CAZyme Information: CPAR2_108720-T-p1

Basic Information

• Species: Candida parapsilosis
• Lineage: Ascomycota; Saccharomycetes; ; Debaryomycetaceae; Candida; Candida parapsilosis
• CAZyme ID: CPAR2_108720-T-p1
• CAZy Family: GH114
• CAZyme Description: Ortholog(s) have trehalose-phosphatase activity, role in cellular response to heat, fungal-type cell wall organization, trehalose metabolic process and alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) localization

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
882		100029.62	6.5685

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CparapsilosisCDC317	5984	578454	147	5837

• Gene Location: location=Contig005569_C_parapsilosis_CDC317:1833166-1835814(-)

Full Sequence      

MPELSQLKVSPANIHAVSPKDFANNDKVKLSGRILNVSTTLPTQIVSNYDSETNRYYWDV
DIVHGTSALFSSQSFLQQHTDWETHVIAWTGELKNKVKNTVNVTADNLEDDPLYLDDEDK
DEIEKKLQKAIGSKNIHPIWLLRRDQERWRKYAESVIWPVFHYFSGTPSDGKDEIQAWHD
YVKFNEAYANKIKQIYKPGDVIWIHDYYLLLLPQLLRMEFPDAYIGHFLHVPFPSSEYFR
CLSKRSQLLDGLLGADKIGFQSESFQRHFVSCCARVLGCEVTKNQIFAYGTTISVETLPI
GIDTLKIESDAFSKSSGIDQKVEAIRNLYRDKKIIVGRDRLDRVRGVVQKLEAFSMFLEM
YPEWREKVVLIQVSTPGYSHSANVEQKATELINQINSRYGTLNHTPVLHYQMRVPKEEYL
ALLRVADLCLITSVRDGMNTTAFEFVICQKYNNSPLILSEFTGTASVLNSAILVNPWDSV
GVSKTINDALSFSDHEKEVLESTLYKKVASNTIQNWTSTFIRDIIDHSIITHSSHYTPAL
NRPLLLRNYTHSKRRLFLFDYDGTLTPIVKDPAAAIPSDKLNRVLDILASDPKNQIWIIS
GRDQAFLEKWMGRKHVGLSAEHGCFIKDLGSKEWVNLAEAYDMSWQPKVEDVFKKYTKQT
PGSNIEIKKVALTWHYRGADPELGAYQAQKCLDELNDTIATEYDVEVMSGKANIEVRPKF
LNKGEIVKRLVTHPHGTKQSLEEELNKEVSHEELPDFVLCLGDDLTDEDMFKSLHKIENQ
WKLDNVTKNEFGTYGVFPVAVGPASKKTVATAHLTEPAQVLETLGLLAGTVSLFESAGSV
DLDDRGHVANSISSVKSKDAIREASLRKKRSIELNGTTAAAK

Enzyme Prediction     

EC	3.1.3.12:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT20	22	524	1.6e-158	0.9894736842105263

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
184712	PRK14501	0.0	79	828	38	724	putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional
340820	GT20_TPS	0.0	33	524	1	462	trehalose-6-phosphate synthase. Trehalose-6-Phosphate Synthase (TPS, EC 2.4.1.15) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain.
395781	Glyco_transf_20	4.74e-171	116	524	58	468	Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex,.
223457	OtsA	7.07e-155	119	524	70	477	Trehalose-6-phosphate synthase [Carbohydrate transport and metabolism].
215555	PLN03063	4.00e-130	33	771	12	717	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCE40834.1|GT20	0.0	1	882	1	882
CCG22685.1|GT20	0.0	1	880	1	880
AOW26019.1|GT20	0.0	6	873	5	875
CAC17748.1|GT20|3.1.3.12	0.0	6	873	5	875
CAX44578.1|GT20	0.0	6	873	5	875

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5DXF_A	3.22e-298	6	534	5	533	Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [Candida albicans SC5314],5DXF_B Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [Candida albicans SC5314]
5DXI_A	1.30e-165	533	832	1	302	Structure of C. albicans Trehalose-6-phosphate phosphatase C-terminal domain [Candida albicans SC5314],5DXI_B Structure of C. albicans Trehalose-6-phosphate phosphatase C-terminal domain [Candida albicans SC5314]
5DXN_A	5.40e-96	537	827	3	269	Structure of Aspergillus fumigatus trehalose-6-phosphate phosphatase crystal form 2 [Aspergillus fumigatus Af293],5DXO_A Structure of Aspergillus fumigatus trehalose-6-phosphate phosphatase crystal form 3 [Aspergillus fumigatus Af293],5DXO_B Structure of Aspergillus fumigatus trehalose-6-phosphate phosphatase crystal form 3 [Aspergillus fumigatus Af293]
5DXL_A	6.62e-92	537	827	3	269	Structure of Aspergillus fumigatus trehalose-6-phosphate phosphatase crystal form 1 [Aspergillus fumigatus Af293]
5HUV_A	1.01e-86	152	524	92	467	Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314],5HUV_B Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P31688|TPS2_YEAST	0.0	23	858	5	877	Trehalose-phosphatase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPS2 PE=1 SV=3
sp|Q9P4D3|TPS2_ZYGRO	4.61e-314	33	871	10	897	Trehalose-phosphatase OS=Zygosaccharomyces rouxii OX=4956 GN=TPS2 PE=1 SV=1
sp|P78875|TPP1_SCHPO	3.43e-205	30	828	35	813	Trehalose-phosphatase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tpp1 PE=1 SV=2
sp|O14145|TPS2_SCHPO	6.41e-158	152	824	172	846	Trehalose-phosphatase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tps2 PE=3 SV=1
sp|Q54NU9|TPSB_DICDI	3.68e-149	124	777	111	753	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] B OS=Dictyostelium discoideum OX=44689 GN=tpsB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999225	0.000809

TMHMM  Annotations     

There is no transmembrane helices in CPAR2_108720-T-p1.