CAZyme Information: EAW17381.1

Basic Information

• Species: Aspergillus fischeri
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus fischeri
• CAZyme ID: EAW17381.1
• CAZy Family: GH10
• CAZyme Description: endo-1,3(4)-beta-glucanase, putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
468	DS027696|CGC5	52796.17	4.6804

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AfischeriNRRL181	10668	331117	278	10390

• Gene Location: location=DS027696:231678-233084(-)

Full Sequence      

MKLFLFSLLPLLFATTILSYPAPSNSSTISPRLPIDFYKRQCDCYVVSGSETVYFENYAF
WDFRKIPLPRYDSNGSKGRANRRSYTRSRADRTDNGEGDYEEENVGIEENGDNKENFNDE
EDTDDEENTEDEDNADDDENDHDGMKAVGEGPDLGTLLFSQTAFARDWKPQKWHRKSNKY
GPLPIINSPENIFFASNTLYTNDSESTHLVLRTTRFPNHTATAELEYHLRNIFHCSLRVR
MRVMTVGSVNREPMLYGRPVPKGNPTNQSVVPGGACAGIFTYRSSTCESDIEILTSDPTN
IVRYANQPDYDPITDAMIPGAASIGTLSQPWTNPTTHRVDWLHNITKWYANGEIKASNTY
HVPDQPSMMAINLWSNGGEWTGEMPVGQSAYIGIEWIEVAYNTSTEVNGAPSDTVPFPSH
KHRPFARSPEVGPESDHETSAGEVEFIQRKDAARQCLRPCFLDDVRGH

Enzyme Prediction     

No EC number prediction in EAW17381.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	166	399	7.8e-75	0.9907407407407407

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185683	Glyco_hydrolase_16	8.28e-38	159	399	3	209	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCS29547.1|GH16_22	3.12e-97	14	466	18	383
QQK40468.1|GH16_22	1.90e-91	31	465	29	449
CAP95204.1|GH16_22	3.01e-91	31	411	69	422
BCR99502.1|GH16_22	8.25e-89	14	463	6	405
BCS11802.1|GH16_22	8.25e-89	14	463	6	405

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1GBG_A	9.57e-08	250	397	64	209	Bacillus Licheniformis Beta-Glucanase [Bacillus licheniformis]
3O5S_A	1.26e-07	274	397	110	233	Crystal Structure of the endo-beta-1,3-1,4 glucanase from Bacillus subtilis (strain 168) [Bacillus subtilis]
1AXK_A	3.91e-07	336	406	88	160	Engineered Bacillus Bifunctional Enzyme Gluxyn-1 [Bacillus subtilis],1AXK_B Engineered Bacillus Bifunctional Enzyme Gluxyn-1 [Bacillus subtilis]
1CPN_A	1.77e-06	336	404	88	158	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1MAC_A	3.35e-06	336	402	144	212	Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans],1MAC_B Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P04957|GUB_BACSU	6.73e-07	274	397	114	237	Beta-glucanase OS=Bacillus subtilis (strain 168) OX=224308 GN=bglS PE=1 SV=2
sp|P27051|GUB_BACLI	2.97e-06	250	382	93	223	Beta-glucanase OS=Bacillus licheniformis OX=1402 GN=bg1 PE=1 SV=1
sp|P45797|GUB_PAEPO	6.86e-06	274	402	110	238	Beta-glucanase OS=Paenibacillus polymyxa OX=1406 GN=gluB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000256	0.999715	CS pos: 19-20. Pr: 0.9824

TMHMM  Annotations     

There is no transmembrane helices in EAW17381.1.