CAZyme Information: EAW20969.1

Basic Information

• Species: Aspergillus fischeri
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus fischeri
• CAZyme ID: EAW20969.1
• CAZy Family: GH43
• CAZyme Description: ER glycosyl hydrolase (Edem), putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1078		119653.02	6.1665

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AfischeriNRRL181	10668	331117	278	10390

• Gene Location: location=DS027692:870751-874223(+)

Full Sequence      

MLELTDVRPSVSMHDMGLTPVAMARHGSGKETEHMFYHGFENYMKYAFPEDELRPLSCRP
LVRDRDNPSNAELNDVLGNYSLTLIDSLSSLAILSSSPDEGERAWNHFQDGVRDFVKLYG
DGSDGPAGQGERARGFDLDSKVQVFETVIRGLGGLLSAHLFAVGDLPITRYTPPEPEATF
AKAWNKSAFPGHDHGIKWTNGFVYDGQLLRLAADLAKRLLPAFYTDTGLPYPRVNLRYGV
QRRPFYANSPLNADKQCHGPDREVCREDRQRAPSETTETCSAGAGSLVLEFTVLSRLTGD
GRYEELAKRAFWAVWTRRSDIGLIGSGIDAESGRWVHSYTGIGAGIDSFFEYAFKSYVLL
SSGQRSSHDPRSPWQVLDGYFPPLTEYEHSAEAFLRVWEEAHAAIKRHLYRGEGYQHPHV
IQGDIFTGATRAFWIDSLSAFYPGLLSIAGELDEAIAIHLLTTAVWTRFSGLPERWNVAT
GNIEGELAWYGGRPEFIESTYYIYRATKDPWYLHVGEMVLRDLKRRCWTKCGWAGLQDVR
NGELNDRMESFFLGETAKYLFLLYHPDHPLNNMDRPFVFSTEGHPLIIPTRTSSSTHQPR
KQAQHEEPVNLPVCQLAPEPPTFGLSSTAARPDVFHAATLARLHLMPSRGPTEGPILDYA
HDHPSVTVSDLSSPTNYTYYPWTLPPELVPFNATSSPMTSRPTLDISFPAIPGMVMGPGS
IERVRDGIFIKNIGGLRLSMVQDVPSLDVTGKASQDDFRVQVINNVPLGKDEKVYLSREI
TFDILDPTDPNFTRMRDSAMIDIVIDVMPELLRRGNDSNGSRERGAAERSENHVIQKNAS
VDDKIGSVDPSTSGMKNVFSSLMDTVSALLRDEDPGLTTQSPSRKSSIVRLSLPAAVSSG
AGSAPVPEVEDASIVSISGEPSKSRLSWSTIYFADEICDRRILREIAQSHQVLVIKRGGC
SFSQKLRNIAAYPPSRHALKLVIVVDYDENTFAESSTSTPPPSAGLAAIRAEPFLIRPLL
DEPQMTAGGLPRRHPISMVMVGGGEETYELLLRATGVGIKRRYSVRSQGIPINNLYIV

Enzyme Prediction     

No EC number prediction in EAW20969.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH47	35	587	5.9e-144	0.9955156950672646

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396217	Glyco_hydro_47	4.24e-132	35	588	1	453	Glycosyl hydrolase family 47. Members of this family are alpha-mannosidases that catalyze the hydrolysis of the terminal 1,2-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2).
240427	PTZ00470	4.70e-56	30	589	74	519	glycoside hydrolase family 47 protein; Provisional
239038	PA_C_RZF_like	0.004	947	990	65	103	PA_C-RZF_ like: Protease-associated (PA) domain C_RZF-like. This group includes various PA domain-containing proteins similar to C-RZF (chicken embryo RING zinc finger) protein. These proteins contain a C3H2C3 RING finger. C-RZF is expressed in embryo cells and is restricted mainly to brain and heart, it is localized to both the nucleus and endosomes. Additional C3H2C3 RING finger proteins belonging to this group, include Arabidopsis ReMembR-H2 protein and mouse sperizin. ReMembR-H2 is likely to be an integral membrane protein, and to traffic through the endosomal pathway. Sperizin is expressed in haploid germ cells and localized in the cytoplasm, it may participate in spermatogenesis. The significance of the PA domain to these proteins has not been ascertained. It may be a protein-protein interaction domain. At peptidase active sites, the PA domain may participate in substrate binding and/or promoting conformational changes, which influence the stability and accessibility of the site to substrate.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABF69939.1|GH47	0.0	1	1078	1	1083
BCS05200.1|GH47	0.0	30	1078	45	1096
BCS16758.1|GH47	0.0	30	1078	45	1096
QMW34129.1|GH47	0.0	30	1078	45	1090
QMW46185.1|GH47	0.0	30	1078	45	1090

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5KIJ_A	4.18e-32	35	588	12	451	Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase and Man9GlcNAc2-PA complex [Homo sapiens]
1FMI_A	4.58e-32	35	588	17	456	Crystal Structure Of Human Class I Alpha1,2-Mannosidase [Homo sapiens]
1FO2_A	4.72e-32	35	588	17	456	Crystal Structure Of Human Class I Alpha1,2-Mannosidase In Complex With 1-Deoxymannojirimycin [Homo sapiens],1FO3_A Crystal Structure Of Human Class I Alpha1,2-Mannosidase In Complex With Kifunensine [Homo sapiens]
1X9D_A	1.30e-31	35	588	95	534	Crystal Structure Of Human Class I alpha-1,2-Mannosidase In Complex With Thio-Disaccharide Substrate Analogue [Homo sapiens]
5KK7_A	1.92e-31	35	588	12	451	Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase T688A mutant and Thio-disaccharide substrate analog complex [Homo sapiens],5KK7_B Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase T688A mutant and Thio-disaccharide substrate analog complex [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O94726|MNL1_SCHPO	3.44e-121	35	598	42	487	ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mnl1 PE=3 SV=2
sp|Q92611|EDEM1_HUMAN	2.10e-108	35	588	137	586	ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Homo sapiens OX=9606 GN=EDEM1 PE=1 SV=1
sp|Q925U4|EDEM1_MOUSE	2.55e-108	35	591	132	584	ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Mus musculus OX=10090 GN=Edem1 PE=1 SV=1
sp|P38888|MNL1_YEAST	7.04e-104	30	615	37	529	ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MNL1 PE=1 SV=1
sp|Q9SXC9|MNS5_ARATH	7.62e-88	30	588	39	478	Alpha-mannosidase I MNS5 OS=Arabidopsis thaliana OX=3702 GN=MNS5 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000074	0.000001

TMHMM  Annotations     

There is no transmembrane helices in EAW20969.1.