CAZyme Information: CNAG_05188-t26_2-p1

Basic Information

• Species: Cryptococcus neoformans
• Lineage: Arthropoda; Insecta; ; Eriococcidae; Cryptococcus; Cryptococcus neoformans
• CAZyme ID: CNAG_05188-t26_2-p1
• CAZy Family: GH71
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
316		35960.57	4.6346

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CneoformansH99	8338	235443	1363	6975

• Gene Location: location=CP003823.1:672675-673750(-)

Full Sequence      

MTPPSAKFTRASNTANVVTFVALSSFFFSVLYYALSPSPLLPFDTLSSYNAQSNKSNDMP
REAYVTFLSNEDPYYFQSARLLVFALQHDPLTADPSRPVIVLTTPSVPASYSRKLEAEGA
IVIEKPLITSLPTVQTNPRWKDVYTKLWIFNMTSYDRLVYYDADHLVLRPVDSIWEAENS
WPESALAALGSGDGGYVEDSDYFLAGFFIAIPKDEIMEGLLAEKGYDPVFPEQNLMNKYF
SRDGPRPWAPLDPIHERISPEYEHVEQGAHTIHEKCWQGWVERRLAELFYERLGRMEGYW
LAKEFNGTIPTPDPRG

Enzyme Prediction     

No EC number prediction in CNAG_05188-t26_2-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT8	50	253	4e-21	0.8249027237354085

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
133018	GT8_Glycogenin	1.47e-61	62	305	1	240	Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases.
227884	Gnt1	2.12e-09	63	253	72	313	Alpha-N-acetylglucosamine transferase [Cell wall/membrane/envelope biogenesis].
215090	PLN00176	4.03e-08	42	247	3	229	galactinol synthase
133064	GT8_GNT1	1.17e-07	63	172	2	109	GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases.
132996	Glyco_transf_8	2.43e-04	135	240	75	197	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUB24114.1|GT8	2.08e-238	1	316	1	316
AFR94453.2|GT8	2.08e-238	1	316	1	316
AAW46473.2|GT8	2.37e-219	1	316	1	316
UOH84644.1|GT8	1.41e-216	1	316	1	299
KGB78671.1|GT8	1.41e-211	1	316	1	316

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3V90_A	9.52e-08	55	252	17	198	Structure of T82M glycogenin mutant truncated at residue 270 [Oryctolagus cuniculus],3V91_A Structure of T82M glycogenin mutant truncated at residue 270 complexed with UDP-glucose [Oryctolagus cuniculus]
1ZCT_A	1.27e-07	55	252	17	198	structure of glycogenin truncated at residue 270 in a complex with UDP [Oryctolagus cuniculus],1ZCT_B structure of glycogenin truncated at residue 270 in a complex with UDP [Oryctolagus cuniculus]
3USQ_A	1.28e-07	55	252	17	198	Structure of D159S/Y194F glycogenin mutant truncated at residue 270 [Oryctolagus cuniculus]
3V8Y_A	1.28e-07	55	252	17	198	Structure of apo-glycogenin truncated at residue 270 [Oryctolagus cuniculus],3V8Z_A Structure of apo-glycogenin truncated at residue 270 complexed with UDP [Oryctolagus cuniculus]
3USR_A	1.28e-07	55	252	17	198	Structure of Y194F glycogenin mutant truncated at residue 270 [Oryctolagus cuniculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09680|YA0C_SCHPO	2.49e-10	66	277	76	340	Uncharacterized protein C5H10.12c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC5H10.12c PE=4 SV=1
sp|Q4PSY4|GOLS7_ARATH	3.15e-08	54	258	11	235	Galactinol synthase 7 OS=Arabidopsis thaliana OX=3702 GN=GOLS7 PE=2 SV=1
sp|Q947G8|GOLS1_SOLLC	7.16e-08	63	240	21	210	Galactinol synthase 1 OS=Solanum lycopersicum OX=4081 GN=GOLS1 PE=2 SV=1
sp|O43061|MU136_SCHPO	1.15e-07	35	278	51	337	Meiotically up-regulated gene 136 protein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mug136 PE=1 SV=1
sp|O22693|GOLS4_ARATH	2.45e-07	60	242	21	226	Galactinol synthase 4 OS=Arabidopsis thaliana OX=3702 GN=GOLS4 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999996	0.000001

TMHMM  Annotations     

Start	End
13	35