CAZyme Information: CKF44_05142-t42_1-p1

Basic Information

• Species: Cryptococcus neoformans
• Lineage: Arthropoda; Insecta; ; Eriococcidae; Cryptococcus; Cryptococcus neoformans
• CAZyme ID: CKF44_05142-t42_1-p1
• CAZy Family: GH5
• CAZyme Description: dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
447		50712.83	10.2464

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CneoformansKN99	6967	N/A	0	6967

• Gene Location: location=CP022324.1:541004-542639(-)

Full Sequence      

MSHQSIYGPALRQRKGLIRHAVDLVRALLFDRRYFWHTAFLLFLGEVALGLLVIWKIPYT
KIDWPAYMQQVDMFLAGERDYSKIEGETGPLVYPALHLYIYTAFHRLLPSIENVRPAQFV
FLGFYLATYLAVSTIYYLAGRPSNGGHHFPQVLLIPLTLSKRAHSIFLLRLFNDPIAMLI
FYLSVIAFQIGGRKGWRLGCVLFSLALGVKMNILNSLPGLLVLLFQYRGIIGTVEGLSII
GLIQFLLPAPFFFSKRNPYLTRAYFTSAFDFSRQFLYEWTVNWRFISEEAFLSRERAVAL
LAGHFIVLGLFAAFKWSPVPGGTLRVLQKGFSSPLNQALEVSQIPAYHIPLVLFSANLIG
MLFARSLHYQFHSWYFHQLPFLLYSGAGWGNALTSVLIWVIVQYAWETAPSSISTSAALL
AGHVAMVFGLFVHGMKQLPSIQKVKTK

Enzyme Prediction     

EC	2.4.1.258:18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT58	40	416	3.1e-129	0.9945054945054945

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
398745	ALG3	7.61e-166	40	416	1	358	ALG3 protein. The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AFR94407.1|GT58	0.0	1	447	1	447
AUB24060.1|GT58	0.0	1	447	1	447
AAW46504.1|GT58	9.31e-306	1	447	1	447
KGB78711.1|GT58	1.51e-295	1	447	1	447
ADV21666.1|GT58	1.44e-293	1	447	1	447

PDB Hits     

CKF44_05142-t42_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P0CN92|ALG3_CRYNJ	1.65e-306	1	447	1	447	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=ALG3 PE=3 SV=1
sp|P0CN93|ALG3_CRYNB	4.74e-306	1	447	1	447	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=ALG3 PE=3 SV=1
sp|Q9C1K8|ALG3_NEUCR	2.19e-88	42	435	40	418	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=alg-3 PE=3 SV=1
sp|Q55F69|ALG3_DICDI	6.65e-85	35	431	18	422	Probable Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Dictyostelium discoideum OX=44689 GN=alg3 PE=3 SV=1
sp|Q8K2A8|ALG3_MOUSE	1.16e-83	28	442	35	432	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Mus musculus OX=10090 GN=Alg3 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999988	0.000030

TMHMM  Annotations     

Start	End
33	55
119	141
166	188
200	222
232	254
297	314
346	368
381	403
413	435