CAZyme Information: CIHG_08353-t26_1-p1

Basic Information

• Species: Coccidioides immitis
• Lineage: Ascomycota; Eurotiomycetes; ; Onygenaceae; Coccidioides; Coccidioides immitis
• CAZyme ID: CIHG_08353-t26_1-p1
• CAZy Family: GT34
• CAZyme Description: acid trehalase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1050		114564.00	4.9800

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CimmitisH538-4	10724	396776	186	10538

• Gene Location: location=DS017022:44842-48172(+)

Full Sequence      

MFTNTLFAGLLFAGQVLGVAHSHGSKASKILDRNILSQRLDKRESNIQAPIYPTKFSGVT
WDNTNWRLTTTVLDQGHYQSRGSIANGYLGISVAAVGPFFELDVPVNGDVINGWPLFSRR
QTFATISGFHDLQPETNGTNFPWMNQYGGESVISGVPHWSGMILDLGQDGYLDASVENST
ISDFVSTLDMKAGTLTWKYKWTPAGRNDSFDIAYQLFAHKLHVNQAVVHMEITPSTNIDA
TVVNILDGYSAVRTQFAGSGTDGQAIYSSVRPDGISNVTAYIYAQMAGTDEVDMSTLSLI
TDKPYIRRNDSTIAQAVSVRLRAGVTTGVTKFVGAASTDGFADPRLVAKDSCAKALNNGF
ETSLRSHIDEWATVFPDDSVDSFSLPDTGHLPLDNHIIESAITAVANPYYLLQTTVSTNA
RKAVNDAPINRGSMAVGGLTSDSYAGLVFWDADIWMQPGFVPAFPEAAQSFTNYRVDKYG
QALANAQTAFTSSKNRTHISPDAAIYSWTSGRFGNCTGTGPCFDYQYHLNGDIGLQLINN
WVTTGDTEYFKSDLFPLYNSIATLFADVLERNGSKWTLTNMTDPDEYANHVDAGGFTMPM
IASTLIYANSFRQMFGLEQNQTWNDMAENVLISRDPSSSITLEYTTMNGSTQVKQADIVL
NTFPLRYTENYSPENALLDLDYYAAKQSPDGPAMTYAIFSIVANEVSPSGCSAYTYAQYS
FSPYVRAPFFQFSEQLVDDWLTNGGTHPAFPFLTGKGGANQVVLFGYLGYRLLPDSVLHI
DPNLPPQIPHINYRTFYWHGWPIKAESNYTHTTIRRAQHRTARPTADQRFADAPIPVHVG
PESNVTVYSLPPSGDLVIQNRQIGSIDTFPGNLVQCQPVSSPHEYLPGQFPIAAVDGAAS
TKWQPTRANMTASLTVTFPESALSSSISGFGFDWAQAPPQNAKVLLHDFPLPPAVDIFAE
SPPGSIVVADLSEIAVSDPYKPNVTDLNKIMRYKGNTTNVTLSSPVPVTKFATLLISGNQ
GLGDAEIKAGNGTGATVAEWSILGERTNRP

Enzyme Prediction     

EC	3.2.1.28:5	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH65	408	772	1.3e-78	0.9811827956989247

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224471	ATH1	9.10e-62	65	794	10	725	Trehalose and maltose hydrolase (possible phosphorylase) [Carbohydrate transport and metabolism].
397616	Glyco_hydro_65N	8.61e-43	74	338	9	240	Glycosyl hydrolase family 65, N-terminal domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. This domain is believed to be essential for catalytic activity although its precise function remains unknown.
281612	Glyco_hydro_65m	7.88e-20	407	720	3	345	Glycosyl hydrolase family 65 central catalytic domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. The central domain is the catalytic domain, which binds a phosphate ion that is proximal the the highly conserved Glu. The arrangement of the phosphate and the glutamate is thought to cause nucleophilic attack on the anomeric carbon atom. The catalytic domain also forms the majority of the dimerization interface.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS73854.1|CBM32|GH65	0.0	2	1043	5	1062
QSS66944.1|GH65	0.0	7	1043	10	1062
UKZ51236.1|GH65	0.0	46	1044	45	1042
UKZ77068.1|GH65	0.0	46	1044	45	1042
QYS98630.1|CBM32|GH65	0.0	26	1050	29	1052

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7FE3_A	1.07e-38	66	769	24	639	Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_A Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101]
3WIQ_A	4.06e-15	433	800	326	722	Crystal structure of kojibiose phosphorylase complexed with kojibiose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_A Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_B Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_C Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_D Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903]
4KTP_A	1.68e-12	413	845	295	751	Chain A, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTP_B Chain B, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10]
4KTR_A	3.82e-12	413	845	295	751	Chain A, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_B Chain B, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_C Chain C, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_D Chain D, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_E Chain E, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_F Chain F, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_G Chain G, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_H Chain H, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4B5H9|ATC1_ARTBC	0.0	43	1043	44	1051	Cell wall acid trehalase ARB_03719 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_03719 PE=1 SV=1
sp|P78617|TREA_EMENI	0.0	52	1050	50	1044	Acid trehalase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=treA PE=3 SV=1
sp|Q5AAU5|ATC1_CANAL	1.43e-127	78	944	121	964	Cell wall acid trehalase ATC1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ATC1 PE=1 SV=2
sp|P48016|ATH1_YEAST	5.66e-116	19	944	75	1034	Periplasmic acid trehalase ATH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ATH1 PE=1 SV=1
sp|N1P212|ATH1_YEASC	5.66e-116	19	944	75	1034	Periplasmic acid trehalase ATC1 OS=Saccharomyces cerevisiae (strain CEN.PK113-7D) OX=889517 GN=CENPK1137D_1727 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.065308	0.934666	CS pos: 22-23. Pr: 0.6566

TMHMM  Annotations     

There is no transmembrane helices in CIHG_08353-t26_1-p1.