CAZyme Information: CC1G_03121-t26_1-p1

Basic Information

• Species: Coprinopsis cinerea
• Lineage: Basidiomycota; Agaricomycetes; ; Psathyrellaceae; Coprinopsis; Coprinopsis cinerea
• CAZyme ID: CC1G_03121-t26_1-p1
• CAZy Family: AA9
• CAZyme Description: beta-mannosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
978	AACS02000008|CGC4	110090.73	5.6953

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Ccinereaokay7-130	13657	240176	301	13356

• Gene Location: location=AACS02000008:2334171-2337691(-)

Full Sequence      

MASSATVCTSLSGKWFWKQRNPGIGLQEELNNLPRGPEEGLKLTDPADRESIVSSWNLTK
GGDVPSEIHVELLGAGIIPHPYRGFGIADPEWLYACSFSLPAEPEQAARVKSAILTFEGL
DTISDVYLNGEKVIQADNMFRGYQYHLPNASVGVPLKGDNTLLLHFASAKALAKQLEAEF
GKVRAGSTNLGDPSRVYVRKAQYGWRWDWGPELMTCGPYRPITLTTIYHSVYVEDIWTKA
WVSPIASPNGTITSYLPSLAVDVTVAQVSEFEDRYCVEIVLLDPDSHNEIRTLKSAKLGL
PFSAANATRPDGHQKHAVVQLEWPDLRALNVQPWWPAGMGAQKLYLVEVRLLYHRADDEA
NTPMVVQSIKKRVGFRSARLVENPLVEPDQYGKGTTFFFEINGERVFVGGSNWIPADNFL
TTLSEQRYREWLTLVRDGNQTMVRVWGGGIYEPDIFYDICDELGLLVWQDFQFACGVYPG
VSSAAPDVAPKSYPAFVLNVKKEAEYNVKRLRHHPSIVVWCGNNEDYQMVLQWGDVPHLP
ATVLYEDILPSIVKALTSFTISFGLSCQDVEASYVRGSPYGGQGWDTADPTVGDVHQWNV
WGGKELPYQEYGKLGGRFISEFGIPSFPALKTVQYWMEGADQSEWYAQSKAMAQHIRAGS
YERRFAILMNENFRNVASSDFDRYRFNTQVMQSEAVSYAYRVWRREWRGKGKEYCGGVLV
WQFNDCWPVASWALVDYFMRPKPVYHTIRRSLEPIAVNILREVVQNRANDRPKQFYEYGA
TRSLGANLHIWAVNTTRKAVKVKLDIQYFDLESDWTSRNGVTSEFTLPPNQSTDLLALVC
PSPPTSRLRDTVGPGIDGSGTVVVLARLLDPSTNLVLARFVDWPQPFRYLHIPDPQVDLN
VLDVDLTTGDATIQLSVAKPAKCLWLEAEDEQALDGRTDQEREVKWNDNALDIVPGDSQV
VVARGLGKRKVKVTWLKD

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	8	835	4.1e-85	0.6875

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	4.82e-62	62	749	32	627	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	9.91e-14	787	889	2	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
397119	Glyco_hydro_2_C	0.006	456	527	64	137	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRV98035.1|GH2	2.32e-290	10	976	7	919
QRW12613.1|GH2	3.23e-283	10	976	7	917
QRW25365.1|GH2	6.07e-269	10	961	7	858
QRV83983.1|GH2	5.20e-268	10	976	7	895
CCA67509.1|GH2	1.11e-257	11	976	8	855

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	3.69e-109	33	759	15	660	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	1.56e-94	54	805	13	719	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	1.62e-94	54	805	15	721	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	1.65e-94	54	805	15	721	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	4.21e-94	54	805	13	719	Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|I2C092|MANBB_THETO	7.16e-185	65	976	32	852	Beta-mannosidase B OS=Thermothelomyces thermophilus OX=78579 GN=man9 PE=1 SV=1
sp|Q0CCA0|MANBB_ASPTN	4.23e-176	49	966	6	829	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
sp|A2QYN2|MANBB_ASPNC	1.22e-175	64	976	32	842	Beta-mannosidase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndB PE=3 SV=1
sp|Q4WAH4|MANBB_ASPFU	4.92e-175	64	966	32	831	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
sp|B8NW36|MANBB_ASPFN	6.73e-175	64	976	32	842	Beta-mannosidase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000019	0.000000

TMHMM  Annotations     

There is no transmembrane helices in CC1G_03121-t26_1-p1.