CAZyme Information: CGB_D2280C-t26_1-p1

Basic Information

• Species: Cryptococcus gattii VGI
• Lineage: Arthropoda; Insecta; ; Eriococcidae; Cryptococcus; Cryptococcus gattii VGI
• CAZyme ID: CGB_D2280C-t26_1-p1
• CAZy Family: GH15
• CAZyme Description: alpha-1,3-mannosyltransferase, putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
447	CP000289|CGC10	50545.66	10.1384

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CgattiiWM276	6580	367775	15	6565

• Gene Location: location=CP000289:534045-535682(-)

Full Sequence      

MSDRNPSSPALGQRKGLVSHGVDLVRALFFDRRYFWHTAFLLFLGEAALSLLVIWKIPYT
KIDWPAYMQQVDMFLAGERDYSKIEGETGPLVYPALHLYIYTAFHRLLPSIENVRPAQFV
FLVFYLATFLAVCTIYYLAGRPSNGGRHFPQMLLIPLTLSKRAHSIFLLRLFNDPIAMLI
FYLSVIALQIGGRKGWRLGCVLFSLALGVKMNILNFLPGLLVLLFQYRGIVGTMEGLSII
GLIQFILPAPFFFAKSDPHLLKAYFTSAFDFSRQFLYEWTVNWRFISEEAFLSRERAVAL
LAGHLTVLGLFAAFKWSPVPGGTLKVLQKGFSNPLNQALEVSQVPAYHIPLVLFSANLIG
MLFARSLHYQFLSWYFHQLPFLLYSGAGWGNTLTSVIIWVAVQYAWETAPSTINTSIALL
AGHGAMVLGLFFHGMKQLSSKQKFKTK

Enzyme Prediction     

EC	2.4.1.258:18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT58	40	416	2.3e-132	0.9945054945054945

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
398745	ALG3	1.09e-171	40	416	1	358	ALG3 protein. The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADV21666.1|GT58	0.0	1	447	1	447
KGB78711.1|GT58	1.28e-317	1	447	1	447
AAW46504.1|GT58	5.49e-298	1	447	1	447
AFR94407.1|GT58	9.69e-292	1	447	1	447
AUB24060.1|GT58	9.69e-292	1	447	1	447

PDB Hits     

CGB_D2280C-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P0CN93|ALG3_CRYNB	4.15e-300	1	447	1	447	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=ALG3 PE=3 SV=1
sp|P0CN92|ALG3_CRYNJ	9.76e-299	1	447	1	447	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=ALG3 PE=3 SV=1
sp|Q55F69|ALG3_DICDI	2.37e-85	35	432	18	423	Probable Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Dictyostelium discoideum OX=44689 GN=alg3 PE=3 SV=1
sp|Q9C1K8|ALG3_NEUCR	4.21e-85	42	443	40	438	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=alg-3 PE=3 SV=1
sp|Q92685|ALG3_HUMAN	3.60e-82	26	431	33	421	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Homo sapiens OX=9606 GN=ALG3 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000039	0.000013

TMHMM  Annotations     

Start	End
33	55
119	141
166	188
200	222
232	254
297	314
346	368
381	403
413	435