CAZyme Information: CPAG_05732-t26_1-p1

Basic Information

• Species: Coccidioides posadasii
• Lineage: Ascomycota; Eurotiomycetes; ; Onygenaceae; Coccidioides; Coccidioides posadasii
• CAZyme ID: CPAG_05732-t26_1-p1
• CAZy Family: GH63
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
499		55267.56	8.2482

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CposadasiiRMSCC3488	10016	454284	119	9897

• Gene Location: location=DS268111:3020211-3022279(-)

Full Sequence      

MNDTSQYFTHIIYSNFTQYSPGKKLASNPEFRRNWAIQRWNMPSINWATPLPVLNEQENV
YLENGHMVLRQVGYPKEGILAGRNVSVASIAGQSGDLLHGSFRTEVKIEGAEGGSVGAFF
WYHDDQNEIDIEVLTREIKSDRLLVHYTTHPAIDEDGDVIRNATAIVPVEGNDPANWFQR
HRFDWSKEELRFYHNGSLMHANNIRIPDVGGRALLNLWADGGIWSGAPSTTNVYMRVKYV
IVYHNTTASDSGQDVEFNDRCARAGGPSNETVCLDVFVEDGAVDPSSIGTALAPLPFWYN
PSPPYALKQPAHNRYTRGLVGRREVRVGNRRVSSEAGSIPARPISAQSGLLSFVLGNNLV
VPPRAAAGGGGTSPVLEPLCILRASGRIGGRGDHERATLESIRVGVRPALFARRRFLAVF
PLCRRVTGSHLADLTIWISDLPLETLCPSCLRAVLPRYLPPDADGGYRKLHALEARMARR
EVPLPVPHAVLYVFRNSGF

Enzyme Prediction     

No EC number prediction in CPAG_05732-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	83	243	1.5e-33	0.859375

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185683	Glyco_hydrolase_16	2.83e-40	27	243	5	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
185684	GH16_lichenase	6.69e-10	99	240	68	208	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
185693	GH16_laminarinase_like	2.85e-06	58	243	38	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185687	GH16_beta_agarase	2.78e-05	38	200	37	204	Beta-agarase, member of glycosyl hydrolase family 16. Beta-agarase is a glycosyl hydrolase family 16 (GH16) member that hydrolyzes the internal beta-1,4-linkage of agarose, a hydrophilic polysaccharide found in the cell wall of Rhodophyceaea, marine red algae. Agarose is a linear chain of galactose units linked by alternating L-alpha-1,3- and D-beta-1,4-linkages that are additionally modified by a 3,6-anhydro-bridge. Agarose forms thermo-reversible gels that are widely used in the food industry or as a laboratory medium. While beta-agarases are also found in two other families derived from the sequence-based classification of glycosyl hydrolases (GH50, and GH86) the GH16 members are most abundant. This domain adopts a curved beta-sandwich conformation, with a tunnel-shaped active site cavity, referred to as a jellyroll fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS61966.1|GH16	7.19e-78	101	298	1	183
EAA58218.1|GH16_22	9.54e-27	31	247	81	292
BCS21543.1|GH16_22	1.16e-26	27	247	74	289
QRV85770.1|GH16	5.01e-24	30	273	40	277
QRV99836.1|GH16	5.01e-24	30	273	40	277

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1GBG_A	1.28e-08	105	240	76	209	Bacillus Licheniformis Beta-Glucanase [Bacillus licheniformis]
3O5S_A	2.33e-08	105	240	100	233	Crystal Structure of the endo-beta-1,3-1,4 glucanase from Bacillus subtilis (strain 168) [Bacillus subtilis]
1CPM_A	6.27e-07	105	247	18	158	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1CPN_A	1.06e-06	105	239	18	153	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1MAC_A	1.11e-06	105	239	74	209	Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans],1MAC_B Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P04957|GUB_BACSU	1.25e-07	105	240	104	237	Beta-glucanase OS=Bacillus subtilis (strain 168) OX=224308 GN=bglS PE=1 SV=2
sp|P27051|GUB_BACLI	1.70e-07	105	243	105	240	Beta-glucanase OS=Bacillus licheniformis OX=1402 GN=bg1 PE=1 SV=1
sp|P07980|GUB_BACAM	1.29e-06	105	232	101	225	Beta-glucanase OS=Bacillus amyloliquefaciens OX=1390 GN=bglA PE=3 SV=1
sp|P23904|GUB_PAEMA	7.43e-06	105	239	99	234	Beta-glucanase OS=Paenibacillus macerans OX=44252 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000053	0.000009

TMHMM  Annotations     

There is no transmembrane helices in CPAG_05732-t26_1-p1.