CAZyme Information: CTRG_02280-t43_1-p1

Basic Information

• Species: Candida tropicalis
• Lineage: Ascomycota; Saccharomycetes; ; Debaryomycetaceae; Candida; Candida tropicalis
• CAZyme ID: CTRG_02280-t43_1-p1
• CAZy Family: GH5
• CAZyme Description: beta-glucan synthesis-associated protein SKN1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
718	GG692397|CGC3	81185.14	4.2258

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CtropicalisMYA3404	6441	294747	187	6254

• Gene Location: location=GG692397:414541-416697(+)

Full Sequence      

MARDLTYNAPKIRFVDSEGHEENFQFSNNNSTTDLNSQSSTVNNNNNNQIQNPFSDQSNT
NSSDSIQFNVSTPTNDRFPNNQQQPIINYSETNSIYSDSPNTTQRSQFQPLLNSTYRQSS
PIPVPISNESNNNLNNNNSIMTQPSGLDRYPKHVQPSSSTSQQSISFQNSNITKDINSSI
DNNSNYSSSSFTQNSSNELMGIYDNSQDFSPFGGYPASSFPLSIDEKEPDDYLHNPDPIQ
DAEYDKNRFIYDLKNMDKRSMGGLIGFIILLIGALAIFIVLPVFTFAQYGSKITTMKYEI
LTDYTYPMLSAIRQNLVDPDTPEDALTWISKDGDEWVLVFSDEFNAIGRTFYEGDDQFFT
APDFHYAATQDLEWYDPDAVTTENGTLNLRLDAFRNHNLLYRSGMVQSWNQMCFTQGKLE
FSARLPGYGNITGLWPGLWSMGNLGRPGYLASTEGVWPYTYDSCDAGITPNQSSPDGISY
LPGQRLNKCTCPGEDHPNPGVGRGAPEIDALEGEIHGIIGRVSQSLQIAPYDIWYYPDYD
YMEIHNSSVTLMNTYTGGPFQQAVSGVTMLNTTWYQFGDNANNFQKYGYEYLNDDENGYL
RWFVGDDPTFTMHAKALHPNGNIGWRQLPKEPISLIMNLGISNSWAYIDWPSLMFPSTMR
IDYVRVYQPPDAINVGCDPDDYPTYDYIQNHLNIYHNMNLTTFEQGGYTFPKHKLTGC

Enzyme Prediction     

No EC number prediction in CTRG_02280-t43_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	342	667	1.4e-159	0.9969325153374233

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	0.0	220	717	1	498	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	5.64e-166	338	668	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	2.76e-85	291	681	4	355	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	6.42e-24	340	667	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185683	Glyco_hydrolase_16	8.36e-04	531	667	90	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAA19594.1|GH16_2	0.0	1	718	1	737
AOW28590.1|GH16_2	0.0	1	718	1	737
CAX43074.1|GH16_2	0.0	1	718	1	747
CCG25571.1|GH16_2	0.0	1	718	1	697
CCE42171.1|GH16_2	0.0	80	718	91	709

PDB Hits     

CTRG_02280-t43_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P87024|SKN1_CANAX	0.0	1	718	1	737	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P87023|KRE6_CANAX	1.23e-310	142	718	154	740	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|P32486|KRE6_YEAST	4.06e-272	185	717	181	709	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P33336|SKN1_YEAST	8.43e-272	138	717	175	761	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|O13941|YEPB_SCHPO	2.71e-167	269	715	167	623	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000044	0.000001

TMHMM  Annotations     

Start	End
264	286