CAZyme Information: CKF44_03648-t42_1-p1

Basic Information

• Species: Cryptococcus neoformans
• Lineage: Arthropoda; Insecta; ; Eriococcidae; Cryptococcus; Cryptococcus neoformans
• CAZyme ID: CKF44_03648-t42_1-p1
• CAZy Family: GH17
• CAZyme Description: UDP-glucose:glycoprotein glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1582		176326.51	4.8077

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CneoformansKN99	6967	N/A	0	6967

• Gene Location: location=CP022322.1:433344-439213(+)

Full Sequence      

MRASTVALALAASALAASASPPVRVSLETGWAAPPLVLEILETVYDEYPSSYFQLLHLLS
SLPPDSTDESILSSTLDLIQAYSLLPSPSSLSTFHLALSLHSTAPRIEAQYNWYNSAVKG
QENKLGVEGCEGWVEWRGKGFCDVDGLKRDMEMSIEEGIHKINQEPHSLPFDRTFSSASS
TSFSPRAIFYYAPFASTSNELLGYLDHHASHYPEFTYTVRYQSPLSDAAKKPSSLSGWGA
EMALKKMDYLVVDDRATGKITFQNEGDEVARNESNIFAHVFGNDPWSDQATPLTATEIRD
IGLKAATLIMSSDDPISALTHLSQDFPKYSAALAHQVEVPEDIQSKGRTIAVRGKAKEAI
YINGKPFDRDLNPYTLLKALRDERQLTISLTSLGLTPKQSIDILADPVVGQGQIEDDMGE
GLVDASDRIEGGDVITYWNDIEKDKRYHNWPIHPQGYMRPVYPGQFHNVRRNTFNLIFAL
DLSRISSLELIVHSISTMIQRGLPIRFGIVPVFEPEQQDDISLQMAKVFWYSVKTFGRSS
TRDFFAAIIDAIPRQLNNPAPQVNDDVLRKGYDALSATSKKASLAFDDVLTSDDWDHHIE
KAGNYLKRLLITKKDTENGGMFMNGRFTPNAPTWPNIVTQEMQSQLAFIQEQVMLDAIPE
DISTMFYDLPATSKRRSSLVIPVGDNKLKVFNLVDLFKNEGIEGKLSGEFVYPDGERGTP
ISMWIIGDLDSPEGLEIVKNGLQHLQTPQCASRLGFIHVPPASRHSSCPAGQYCFSTVLY
QILSQNALSLTKPSDLLELISEVQHSVKTNLEKAGEINVGNQEVDDFGITFTLSPEDQQK
YFEAKPLHSMTFGGWVAGDTAAASEFWKAGTQIAGKLAITDGVHILVNGRLVGPITPVTF
PLDDFEALEAYEHRKRVKPIIDVLKTMYDDITAFDRPTLANLISKASSVVTAAYKPLDGE
GIFAPAQSTRTRYYERLDDGSTSFKLGDEDMSLLKVAVVVNPLSEQAQKWSPLIQTLSEM
DHVFVSVYLEPEALMEEVKLKRFYRTSVPSRLTFDVDGAAIAPGLTFNDLPSNPIYTLGL
DTPPSWIVSPRTSPYDLDNLLLSSTSSPVAVTFQLKQLLIEGHARESGNIPPRGLQLQLK
TLDGDIAADTQVMANLGYLQFRATPGYYTLSIRPGRGEEVFNLESIGAEGWDSPSVGEVG
DGVSLSSFDGETIYPRFARKEGMEKADVLQESVAAPEGLAKQVYSRMKSIVGLSTKVTPA
KSEHADINIFTVASGLLYERFASIMILSVMKHTNSSVKFWFIENFLSPTFITFIPKLAEE
YGFQYEFVTYKWPHWLRAQTEKQRIIWAYKILFLDVLFPMSLDKVIFVDADQIVRTDMKE
LVDVDLHGRVYGYAPMGNSRKEMEGFRFWKSGYWKEALRGRPYHISALYVVDLKKFRQLA
TGDRLRGQYHALSADPNSLANLDQDLPNSMQDQIPIWTLDQDWLWCQTWCSDESLATAKT
IDLCQNPLTKEPKLVRARQIPEWDAYDQEIAAFAARVSEEGEESGALAISVDYLASERHV
SGVEGKEGETEREAEGRIGDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1267	1514	2.3e-128	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
133054	GT8_HUGT1_C_like	0.0	1267	1514	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408203	Glyco_transf_24	0.0	1267	1533	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
408201	Thioredoxin_14	2.33e-77	423	680	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
132996	Glyco_transf_8	2.53e-55	1267	1514	1	246	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
399437	UDP-g_GGTase	6.96e-54	1121	1230	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUB22656.1|GT24	0.0	1	1582	1	1582
AFR93153.2|GT24	0.0	1	1582	1	1582
UOH81582.1|GT24	0.0	1	1582	1	1585
AAW41515.2|GT24	0.0	1	1582	1	1582
KGB74213.2|GT24	0.0	1	1582	1	1582

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	2.18e-295	18	1540	6	1454	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	2.38e-294	18	1540	6	1454	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TRT_A	2.38e-294	18	1540	6	1454	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	1.14e-260	20	1476	1	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	6.08e-213	675	1540	400	1220	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	1.47e-218	19	1543	18	1434	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	3.12e-201	20	1547	44	1536	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q6P5E4|UGGG1_MOUSE	7.86e-200	15	1530	39	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9JLA3|UGGG1_RAT	2.96e-199	15	1530	39	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	3.92e-198	18	1539	25	1513	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000343	0.999625	CS pos: 19-20. Pr: 0.7599

TMHMM  Annotations     

There is no transmembrane helices in CKF44_03648-t42_1-p1.