CAZyme Information: CGB_J2210C-t26_1-p1

Basic Information

• Species: Cryptococcus gattii VGI
• Lineage: Arthropoda; Insecta; ; Eriococcidae; Cryptococcus; Cryptococcus gattii VGI
• CAZyme ID: CGB_J2210C-t26_1-p1
• CAZy Family: GT32
• CAZyme Description: putative glucosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
623		68631.20	4.3793

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CgattiiWM276	6580	367775	15	6565

• Gene Location: location=CP000295:413964-416244(-)

Full Sequence      

MLSSSRQGREPLLQSAQNPGQSQRLSDSSLTSFDHQSMSYRGSHNEASARSYGSSSSNRF
GPTASLASGASAAVLASNSGGFSDELPQQAPSQAHIQDWHDADDLDDHLHTFTSQDRRDL
ATPFDITSARGWANALTLGILAGGGVMLFAGYPIISFYYGDSNSAGANTSGYNLGGINSS
GQYPEIPGLPTLIDADTPEWAYSRTGFDGDEWTLVFSDEFNKEGRTFFEGDDPFWTGMDI
HYWPTGDFEWLDPSAVTTADGHLVITMTQEPIHDLNFKSGMLQSWNKLCFNKHAYFEFSA
SLPGQTAYGGFWPGIWTMGNLGRPGYGASTEGMWPYTYDSCDVGTLPNQTYVNGTGPPAT
LTTGADGSPLSYLPGQRCSACTCPGEDHPGPVNTKGRAAPEIDIVEAQIIIAESRGEVSQ
SFQVAPYDDHYQSNNSTTNYKHYDTDLTYWNTYLGGPFQQAVSSLTRLPRNIYWDQPGDS
KQFAVFAMEYQAFPDARDQGYITWWADNKTSWTMYADAVAENPRTGIGRRIIPEEPMAMI
VNLHMSNNFQAVDFANLKWPNYFRIDYVRVYQKPDQISIGCDPDDYPTADYIARHAEVYS
NPNLTTWAAAGYTFPKSSLKGEC

Enzyme Prediction     

No EC number prediction in CGB_J2210C-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	218	571	1.9e-137	0.9969325153374233

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	0.0	116	623	24	500	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	2.12e-167	214	572	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	2.53e-42	192	585	29	355	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	1.68e-18	216	571	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185687	GH16_beta_agarase	1.91e-04	208	312	15	117	Beta-agarase, member of glycosyl hydrolase family 16. Beta-agarase is a glycosyl hydrolase family 16 (GH16) member that hydrolyzes the internal beta-1,4-linkage of agarose, a hydrophilic polysaccharide found in the cell wall of Rhodophyceaea, marine red algae. Agarose is a linear chain of galactose units linked by alternating L-alpha-1,3- and D-beta-1,4-linkages that are additionally modified by a 3,6-anhydro-bridge. Agarose forms thermo-reversible gels that are widely used in the food industry or as a laboratory medium. While beta-agarases are also found in two other families derived from the sequence-based classification of glycosyl hydrolases (GH50, and GH86) the GH16 members are most abundant. This domain adopts a curved beta-sandwich conformation, with a tunnel-shaped active site cavity, referred to as a jellyroll fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADV24708.1|GH16_2	0.0	1	623	1	623
KGB80226.3|GH16_2	0.0	1	623	1	623
UOH81139.1|GH16_2	0.0	1	623	1	622
AUB25004.1|GH16_2	0.0	1	623	1	622
AFR95233.2|GH16_2	0.0	1	623	1	622

PDB Hits     

CGB_J2210C-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P87024|SKN1_CANAX	2.00e-134	146	621	293	736	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P32486|KRE6_YEAST	9.77e-134	101	623	214	711	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P33336|SKN1_YEAST	5.58e-133	106	623	259	763	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P87023|KRE6_CANAX	8.93e-130	183	621	321	739	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|O13941|YEPB_SCHPO	5.07e-125	192	623	218	627	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000060	0.000000

TMHMM  Annotations     

Start	End
132	154