CAZyme Information: CLCR_03250-t43_1-p1

Basic Information

• Species: Cladophialophora carrionii
• Lineage: Ascomycota; Eurotiomycetes; ; Herpotrichiellaceae; Cladophialophora; Cladophialophora carrionii
• CAZyme ID: CLCR_03250-t43_1-p1
• CAZy Family: GH16
• CAZyme Description: UDP-glucose:glycoprotein glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1437	LGRB01000013|CGC3	161120.49	4.9602

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CcarrioniiKSF	11240	N/A	59	11181

• Gene Location: location=LGRB01000013:1395368-1399912(-)

Full Sequence      

MRTSNLPWRCALLPLTEQELYTKFLDVLQDEGHISNPESLSSFQFALVLHAAAPRVEAHF
QYYNTSVQPHLMAAQDAVCPVWIYLDGQQFCSPALDRAQQAVIMPDADDDLPFDRVLGTS
SDGPASILYADITHPLFGQFHGVVSQTARQGKSTYRVRYRPSHLETKKPIYVSGYGVELV
LKRTDYIVIDDRDAQKPEDAASSQLSLEDAKDDNLKDLKPLTTSEVVNLGLNAANFIAAS
SDPLETLLQVTSDFPRYSSLLASANASKEFVQEHRGNRERFLPSGYNVFWINGVQIEPRQ
INAYALLDHLRRERRIIGELKDLGLTSSEAISLLSSEVIAEAQTNELPQRFDWRDDSEAN
EVLMWLNDLEQDKRYNAWSKSLRTLFQRTYPGQLPQIARDIHNLVVPIDMGNIKDIELVA
TSLQSLVRRSVPLRIALIPTGEGETANTYAKIAYHILDTYGLTALMNFFSDVVTSKKLVS
NPEKSFNNAIKGRAVRQGKHAQPYQAVLDDVGLSLRLQARDDYLKRLALDGPDPPFLING
VVLPRMDNWFELMSARLYSDLQMIQQAVMQEAIFEESWIPEFFLFQAVKSRNELVFPDDP
KDVNIVDVGLLSSQFADVVARLPRILGSEATLLSDRAHLLVIADIDSERGRGLVSEALLF
YQRYPEIEMLVLHNPSGEAQNSNYANALHALVQNGARDLTVAQLQQLLGDDHITSADGSS
ESSADFWSSQAALARGLGLSEGQNGLWLNGRLLGPVDQPLTSADLESLLEVERRERIAPV
TTAITGLALEDRFSDALDLAKVSSLVARSLKSDLPEGLRESAPLIRMDRFKIWNGTHTSI
NLSNSADPTIQIVAVVDPASEIVQQWVPILQTLSHLGGVGVRIFLNPKDRLTELPVKRFF
RQVISAEPSFNADGSLAIPKASFDGIPRDTLFNLGMIVPPSWLVAPKQSIHDLDNIKLSS
LAEGDNIDATYELEHILIEGHARDVTNGPPPRGVQLLLGTEQEPHFTDTIVMANLGYFQF
SANLGYWQISLKPGRSSKIFNIESVGPYGYSAKPGDETTSVALLSFQGLTLFPRLSRKPG
MEEEDVLEASGFGGAMDYFSKGASFASSALSSLGFKKASTNADINIFSVASGHLYERMLN
IMMVSVMKHTKHSVKFWFIEQFLSPSFKTTLPSLAEHYGFEYEMVTYKWPYWLRGQKEKQ
REIWGYKILFLDVLFPLDLDKVIFVDADQIVRTDMMELNKVDLKGAPYGFTPMCDSRTEM
EGFRFWKQGYWQTYLRGKPYHISALYVVDLKRFRQLAAGDRLRQQYQALSADPASLSNLD
QDLPNHMQHNLPIHSLPQEWLWCETWCSDDSLAKAKTIDLCNNPQTKEPKLDRARRQVPE
WTEYDEEIAAVIRAAKEKQGNDGTRAVVAGHEDLNEAATDEGSQEKLLSDERIRDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1124	1371	2.8e-134	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
133054	GT8_HUGT1_C_like	0.0	1124	1371	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408203	Glyco_transf_24	0.0	1124	1391	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
408201	Thioredoxin_14	2.37e-79	351	595	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	1.03e-62	979	1088	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.
132996	Glyco_transf_8	6.16e-59	1124	1371	1	246	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAK39488.1|GT24	0.0	15	1399	77	1464
CAS97678.1|GT24	0.0	15	1399	77	1464
BCR94558.1|GT24	0.0	15	1398	77	1463
BCS07152.1|GT24	0.0	15	1398	77	1463
CAP81259.1|GT24	0.0	16	1410	78	1475

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	16	1399	57	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	16	1399	57	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	15	1088	51	1124	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	16	1399	57	1455	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	16	1399	57	1221	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	2.44e-273	16	1395	68	1428	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	3.00e-227	16	1401	78	1517	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	1.60e-223	21	1399	96	1532	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q6P5E4|UGGG1_MOUSE	5.87e-222	18	1388	73	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9JLA3|UGGG1_RAT	1.77e-219	63	1388	127	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000057	0.000000

TMHMM  Annotations     

There is no transmembrane helices in CLCR_03250-t43_1-p1.