCEWTFEKIVWFLNDVPVRELIRGNKHGMPTSPMRVIF
SLWAGEDTQNKDTIAWSGGLTDYSRGPFTMTVRNLRVENYIDE

Enzyme Prediction     

EC	2.4.1.-:3	3.2.1.39:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	104	259	2e-50	0.9873417721518988

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185692	GH16_fungal_CRH1_transglycosylase	1.14e-79	93	280	13	202	glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
395585	Glyco_hydro_16	1.16e-42	94	258	1	168	Glycosyl hydrolases family 16.
185684	GH16_lichenase	1.63e-25	84	276	20	209	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
185683	Glyco_hydrolase_16	4.07e-22	94	277	27	209	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
185685	GH16_XET	1.43e-19	94	278	14	201	Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCG24345.1|GH16_18	1.79e-83	71	280	71	279
CCE40070.1|GH16_18	1.05e-81	78	280	79	280
AOW29885.1|GH16_18	3.28e-78	68	280	46	261
AAV02850.1|GH16_18	4.45e-77	68	280	68	283
CAX41820.1|GH16_18	6.11e-76	79	280	70	269

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6IBU_A	7.03e-45	104	279	47	223	Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBU_B Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBW_A Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293],6IBW_B Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293]
2UWC_A	2.58e-11	127	243	61	185	Crystal structure of Nasturtium xyloglucan hydrolase isoform NXG2 [Tropaeolum majus],2UWC_B Crystal structure of Nasturtium xyloglucan hydrolase isoform NXG2 [Tropaeolum majus]
2UWB_A	1.15e-10	127	243	62	181	Crystal structure of the Nasturtium seedling mutant xyloglucanase isoform NXG1-delta-YNIIG [Tropaeolum majus],2UWB_B Crystal structure of the Nasturtium seedling mutant xyloglucanase isoform NXG1-delta-YNIIG [Tropaeolum majus]
2UWA_A	1.22e-10	127	243	64	188	Crystal structure of the Nasturtium seedling xyloglucanase isoform NXG1 [Tropaeolum majus],2UWA_B Crystal structure of the Nasturtium seedling xyloglucanase isoform NXG1 [Tropaeolum majus],2UWA_C Crystal structure of the Nasturtium seedling xyloglucanase isoform NXG1 [Tropaeolum majus]
2VH9_A	1.37e-10	127	243	85	204	CRYSTAL STRUCTURE OF NXG1-DELTAYNIIG IN COMPLEX WITH XLLG, A XYLOGLUCAN DERIVED OLIGOSACCHARIDE [Tropaeolum majus],2VH9_B CRYSTAL STRUCTURE OF NXG1-DELTAYNIIG IN COMPLEX WITH XLLG, A XYLOGLUCAN DERIVED OLIGOSACCHARIDE [Tropaeolum majus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5AK54|CRH12_CANAL	5.82e-79	68	280	46	261	Extracellular glycosidase CRH12 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=CRH12 PE=1 SV=1
sp|Q5AFA2|CRH11_CANAL	1.95e-55	75	280	38	242	Extracellular glycosidase CRH11 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=CRH11 PE=1 SV=1
sp|P53301|CRH1_YEAST	5.23e-51	89	280	69	257	Probable glycosidase CRH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CRH1 PE=1 SV=1
sp|D4ALQ5|CRH11_ARTBC	1.74e-43	101	280	60	238	Probable extracellular glycosidase ARB_05253 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_05253 PE=1 SV=2
sp|Q8J0P4|CRF1_ASPFU	1.34e-42	104	279	68	244	Probable glycosidase crf1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=crf1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000220	0.999757	CS pos: 22-23. Pr: 0.9737

TMHMM  Annotations     

There is no transmembrane helices in EDK45861.1.