CAZyme Information: CKF44_02850-t42_1-p1

Basic Information

• Species: Cryptococcus neoformans
• Lineage: Arthropoda; Insecta; ; Eriococcidae; Cryptococcus; Cryptococcus neoformans
• CAZyme ID: CKF44_02850-t42_1-p1
• CAZy Family: GH13
• CAZyme Description: glucan endo-1,3-alpha-glucosidase agn1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
896		94147.33	3.9801

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CneoformansKN99	6967	N/A	0	6967

• Gene Location: location=CP022323.1:615950-618792(-)

Full Sequence      

MAPIIEAAIILLALAGNVVEAKPLRTPGRHAPPGVANVLASSKRSLHNLLARYYGTAHGL
SKPPSLPTKRDTSLPDGWSTFGCVAESYDERLLQGFAFSSSSLTPLLCVTQCTKLGYTMA
GTEYGDECYCGDNFNGNGGGMAPSSICNMPCEGDTSEMCGNSWYLNLYMYNSSALPLCGG
PTSTVSAPAEETSSLLSTYYSSFESSVTASSSLASASSTDAAISANSSTSVMSASATTTV
SSGTIEATGSETNSVSGDTSLAPTTSSAATSTSTSPVHEDSDDSSEWNALGCGLDSEDRI
LSSYFISLDNMTVDSCLTICENRGYVFAGLQFSDECYCGNSLLQSTSYDSTRCDMSCAGD
SEDTCGGSWAIELFELISSPSSTCTDSLSTETATTVLATSTTSGFNTASTTAIASSTNSA
SSSVIVTSKYTGETVSSGQATEATTVTESTTGSETANVSVTSESVIAPTSTTSTESTASA
IPTSVPSSSSTHQVWAHYMVGNTYPYTASNWASDISAALAAGIDGFALNIGSDNWQPARV
ADAYSAAASTGFKMFLSLDMTVLSCSSSSDAANLVSIVQKYANAAAQATYEGKVLVSTFA
GSDCAFSWQSDFVDVLSSAGIDIFFIPSIFSDVSTFSSNIWMDGELNWNSGWPMGAEDIT
VASDETYMTALGDKVYMPAVSPFFYTHFGANSWNKNWLYRSDDWLYCTRWEQLIAMRESV
KMTEILTWNDYGESSYIGPIQGALPAGSETFVDGFTHTGLYSLTNYYATAFKTGAYPMIT
EDEIIMWARPHPHDATASSDPIGRPTGWSYTDDYLYAVVLTTGSATVTLTSGSITETFSV
SAGLTKLKVSLSEGSISGSISRSGRTVASYNAGSAFTYTTSPITYNFNYFVGSSSS

Enzyme Prediction     

EC	3.2.1.59:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH71	494	870	2.3e-124	0.9946666666666667

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397634	Glyco_hydro_71	1.49e-158	494	850	1	352	Glycosyl hydrolase family 71. Family of alpha-1,3-glucanases.
211418	GH71	1.59e-103	494	774	7	283	Glycoside hydrolase family 71. This family of glycoside hydrolases 71 (following the CAZY nomenclature) function as alpha-1,3-glucanases (mutanases, EC 3.2.1.59). They appear to have an endo-hydrolytic mode of enzymatic activity and bacterial members are investigated as candidates for the development of dental caries treatments.The member from fission yeast, endo-alpha-1,3-glucanase Agn1p, plays a vital role in daughter cell separation, while Agn2p has been associated with endolysis of the ascus wall.
211414	GH99_GH71_like	2.01e-24	494	740	1	254	Glycoside hydrolase families 71, 99, and related domains. This superfamily of glycoside hydrolases contains families GH71 and GH99 (following the CAZY nomenclature), as well as other members with undefined function and specificity.
396406	WSC	1.46e-20	290	367	1	80	WSC domain. This domain may be involved in carbohydrate binding.
396406	WSC	6.95e-18	82	160	2	79	WSC domain. This domain may be involved in carbohydrate binding.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AFR93842.1|GH71	0.0	1	896	1	896
AUB23423.1|GH71	0.0	1	896	1	896
UOH80041.1|GH71	0.0	1	896	1	896
AAW42417.1|GH71	0.0	1	896	1	881
ADV21435.1|GH71	0.0	1	896	1	848

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FWW_B	6.27e-06	309	377	111	181	Wnt modulator Kremen in complex with DKK1 (CRD2) and LRP6 (PE3PE4) [Homo sapiens]
7BZT_E	8.78e-06	309	377	120	190	Chain E, KRM1 [Homo sapiens],7BZU_E Chain E, KRM1 [Homo sapiens]
6SNW_E	8.86e-06	309	377	123	193	Chain E, Kremen protein 1 [Homo sapiens]
5FWS_A	9.50e-06	309	377	151	221	Wnt modulator Kremen crystal form I at 1.90A [Homo sapiens],5FWT_A Wnt modulator Kremen crystal form I at 2.10A [Homo sapiens],5FWU_A Wnt modulator Kremen crystal form II at 2.8A [Homo sapiens],5FWV_A Wnt modulator Kremen crystal form III at 3.2A [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O13716|AGN1_SCHPO	1.89e-87	488	892	18	420	Glucan endo-1,3-alpha-glucosidase agn1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=agn1 PE=1 SV=2
sp|B6GX22|MU71A_PENRW	2.84e-50	480	893	24	429	Mutanase Pc12g07500 OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=PCH_Pc12g07500 PE=1 SV=1
sp|O94510|AGN2_SCHPO	5.50e-50	480	850	3	381	Ascus wall endo-1,3-alpha-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=agn2 PE=1 SV=2
sp|D4AUF1|WSCD1_ARTBC	1.59e-15	76	380	61	407	WSC domain-containing protein ARB_07867 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_07867 PE=1 SV=1
sp|D4AUF4|WSCD2_ARTBC	7.31e-13	71	168	519	612	WSC domain-containing protein ARB_07870 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_07870 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000257	0.999708	CS pos: 21-22. Pr: 0.9774

TMHMM  Annotations     

There is no transmembrane helices in CKF44_02850-t42_1-p1.