CAZyme Information: CPAG_00945-t26_1-p1

Basic Information

• Species: Coccidioides posadasii
• Lineage: Ascomycota; Eurotiomycetes; ; Onygenaceae; Coccidioides; Coccidioides posadasii
• CAZyme ID: CPAG_00945-t26_1-p1
• CAZy Family: AA6
• CAZyme Description: UDP-glucose:glycoprotein glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1488		167985.44	5.6908

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CposadasiiRMSCC3488	10016	454284	119	9897

• Gene Location: location=DS268109:3086992-3091726(-)

Full Sequence      

MPFKAASLPWQLGLFLGALLLPPAVQASRSVNVALSASFSSPPYLLELLETAAEENSTSY
FPLLDRIADGAFAESRTDKDLYDQFLRVLRDEGHIRDPDALSSFKLAMSLRSTAPRIQAH
YQYYNTSVEQSLMVVQDAVCQVWAHYGGEQHCSPSLKHAQQSVSGDQDDHVLPFDRVLGR
GDQPLVLYADISSPLFGDFHKELSKRAVEGEFSYRVRYRPSSLGHANRLFVSGYGVELAL
KRTDYIVIDDRDAEQSSSSSSSSSEKAKATHGTAKDLKEESPADLKPLSASEVSTLGINA
ASFIMNSEDPFETLLKLSQDFPRHSSIIANTNATIEFLVELANNQAHMLPAGYNVMWING
VQLDPRRTNAFSLLNHLRYERKVINNFRELDLSAGDVVRLLAHPVVARSQAIEEAPRYDY
RDETEGGGVIIWLNDLEKDRRYSNWSRGLAALLQRTYPGQFPQARRDVNNVIVLLNLADR
TDLRMLVSQLQMFITRKIPIRFGMVPTLPDEASMQQIRVASYLHQTYGLKTLLTYFENAL
EGAKSQIVWPSKDSFNAAVQDREHHADRPKLTFEEILSSDHFEPIITKTKAYLKRLSSDG
PNPPMFVNGAIIPRDEHWMQPLVTRLAQDLEEIQQAIYGGLYDDDSWLPIHFLDGAVLTR
NPLIIPEDPGAVQIRDLHAAFKSRRSAFDALPRIRASSDSNLENWSSLILIADFDSEGGI
KQLGSVLEFREKNPGIEVLLLHDSHLDFSGRVSAELFNLMKESRDVDVSALKSILEVGSE
RLLTQEPDVERRRNYFSSFSPLARELGSNQGGVDIVFNGRLIGPIPSSSLFGVQELEQLL
AYERQRRLEPLFGAVKSLELKDVVDGPFKLARLTSLVARSTKLDIPEDIYDSGPALRTNS
YEKWSTEHSGFMLSHADDPVIQVVAVIDPASEAAQRYIPILKVLSKLSGVSVKVILSPIQ
PLKELPIKRFYRQVFESEPSFNDDGSLRRPEASFTSIPEDALLTLGMDVAPSWLVAPKES
VYDLDNIKLSSLREGANVDAVYELEHILIEGHSRDVTHGSPPRGVQLLLGTEKTPHFADT
IIMANLGYFQFKAQPGCWRITLKPGPSERIYQLDSVGGMGYVPKPGDETNEVALLSFQGT
TLFPRLSRKPGHEEDDVLEAGSKAGSVKNYFSKGLNFASDVISSITGSQKEKHADINIFS
VASGHLYERMLNIMMVSVMRHTKHSVKFWFIEQFLSPSFKSFLPHLAKEYGFSYEMVTYK
WPHWLRSQREKQREIWGYKILFLDVLFPLSLDKVIFVDADQIVRTDMYDLITMDLDGAPY
GFTPMCDSRTEMEGFRFWKQGYWKKFLKGLPYHISALYVVDLNRFRELAAGDRLRGQYQS
LSADPNSLANLDQDLPNHMQHAIPIKSLSQDWLWCETWCSDEALKTARTIDLCNNPMTKE
PKLERARRQVPEWTEYDDEIAELGRRVAREQGQAGDEKNEKMRERDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1196	1443	7.6e-135	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1196	1463	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	0.0	1196	1443	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408201	Thioredoxin_14	2.83e-94	418	664	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
408199	Thioredoxin_12	1.61e-63	41	222	1	180	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	6.42e-62	1050	1159	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS69256.1|GT24	0.0	1	1471	1	1470
QSS63494.1|GT24	0.0	7	1488	18	1518
QSS51253.1|GT24	0.0	1	1471	1	1470
QRD91624.1|GT24	0.0	10	1474	11	1466
QMW47293.1|GT24	0.0	10	1474	11	1466

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	25	1470	5	1454	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	25	1470	5	1454	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	27	1165	2	1130	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	25	1470	5	1454	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	25	1470	5	1220	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	2.14e-291	27	1463	18	1424	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	3.04e-236	28	1470	44	1530	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q9JLA3|UGGG1_RAT	6.05e-234	28	1463	44	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	8.47e-234	28	1463	44	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q09332|UGGG_DROME	3.38e-231	18	1467	15	1511	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000210	0.999777	CS pos: 27-28. Pr: 0.9762

TMHMM  Annotations     

There is no transmembrane helices in CPAG_00945-t26_1-p1.