CAZyme Information: CNA06670-t26_1-p1

Basic Information

• Species: Cryptococcus neoformans
• Lineage: Arthropoda; Insecta; ; Eriococcidae; Cryptococcus; Cryptococcus neoformans
• CAZyme ID: CNA06670-t26_1-p1
• CAZy Family: AA5
• CAZyme Description: beta-1,4-mannosyltransferase, putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
506		57005.96	7.0852

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CneoformansJEC21	7004	214684	372	6632

• Gene Location: location=AE017341.1:1820674-1822345(+)

Full Sequence      

MEGRYNPFEDPDTIPVYQIFLLFITFVGPPTFLFVLFVRRTATRPHLHHTATVLVLGDIG
RSPRMMYHSESLARHNWRTFMVGYAETPPTSALLENPMVHLLGLKEPPKMVGLLPWILRA
PIRIIYQVFSVIHTCIWRVPCNTEILLVQNPPSIPTLALAQFICLATKTKLIIDWHNTGY
SILGLRVGEGSRLVRIAKWFESTFGQTAYAHLFVTKALQEFLIREWDLKGRTSVLHDRPP
THFHRTVPMIQHELFSRLLPELKPSLPPSHLDTNDPTHTAFTEISSEGLAVLKHTRPALI
ISSTSWTADEDFSLLITALDLYQSAVDSGSALPKLVVLITGKGALRAPFENIVKSREISK
WKDITVRCVFVPAQEYPPLLGCADLGVSLHTSSSGKDLPMKVVDMFGCGVPVLAKDFQCI
SELVKDGENGKVFGTGEELGEQMIDILSSFPSSEKLDDLKGYFDRMNTPRRRATLPTADV
GEDEWSNWDDNWDNVVYNGILNKIRR

Enzyme Prediction     

EC	2.4.1.142:10	2.4.1.-:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT33	49	497	1.2e-158	0.9905882352941177

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340843	GT33_ALG1-like	0.0	46	500	1	411	chitobiosyldiphosphodolichol beta-mannosyltransferase and similar proteins. This family is most closely related to the GT33 family of glycosyltransferases. The yeast gene ALG1 has been shown to function as a mannosyltransferase that catalyzes the formation of dolichol pyrophosphate (Dol-PP)-GlcNAc2Man from GDP-Man and Dol-PP-Glc-NAc2, and participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. In humans ALG1 has been associated with the congenital disorders of glycosylation (CDG) designated as subtype CDG-Ik.
215155	PLN02275	2.58e-130	49	447	5	371	transferase, transferring glycosyl groups
223515	RfaB	3.30e-09	90	456	25	349	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
340831	GT4_PimA-like	5.31e-08	60	448	15	332	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.
395425	Glycos_transf_1	6.63e-07	321	456	20	151	Glycosyl transferases group 1. Mutations in this domain of PIGA lead to disease (Paroxysmal Nocturnal haemoglobinuria). Members of this family transfer activated sugars to a variety of substrates, including glycogen, Fructose-6-phosphate and lipopolysaccharides. Members of this family transfer UDP, ADP, GDP or CMP linked sugars. The eukaryotic glycogen synthases may be distant members of this family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAW41187.1|GT33	0.0	1	506	1	506
AUB22294.1|GT33	0.0	1	506	1	506
AFR92818.1|GT33	0.0	1	506	1	506
ADV19846.1|GT33	0.0	1	506	1	506
KGB75402.1|GT33	0.0	1	506	1	506

PDB Hits     

CNA06670-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5R7A2|ALG1_PONAB	1.64e-101	39	496	27	457	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Pongo abelii OX=9601 GN=ALG1 PE=2 SV=1
sp|Q9BT22|ALG1_HUMAN	6.33e-98	54	496	38	457	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Homo sapiens OX=9606 GN=ALG1 PE=1 SV=2
sp|Q921Q3|ALG1_MOUSE	1.07e-97	39	496	27	457	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Mus musculus OX=10090 GN=Alg1 PE=1 SV=3
sp|Q8L7M0|TUN_ARATH	2.90e-89	51	453	7	423	UDP-glycosyltransferase TURAN OS=Arabidopsis thaliana OX=3702 GN=TUN PE=2 SV=1
sp|P90522|ALG1_DICDI	3.27e-85	53	488	6	455	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Dictyostelium discoideum OX=44689 GN=alg1 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000079	0.000000

TMHMM  Annotations     

Start	End
15	37