Species | Chaetomium globosum | |||||||||||
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Lineage | Ascomycota; Sordariomycetes; ; Chaetomiaceae; Chaetomium; Chaetomium globosum | |||||||||||
CAZyme ID | EAQ86714.1 | |||||||||||
CAZy Family | GH11 | |||||||||||
CAZyme Description | Glucoamylase [Source:UniProtKB/TrEMBL;Acc:Q2GVN7] | |||||||||||
CAZyme Property |
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Genome Property |
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Gene Location |
EC | 3.2.1.3:94 | 3.2.1.3:94 | 3.2.1.1:13 |
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Family | Start | End | Evalue | family coverage |
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GH15 | 55 | 228 | 6.5e-38 | 0.45706371191135736 |
CBM20 | 490 | 557 | 2.9e-19 | 0.7 |
Cdd ID | Domain | E-Value | qStart | qEnd | sStart | sEnd | Domain Description |
---|---|---|---|---|---|---|---|
395586 | Glyco_hydro_15 | 1.33e-121 | 48 | 444 | 1 | 416 | Glycosyl hydrolases family 15. In higher organisms this family is represented by phosphorylase kinase subunits. |
99886 | CBM20_glucoamylase | 5.48e-35 | 484 | 594 | 1 | 106 | Glucoamylase (glucan1,4-alpha-glucosidase), C-terminal CBM20 (carbohydrate-binding module, family 20) domain. Glucoamylases are inverting, exo-acting starch hydrolases that hydrolyze starch and related polysaccharides by releasing the nonreducing end glucose. They are mainly active on alpha-1,4-glycosidic bonds but also have some activity towards 1,6-glycosidic bonds occurring in natural oligosaccharides. The ability of glucoamylases to cleave 1-6-glycosidic binds is called "debranching activity" and is of importance in industrial applications, where complete degradation of starch to glucose is needed. Most glucoamylases are multidomain proteins containing an N-terminal catalytic domain, a C-terminal CBM20 domain, and a highly O-glycosylated linker region that connects the two. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. |
395557 | CBM_20 | 8.65e-23 | 490 | 556 | 1 | 66 | Starch binding domain. |
119437 | CBM20 | 2.69e-17 | 492 | 557 | 2 | 66 | The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. |
215006 | CBM_2 | 3.72e-16 | 492 | 556 | 3 | 67 | Starch binding domain. |
Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End |
---|---|---|---|---|---|
1.45e-259 | 1 | 595 | 1 | 628 | |
1.45e-254 | 1 | 595 | 1 | 657 | |
8.30e-231 | 38 | 556 | 2 | 566 | |
2.73e-228 | 1 | 556 | 1 | 582 | |
2.73e-228 | 1 | 556 | 1 | 582 |
Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
---|---|---|---|---|---|---|
2.12e-195 | 38 | 555 | 1 | 562 | Chain A, GLUCOAMYLASE [Trichoderma reesei],2VN7_A Chain A, GLUCOAMYLASE [Trichoderma reesei] |
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3.05e-153 | 38 | 556 | 2 | 581 | Structure of the catalytic domain of Aspergillus niger Glucoamylase [Aspergillus niger] |
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2.12e-143 | 38 | 506 | 2 | 470 | Catalytic domain of glucoamylase from aspergillus niger complexed with tris and glycerol [Aspergillus niger] |
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7.05e-143 | 42 | 556 | 11 | 559 | Crystal structure of Penicillium oxalicum Glucoamylase [Penicillium oxalicum 114-2] |
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1.10e-141 | 37 | 556 | 29 | 573 | Chain A, Glucoamylase P [Amorphotheca resinae],6FHW_B Chain B, Glucoamylase P [Amorphotheca resinae] |
Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
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1.58e-221 | 1 | 555 | 1 | 591 | Glucoamylase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=gla-1 PE=1 SV=3 |
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4.73e-159 | 39 | 556 | 30 | 578 | Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2 |
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2.34e-152 | 36 | 556 | 24 | 605 | Glucoamylase OS=Aspergillus awamori OX=105351 GN=GLAA PE=1 SV=1 |
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2.34e-152 | 36 | 556 | 24 | 605 | Glucoamylase OS=Aspergillus niger OX=5061 GN=GLAA PE=1 SV=1 |
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8.26e-151 | 36 | 556 | 24 | 576 | Glucoamylase ARB_02327-1 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02327-1 PE=1 SV=1 |
Other | SP_Sec_SPI | CS Position |
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0.000408 | 0.999552 | CS pos: 20-21. Pr: 0.9798 |
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