CAZyme Information: CNE01990-t26_1-p1

Basic Information

• Species: Cryptococcus neoformans
• Lineage: Arthropoda; Insecta; ; Eriococcidae; Cryptococcus; Cryptococcus neoformans
• CAZyme ID: CNE01990-t26_1-p1
• CAZy Family: GH152
• CAZyme Description: endochitinase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
582	AE017345.1|CGC2	62288.47	4.0268

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CneoformansJEC21	7004	214684	372	6632

• Gene Location: location=AE017345.1:535494-537723(-)

Full Sequence      

MYCTLATLSLLALAEAAGPHNRRLDDHRRHYARIALSGESETPPLPHNLHVEVPTKRDGC
THGGWNCVGLQLQRCNWGDWIVIQNCTGENIVCSTQEDAVGCVWTWFVDDSTTSQGAQGT
PSATTSLVFDASSAVSASYVSATPSQGLDDGEPTGTASSYYATATTSTTASDKNDQACDD
DDDDDDEVCEDEDASSTASGTFNPATITSSATFSTSDAQGTLTSQIGDGLLAGHKSESAS
SSDDWRETAGSDHKSRTKQWGSWGTSSASDGSAPTTNASVSYPAATASSTTTAASSSHSA
PTNLGGWSAPHYVIYADRWLSAMPDVSELSSFNRFILAFWESNGGAVDDVQAWSSWDEGY
RRQIIEEYHDAGIAIMIAAFGSTDQPTTSGADAKQVAQKLASFVMEYNLDGVDIDYEDMS
AMNSAQAVSWIVELQMELRNLLPSPYIISHAPVAPWFTSANDYSDGSYVSIHQQVGDSID
FYSVQFYNQGPDQYVSCETLITDSGSEWPSTSVFEINSHAGVPLDKIVIGKPLEPGSASN
GYMSASDLHQCVSEAKERGWNAGVMFWEWSEEAPSVMATVRG

Enzyme Prediction     

No EC number prediction in CNE01990-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	331	562	4.4e-19	0.6722972972972973

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
119349	GH18_chitinase-like	8.10e-33	312	572	1	210	The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model.
119350	GH18_chitinase_D-like	1.12e-15	388	567	90	293	GH18 domain of Chitinase D (ChiD). ChiD, a chitinase found in Bacillus circulans, hydrolyzes the 1,4-beta-linkages of N-acetylglucosamine in chitin and chitodextrins. The domain architecture of ChiD includes a catalytic glycosyl hydrolase family 18 (GH18) domain, a chitin-binding domain, and a fibronectin type III domain. The chitin-binding and fibronectin type III domains are located either N-terminal or C-terminal to the catalytic domain. This family includes exochitinase Chi36 from Bacillus cereus.
119363	GH18_CTS3_chitinase	1.65e-15	378	569	82	253	GH18 domain of CTS3 (chitinase 3), an uncharacterized protein from the human fungal pathogen Coccidioides posadasii. CTS3 has a chitinase-like glycosyl hydrolase family 18 (GH18) domain; and has homologs in bacteria as well as fungi.
119356	GH18_hevamine_XipI_class_III	7.10e-10	371	568	71	264	This conserved domain family includes xylanase inhibitor Xip-I, and the class III plant chitinases such as hevamine, concanavalin B, and PPL2, all of which have a glycosyl hydrolase family 18 (GH18) domain. Hevamine is a class III endochitinase that hydrolyzes the linear polysaccharide chains of chitin and peptidoglycan and is important for defense against pathogenic bacteria and fungi. PPL2 (Parkia platycephala lectin 2) is a class III chitinase from Parkia platycephala seeds that hydrolyzes beta(1-4) glycosidic bonds linking 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin.
395573	Glyco_hydro_18	1.13e-09	336	532	27	221	Glycosyl hydrolases family 18.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAW43533.1|GH18	0.0	1	582	1	582
AFR95478.1|GH18	0.0	1	582	1	634
AUB25287.1|GH18	0.0	1	582	1	634
UOH82414.1|GH18	7.85e-297	1	575	1	640
ADV22450.1|GH18	1.71e-276	1	582	1	550

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6K7Z_A	3.17e-21	392	572	92	265	Crystal structure of a GH18 chitinase from Pseudoalteromonas aurantia [Pseudoalteromonas aurantia],6K7Z_B Crystal structure of a GH18 chitinase from Pseudoalteromonas aurantia [Pseudoalteromonas aurantia],6K7Z_C Crystal structure of a GH18 chitinase from Pseudoalteromonas aurantia [Pseudoalteromonas aurantia],6K7Z_D Crystal structure of a GH18 chitinase from Pseudoalteromonas aurantia [Pseudoalteromonas aurantia]
4RL3_A	9.92e-07	363	496	64	198	Crystal Structure of the Catalytic Domain of a family GH18 Chitinase from fern, Peteris ryukyuensis [Pteris ryukyuensis],4RL3_B Crystal Structure of the Catalytic Domain of a family GH18 Chitinase from fern, Peteris ryukyuensis [Pteris ryukyuensis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A5FB63|CHIA_FLAJ1	7.47e-07	365	547	1211	1421	Chitinase ChiA OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) OX=376686 GN=chiA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000298	0.999669	CS pos: 16-17. Pr: 0.9729

TMHMM  Annotations     

There is no transmembrane helices in CNE01990-t26_1-p1.