CAZyme Information: EAW16650.1

Basic Information

• Species: Aspergillus fischeri
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus fischeri
• CAZyme ID: EAW16650.1
• CAZy Family: AA7
• CAZyme Description: beta-1,4-mannosyltransferase (Alg1), putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
461	DS027698|CGC14	51257.36	8.9631

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AfischeriNRRL181	10668	331117	278	10390

• Gene Location: location=DS027698:3812935-3814452(+)

Full Sequence      

MIESLISVAFYISTAVTLLLFLLPSRYDPRRFDRLKKDASIAPKTTTQILVLGDIGRSPR
MQYHALSIARGGGQVDLIGYNESEVHPDISSNPRISITALAPHPTFLQTSNKLLFLLFGP
LKVAFQIVCLWWALAYRTEPAQWLLVQNPPSIPTLAIASMASFLRHTKLIIDWHNFGYTI
LALKLGDRHPLVRFSKWYEKSFCRYATAHFCVTEAMASILKNHFGLTAPILPLHDRPASH
FQPIFDQSEQKSFLESLPETAPVKDLLQAGSLRVIVSSTSWTADEDFSLLIDALCRYSNL
ANTSKPALPAVLAIITGKGPQKEMYLKQISKLQEAGKLSKVTIRTTWLTTDDYARLLASA
SLGISLHTSSSGVDLPMKVVDMFGAGLPVLGWDRFQAWPELVTEGVNGMGFGSSGELLDH
LVDLFENPSKLEKIRTGARKESNRRWNDEWDPIAGKLLGLA

Enzyme Prediction     

EC	2.4.1.142:10	2.4.1.-:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT33	45	456	1e-162	0.9929411764705882

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340843	GT33_ALG1-like	0.0	43	458	2	411	chitobiosyldiphosphodolichol beta-mannosyltransferase and similar proteins. This family is most closely related to the GT33 family of glycosyltransferases. The yeast gene ALG1 has been shown to function as a mannosyltransferase that catalyzes the formation of dolichol pyrophosphate (Dol-PP)-GlcNAc2Man from GDP-Man and Dol-PP-Glc-NAc2, and participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. In humans ALG1 has been associated with the congenital disorders of glycosylation (CDG) designated as subtype CDG-Ik.
215155	PLN02275	1.50e-146	47	425	7	371	transferase, transferring glycosyl groups
223515	RfaB	8.07e-15	52	458	14	371	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
340831	GT4_PimA-like	1.55e-13	151	460	90	364	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.
340825	GT4_WbuB-like	1.37e-11	130	458	88	391	Escherichia coli WbuB and similar proteins. This family is most closely related to the GT1 family of glycosyltransferases. WbuB in E. coli is involved in the biosynthesis of the O26 O-antigen. It has been proposed to function as an N-acetyl-L-fucosamine (L-FucNAc) transferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAX31662.1|GT33	5.21e-314	1	461	1	505
ACQ04909.1|GT33	8.49e-265	76	461	42	450
BAE65834.1|GT33	8.82e-260	1	460	1	458
QRD93281.1|GT33	1.78e-259	1	460	1	458
QMW47865.1|GT33	1.78e-259	1	460	1	458

PDB Hits     

EAW16650.1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4AZD1|ALG1_ARTBC	2.34e-151	22	461	22	446	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01551 PE=3 SV=1
sp|Q6C3K2|ALG1_YARLI	3.84e-108	15	461	16	441	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=ALG1 PE=3 SV=1
sp|O13933|ALG1_SCHPO	1.48e-100	49	458	29	420	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=alg1 PE=3 SV=2
sp|Q8L7M0|TUN_ARATH	8.59e-92	49	458	9	455	UDP-glycosyltransferase TURAN OS=Arabidopsis thaliana OX=3702 GN=TUN PE=2 SV=1
sp|Q59Q79|ALG1_CANAL	4.55e-89	44	450	51	444	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ALG1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000081	0.000000

TMHMM  Annotations     

Start	End
5	27
113	135