CAZyme Information: CXQ87_001158-t45_1-p1

Basic Information

• Species: [Candida] duobushaemulonis
• Lineage: Ascomycota; Saccharomycetes; ; Debaryomycetaceae; Candida; [Candida] duobushaemulonis
• CAZyme ID: CXQ87_001158-t45_1-p1
• CAZy Family: GH18
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1355	PKFP01000008|CGC25	151268.07	5.2091

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CduobushaemulonisB09383	5329	N/A	156	5173

• Gene Location: location=PKFP01000008:2514997-2519555(-)

Full Sequence      

MLKRATKSLESPENTKPFRFDDIEDARSPRRPKAPRQKEKSETLPKKRRKKQVEDDDDYV
ESHSQHTLQLTDSERPGPVRRSSYSNRGKRVSSVGNGFVAKPHDEMSARDYFKVVDGSLP
GPSRMRQILVWCFKKKLQQDREKDGPETDTAKGIAKVIQSEILDDLVDKKIDTSWLSARR
FDTAELQGKRIIKSNPLNDANRESVEVFQQKLRELRQEKVLWQNAFDASVKPLEGLGVSI
EDEEGKYSREFKEYVKQKDPALGAVLQEEHLEALSQHVQQVEAGVSEKLETNMAQLFHTA
YQLSKSAELVGKIKNESLAPQNSGLDSLEELVQSRENKEIFTKLLFADNAFYDKDAQTLG
TLDWVPYHQYQRQPYVANGYLGSRIPNLGQGFAYDTLSNGTVVDPGTPDAELPSDLSNGW
PLFNRRYSGAFVAGFYNIQENTTMTNFPWLLQDGYESVVAAVPQWTTLTLSAKVGGKSYS
LDPSNDPAKFGEITNYAQNLSLATGVVTTSFTWLGSLQVTYSVLAHRSKISLGLVNLKVQ
NIGFNNITVDVLDSLDFETAQRARFRETSVDKSSKAIYMLYSPNDIDYVYGATYSAIRYD
KSEAHCIGAEFTTSHYSSGQRITFEVPAASSIEATKHVGVVTSDLDPNKFASFDAVLREA
RSVALEKESFDSLFESHASEWSKTVDDGLKVEFPDDPLLTLAARASIYHLNANTRDDASG
LTAAMGVTGLSSDSYGGMVFWDTDLWMMNGLLPFSPSHARSFVNYRLHTHEQARKNIESD
YAPKKDMHGAAFPWTSGRYGNCTSTGPCFDYEYHINMAVANSAWELFKSGAVDDYFLEHS
AYPLINDAAAFFADYVEYNDTLQAYTTHNLTDPDEYANHVDNGAYTNAAISATFKWAAAV
SEHLGKPVPKVYNDIMDNVHLPRSADDPNIILEFTGMNSSIGIKQADVVMITYPLENEMI
TSESARASMEYASMKQVSFGPAMTFPIFSIVSSALSERGCSADTYLVKAIQPFLRGPFAQ
FSEQNSDDYLSNGGTHPAFPFMTAHGGFLQAILQGLLGLRFSHKTEDGKIIRFLKLDPIK
LKTLPSGVYFDGVHYMNQTLSFNLTSDGLHVMHQGPMKGMKPKKIKIELGSRNPDAGEHT
LMPGTDVHFNLYETTDSFQDSLSECGKAIFTNITECAPGDSTVLANDGDNTTHWQAISPE
KSKILIDFREVVELQNCSINWGDKPPRSVSLSAAAQDLNSDHRDLLDSIDFLSHVDFNTD
LPEKYKFNNPTGKLYKQKSIFATVASTSVEVNEPFRPEEEALIVLPSRHNTTDLTLDTPI
RTRFALLEFEGVHDKTSDDDTNSQKGAKIFELNFF

Enzyme Prediction     

EC	3.2.1.28:7

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH65	704	1063	3.2e-87	0.9946236559139785

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224471	ATH1	1.39e-80	376	1104	32	733	Trehalose and maltose hydrolase (possible phosphorylase) [Carbohydrate transport and metabolism].
369747	MIS13	2.58e-39	62	345	4	282	Mis12-Mtw1 protein family. Mis12-Mtw1 is a eukaryotic conserved kinetochore protein that is involved in chromosome segregation.
281612	Glyco_hydro_65m	1.55e-35	704	1061	1	382	Glycosyl hydrolase family 65 central catalytic domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. The central domain is the catalytic domain, which binds a phosphate ion that is proximal the the highly conserved Glu. The arrangement of the phosphate and the glutamate is thought to cause nucleophilic attack on the anomeric carbon atom. The catalytic domain also forms the majority of the dimerization interface.
397616	Glyco_hydro_65N	9.84e-21	368	643	11	240	Glycosyl hydrolase family 65, N-terminal domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. This domain is believed to be essential for catalytic activity although its precise function remains unknown.
237517	PRK13807	4.91e-17	492	926	105	546	maltose phosphorylase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QWU86795.1|GH65	0.0	321	1355	34	1068
QRG36869.1|GH65	0.0	322	1355	41	1082
QEO21230.1|GH65	0.0	325	1355	44	1082
QWW21864.1|GH65	0.0	325	1355	63	1101
QEL58660.1|GH65	0.0	325	1355	44	1082

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7FE3_A	7.13e-41	432	1059	62	640	Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_A Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101]
3WIQ_A	6.70e-24	564	1139	169	759	Crystal structure of kojibiose phosphorylase complexed with kojibiose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_A Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_B Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_C Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_D Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903]
4KTP_A	3.94e-14	618	936	221	549	Chain A, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTP_B Chain B, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10]
4KTR_A	5.17e-14	618	936	221	549	Chain A, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_B Chain B, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_C Chain C, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_D Chain D, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_E Chain E, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_F Chain F, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_G Chain G, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_H Chain H, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5AAU5|ATC1_CANAL	0.0	327	1355	78	1078	Cell wall acid trehalase ATC1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ATC1 PE=1 SV=2
sp|M1RNJ8|ATH1_CANGB	3.41e-213	335	1352	145	1208	Periplasmic/secreted acid trehalase ATH1 OS=Candida glabrata OX=5478 GN=ATH1 PE=1 SV=1
sp|P48016|ATH1_YEAST	9.01e-203	333	1232	106	1035	Periplasmic acid trehalase ATH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ATH1 PE=1 SV=1
sp|N1P212|ATH1_YEASC	9.01e-203	333	1232	106	1035	Periplasmic acid trehalase ATC1 OS=Saccharomyces cerevisiae (strain CEN.PK113-7D) OX=889517 GN=CENPK1137D_1727 PE=1 SV=1
sp|P78617|TREA_EMENI	1.17e-118	352	1351	59	1033	Acid trehalase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=treA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000064	0.000000

TMHMM  Annotations     

There is no transmembrane helices in CXQ87_001158-t45_1-p1.