CAZyme Information: CKF44_03113-t42_1-p1

Basic Information

• Species: Cryptococcus neoformans
• Lineage: Arthropoda; Insecta; ; Eriococcidae; Cryptococcus; Cryptococcus neoformans
• CAZyme ID: CKF44_03113-t42_1-p1
• CAZy Family: GH152
• CAZyme Description: trehalose synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
734	CP022328.1|CGC10	82012.18	5.4129

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CneoformansKN99	6967	N/A	0	6967

• Gene Location: location=CP022328.1:80296-83337(-)

Full Sequence      

MPPGEPPKRRLSTTSSRQPTTIQDIFIGVGLQLSPQPDIPEGHEDPGRDLEYSAVIHDGT
GILDSETFHTTFFTYGKDEDGLAAEMKRVARDMLYLLRAIQTNRQVNIKMIAVAEPIPDE
LRAKNGVEFFPTLWLHMDAIPFITTPSTSIFTKLPAPSTIASGTAAVSAAVKHLHPATHS
ATTADVAPKDHHVQVDSDGQIRLCSILQYQQSSSEALWARFTALSRLLNANKVSIAFFSA
TPQGGGVALMRHALLRLWRMVGLPVKWFVPEGHPTVFNITKTKFHNVLQGVSPKEVEINE
TDKTWFELWTEQNYESFWSNGALDASVIVIDDPQLTALIPIIKKERPDAKIIFRSHIQIQ
SDLTDDPSTVQYRTWNYLFNFIKDVDLFLAHPVKFFVPKNVHENLPVLYMAPSTDPLDGL
NKMYGRASVRYYRQYFNQLSQAQCGVKIDWDRGYVCQIARFDPSKGIDILLKAYLEFRQK
LEECENPPLDNGPQLIIMGHGSIDDPDGSWVYEKIHDTLNSPGYELIHGDVAVVRAPPSD
ALLGCILQGAWVATQLSTREGFEVKVTEAINKRVPIIASDAGGIPLQVKEGKNGWIVPSG
DSAAVSDTLYKIYKGKLSVHRDLSEEKELDGKSDPNSVAQEWVGNFDEAYRKIHDDDGAT
SEDFWTVGNATRWMLLFAKLLDLKIDQTGEVNEQDVNVLKKLEKEKLPNKGETGGNVWHM
LMGDDMLKDEGALI

Enzyme Prediction     

EC	2.4.1.231:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT4	454	614	8.1e-25	0.86875

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340823	GT4_trehalose_phosphorylase	2.32e-175	234	649	1	378	trehalose phosphorylase and similar proteins. Trehalose phosphorylase (TP) reversibly catalyzes trehalose synthesis and degradation from alpha-glucose-1-phosphate (alpha-Glc-1-P) and glucose. The catalyzing activity includes the phosphorolysis of trehalose, which produce alpha-Glc-1-P and glucose, and the subsequent synthesis of trehalose. This family is most closely related to the GT4 family of glycosyltransferases.
223515	RfaB	5.70e-18	231	614	1	341	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
395425	Glycos_transf_1	2.62e-17	451	621	1	150	Glycosyl transferases group 1. Mutations in this domain of PIGA lead to disease (Paroxysmal Nocturnal haemoglobinuria). Members of this family transfer activated sugars to a variety of substrates, including glycogen, Fructose-6-phosphate and lipopolysaccharides. Members of this family transfer UDP, ADP, GDP or CMP linked sugars. The eukaryotic glycogen synthases may be distant members of this family.
340816	Glycosyltransferase_GTB-type	2.56e-12	382	597	52	235	glycosyltransferase family 1 and related proteins with GTB topology. Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. The structures of the formed glycoconjugates are extremely diverse, reflecting a wide range of biological functions. The members of this family share a common GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.
404563	Glyco_trans_1_4	3.73e-12	458	614	7	137	Glycosyl transferases group 1.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUB26242.1|GT4	0.0	1	734	1	734
AFR96337.1|GT4	0.0	1	734	1	734
UOH82745.1|GT4	0.0	1	734	1	734
AAW44824.1|GT4	0.0	1	734	1	734
ADV23288.1|GT4	0.0	1	734	1	734

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2X6Q_A	2.40e-36	234	626	42	385	Crystal structure of trehalose synthase TreT from P.horikoshi [Pyrococcus horikoshii],2X6Q_B Crystal structure of trehalose synthase TreT from P.horikoshi [Pyrococcus horikoshii],2X6R_A Crystal structure of trehalose synthase TreT from P.horikoshi produced by soaking in trehalose [Pyrococcus horikoshii],2X6R_B Crystal structure of trehalose synthase TreT from P.horikoshi produced by soaking in trehalose [Pyrococcus horikoshii]
2XA2_A	1.49e-35	234	626	42	385	Crystal structure of trehalose synthase TreT mutant E326A from P. horikoshii in complex with UDPG [Pyrococcus horikoshii],2XA2_B Crystal structure of trehalose synthase TreT mutant E326A from P. horikoshii in complex with UDPG [Pyrococcus horikoshii],2XA9_A Crystal structure of trehalose synthase TreT mutant E326A from P. horikoshii in complex with UDPG [Pyrococcus horikoshii],2XA9_B Crystal structure of trehalose synthase TreT mutant E326A from P. horikoshii in complex with UDPG [Pyrococcus horikoshii],2XMP_A Crystal structure of trehalose synthase TreT mutant E326A from P. horishiki in complex with UDP [Pyrococcus horikoshii],2XMP_B Crystal structure of trehalose synthase TreT mutant E326A from P. horishiki in complex with UDP [Pyrococcus horikoshii]
2XA1_A	2.03e-35	234	626	42	385	Crystal structure of trehalose synthase TreT from P.horikoshii (Seleno derivative) [Pyrococcus horikoshii],2XA1_B Crystal structure of trehalose synthase TreT from P.horikoshii (Seleno derivative) [Pyrococcus horikoshii]
6ZJ4_AAA	6.77e-30	233	604	27	351	Chain AAA, Trehalose phosphorylase/synthase [Thermoproteus uzoniensis],6ZJ7_AAA Chain AAA, Trehalose phosphorylase/synthase [Thermoproteus uzoniensis 768-20],6ZJH_AAA Chain AAA, Trehalose phosphorylase/synthase [Thermoproteus uzoniensis 768-20],6ZMZ_AAA Chain AAA, Trehalose phosphorylase/synthase [Thermoproteus uzoniensis],6ZN1_AAA Chain AAA, Trehalose phosphorylase/synthase [Thermoproteus uzoniensis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A6YRN9|TREPH_PLEPU	3.60e-102	193	711	236	739	Trehalose phosphorylase OS=Pleurotus pulmonarius OX=28995 GN=TP PE=2 SV=1
sp|O75003|TREPH_GRIFR	1.40e-97	147	676	188	686	Trehalose phosphorylase OS=Grifola frondosa OX=5627 PE=1 SV=1
sp|Q9UV63|TREPH_PLESA	8.25e-97	193	676	236	706	Trehalose phosphorylase OS=Pleurotus sajor-caju OX=50053 GN=TP PE=1 SV=1
sp|Q7LYW5|TRET_THELN	1.53e-39	229	632	35	389	Trehalose synthase OS=Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) OX=523849 GN=treT PE=1 SV=1
sp|Q9HH00|TRET_PYRFU	1.53e-39	229	632	35	389	Trehalose synthase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=treT PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000055	0.000002

TMHMM  Annotations     

There is no transmembrane helices in CKF44_03113-t42_1-p1.