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CAZyme Information: CCE29618.1

You are here: Home > Sequence: CCE29618.1

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Claviceps purpurea
Lineage Ascomycota; Sordariomycetes; ; Clavicipitaceae; Claviceps; Claviceps purpurea
CAZyme ID CCE29618.1
CAZy Family GH18
CAZyme Description Related to acid beta-fructofuranosidase [Source:UniProtKB/TrEMBL;Acc:M1W945]
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
566 CAGA01000015|CGC1 63414.39 6.2003
Genome Property
Genome Version/Assembly ID Genes Strain NCBI Taxon ID Non Protein Coding Genes Protein Coding Genes
FungiDB-61_Cpurpurea20.1 8979 1111077 156 8823
Gene Location Start: 406685; End:408385  Strand: +

Full Sequence      Download help

MAYSRYRPIS  HFIAPHSWSN  DPCGAVYVPE  SQEYLLCYQW  NPGTTEGGNC  AWGMAKSKDL60
VTWTDCMPAI  WNGTSYDSVG  VFSGCIVSRL  VEGKRVLYLY  YTSVSALPIH  WSKPYIKGCE120
TQSMAVSTDL  GRTWVRHESN  PLIGEAPKKA  ATTGWRDPFV  SPSASLSRLL  GLPREHDFMM180
MSSGERGRGP  QLHLYGSEDL  HSWNYISTVL  DVQLHSVISE  TSNLKWGMNF  ECASFFTIGQ240
TDYIIVGIEE  SEESTNHNAH  YVLWMAGEFH  LDEKNLPTFK  IRGHEVLDHG  ILYAAHLFRD300
ESDRLLQLGW  ADESAKKHIV  QDQGWAGCLG  HPRELTQITR  PIHPCARSWH  EWTIDESTGT360
MSTLGIRPAI  QVEALRVGPP  TAGPEALTQF  KQLSSENFEV  RAKFSNVTGR  ERFVFHVLRS420
PDGHETTSLV  FDLKTQTISV  DRSKSSLKQL  GTTSPDSGHM  RLLPGEDLDV  RIFVDVSIIE480
IYANDRFALT  SRVYPSLETS  KQTVYDFGAF  DLGNVEMVYW  QSLKQAWPER  GSGVEMGAGF540
LSRSALEKSR  QVATASEVHV  QTEAAS566

Enzyme Prediction      help

EC 3.2.1.26:2

CAZyme Signature Domains help

Created with Snap2856841131411691982262542833113393673964244524815095371281GH32
Family Start End Evalue family coverage
GH32 11 336 7.1e-68 0.9556313993174061

CDD Domains      download full data without filtering help

Created with Snap28568411314116919822625428331133936739642445248150953717339GH32_XdINV-like11485Glyco_3218336GH32_FFase1496SacC11336Glyco_hydro_32N
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
350133 GH32_XdINV-like 1.12e-123 17 339 1 337
glycoside hydrolase family 32 protein such as Xanthophyllomyces dendrorhous beta-fructofuranosidase (Inv;Xd-INV;XdINV). This subfamily of glycosyl hydrolase family GH32 includes fructan:fructan 1-fructosyltransferase (FT, EC 2.4.1.100) and beta-fructofuranosidase (invertase or Inv, EC 3.2.1.26), among others. These enzymes cleave sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. Xanthophyllomyces dendrorhous beta-fructofuranosidase (XdINV) also catalyzes the synthesis of fructooligosaccharides (FOS, a beneficial prebiotic), producing neo-FOS, making it an interesting biotechnology target. Structural studies show plasticity of its active site, having a flexible loop that is essential in binding sucrose and beta(2-1)-linked oligosaccharide, making it a valuable biocatalyst to produce novel bioconjugates. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
214757 Glyco_32 7.88e-66 11 485 1 436
Glycosyl hydrolases family 32.
350110 GH32_FFase 2.22e-64 18 336 2 280
Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
224536 SacC 1.09e-46 1 496 23 460
Sucrose-6-phosphate hydrolase SacC, GH32 family [Carbohydrate transport and metabolism].
395193 Glyco_hydro_32N 4.35e-43 11 336 1 296
Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure.

CAZyme Hits      help

Created with Snap2856841131411691982262542833113393673964244524815095371566QPG98192.1|GH321565ANH56454.1|GH321566UKZ94734.1|GH321566QUC20479.1|GH321534UNI23891.1|GH32
Hit ID E-Value Query Start Query End Hit Start Hit End
QPG98192.1|GH32 1.04e-310 1 566 1 569
ANH56454.1|GH32 3.34e-296 1 565 1 563
UKZ94734.1|GH32 6.61e-287 1 566 37 612
QUC20479.1|GH32 1.04e-282 1 566 24 589
UNI23891.1|GH32 3.51e-218 1 534 13 555

PDB Hits      download full data without filtering help

Created with Snap28568411314116919822625428331133936739642445248150953735376S82_A35375ANN_A35375FK7_A35375NSL_A35375FIX_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
6S82_A 3.30e-49 3 537 62 648
Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],6S82_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma]
5ANN_A 3.30e-49 3 537 62 648
Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],5ANN_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma]
5FK7_A 4.03e-48 3 537 60 646
Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],5FK7_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma],5FK8_A Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],5FK8_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma],5FKB_A Chain A, BETA-FRUCTOFURANOSIDASE [Phaffia rhodozyma],5FKB_B Chain B, BETA-FRUCTOFURANOSIDASE [Phaffia rhodozyma],5FKC_A Chain A, BETA-FRUCTOFURANOSIDASE [Phaffia rhodozyma],5FKC_B Chain B, BETA-FRUCTOFURANOSIDASE [Phaffia rhodozyma],5FMC_A Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],5FMC_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma]
5NSL_A 4.13e-48 3 537 62 648
Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],5NSL_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma],5O47_A Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],5O47_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma],6FJE_A Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],6FJE_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma],6FJG_A Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],6FJG_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma],6S2G_A Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],6S2G_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma],6S2H_A Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],6S2H_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma],6S3Z_A Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],6S3Z_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma]
5FIX_A 4.13e-48 3 537 62 648
Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],5FIX_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma],5FMB_A Chain A, BETA-FRUCTOFURANOSIDASE [Phaffia rhodozyma],5FMB_B Chain B, BETA-FRUCTOFURANOSIDASE [Phaffia rhodozyma],5FMD_A Chain A, Beta-fructofuranosidase [Phaffia rhodozyma],5FMD_B Chain B, Beta-fructofuranosidase [Phaffia rhodozyma]

Swiss-Prot Hits      download full data without filtering help

Created with Snap2856841131411691982262542833113393673964244524815095376505sp|P05656|SACC_BACSU7506sp|P49175|INV1_MAIZE6505sp|P40714|CSCA_ECOLX7496sp|Q43857|INVA_VICFA6526sp|Q70XE6|6FEH_BETVU
Hit ID E-Value Query Start Query End Hit Start Hit End Description
sp|P05656|SACC_BACSU 1.61e-24 6 505 34 493
Levanase OS=Bacillus subtilis (strain 168) OX=224308 GN=sacC PE=1 SV=1
sp|P49175|INV1_MAIZE 2.55e-20 7 506 125 630
Beta-fructofuranosidase 1 OS=Zea mays OX=4577 GN=IVR1 PE=3 SV=1
sp|P40714|CSCA_ECOLX 2.29e-19 6 505 24 460
Sucrose-6-phosphate hydrolase OS=Escherichia coli OX=562 GN=cscA PE=3 SV=1
sp|Q43857|INVA_VICFA 3.92e-18 7 496 108 596
Acid beta-fructofuranosidase OS=Vicia faba OX=3906 GN=VCINV PE=2 SV=1
sp|Q70XE6|6FEH_BETVU 6.39e-18 6 526 55 582
Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI CS Position
0.999867 0.000183

TMHMM  Annotations      help

There is no transmembrane helices in CCE29618.1.