CAZyme Information: CPAG_06821-t26_1-p1

Basic Information

• Species: Coccidioides posadasii
• Lineage: Ascomycota; Eurotiomycetes; ; Onygenaceae; Coccidioides; Coccidioides posadasii
• CAZyme ID: CPAG_06821-t26_1-p1
• CAZy Family: GT2
• CAZyme Description: endo-1,3(4)-beta-glucanase 1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
888		96861.06	8.1895

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CposadasiiRMSCC3488	10016	454284	119	9897

• Gene Location: location=DS268112:2028198-2031194(-)

Full Sequence      

MVQLTKMWLSQAVLIFLGILLSAESRPLSLDPDARVGDVPLTALFRVPPIANRAVSLLET
STETSAPSALQRSPVGDISPTPTTQNWARPAQAQLGFSSPRRQPLKPENPTPRVPSRIQA
AISYFNTSSDRTLTTTLGPTGPTRPTSPTGTGIVSSTSPTSQPSMASQNVFLPVSKDQIP
ANIPKRDGHPVRKDHITDAPGPISTNKFYANFFLGNQSDYSFIQPYSVGWSKGANPTGSF
GLAISHSDANQTVFGERSDVIPGNPVRFYANPVGIQSIILSAEELKNSTTLSLSNPQAFS
ANVILRPQKDSDASASFPLVQGMGFVTGLYDNLQPVIQSQVSFKQVDAAGSPKDGIFKYK
ASLQDGASWLLYVTPENGADPKLVLVSNNTIRGSKGFNGFIQVAKDPGNGDSIYDGSAGV
YPTAANIAGSVTGNQGTYQFSWTKAGKNANNTPLLMFALPHHVEAFDDSSKRRKTDLKLQ
TTTKGQATACVGDSWTMIEPNLPVDIGFEPWKPGMSGKPSLSAGAKEQIKAVALSELSQD
MESQTNLDSMYFSGKALGKFAGAVYTAQELLQDAGLASRALESLKKSFARFVENKQKHPL
VYDTVWKGAVSSGTYQTGDIYLDFGNTLYNDHHFHYGYFILTAAIIGHLDRSWLDTNKVW
VNMLVRDASNSVSDELFPFSRGFDWFNGHSWAKGLFASIDGKDQESTSEDAMFAYALKMW
GKTSGDASMEARGNLMLGILYRSLDSYFLMRRSNVNQPKEFIDNKVTGILFENKCDHATY
FGANLEYIQGIHMLPLLPASAYTRRSEFVREEWNAMFAPNATKPADTVEGGWRGVLYANL
AIIDAEASWKFFTQPNFNMGWIDGGASRTWYLAYAALLSRTTSFSRCK

Enzyme Prediction     

EC	3.2.1.39:20

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	193	874	7.5e-232	0.9839228295819936

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
407548	Glyco_hydro81C	0.0	520	872	1	349	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
227785	Acf2	0.0	189	879	75	756	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
397619	Glyco_hydro_81	2.29e-163	189	513	1	321	Glycosyl hydrolase family 81. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein ENGL1, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABH10633.1|GH81	0.0	165	878	1	714
BCS18746.1|GH81	2.00e-316	166	876	169	880
AAF13033.2|GH81|3.2.1.39	9.99e-316	152	876	2	721
CAP93568.1|GH81	2.28e-314	164	876	176	889
CBF89420.1|GH81|3.2.1.39	2.34e-312	165	876	187	899

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5XBZ_A	6.34e-105	161	876	24	741	Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XBZ_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XC2_A Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_C Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_D Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei]
4K3A_A	1.86e-104	169	876	7	716	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K3A_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
4K35_A	2.77e-101	169	876	7	716	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K35_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
6FOP_A	1.16e-23	534	854	324	636	Chain A, Glycoside hydrolase family 81 [Acetivibrio thermocellus ATCC 27405]
5V1W_A	1.68e-20	627	802	436	611	Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4AZ24|ENG1_ARTBC	0.0	96	876	130	900	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
sp|Q09850|ENG2_SCHPO	3.04e-173	169	876	2	700	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1
sp|Q12168|ENG2_YEAST	4.10e-165	155	879	60	779	Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1
sp|Q5AIR7|ENG1_CANAL	3.71e-161	202	876	460	1139	Endo-1,3(4)-beta-glucanase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ENG1 PE=1 SV=1
sp|Q9UT45|ENG1_SCHPO	2.05e-158	169	876	46	735	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000349	0.999612	CS pos: 25-26. Pr: 0.9687

TMHMM  Annotations     

There is no transmembrane helices in CPAG_06821-t26_1-p1.