CAZyme Information: CAP83026:RNA-p1

Basic Information

• Species: Penicillium rubens
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Penicillium; Penicillium rubens
• CAZyme ID: CAP83026:RNA-p1
• CAZy Family: CE12
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
800		87583.25	4.3725

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PrubensWisconsin54-1255	14021	500485	1464	12557

• Gene Location: location=Pc00c15:322960-325426(-)

Full Sequence      

MNWFALLAMGVTFQVIHQANAKAVFAHFMVTNSENYTASDWQSDMGLAKDAHIDAFALNM
AWEDNTNSASVEMAFAAANAKGFKLFFSFDYAGNGPWDKGVVTSMIQKYGSNSAYFQYNG
RPFVSTFEGPGSADDWVTIKSETNCFFMPDWSSVGAKPAVELANGVADGLFSWSAWPWGN
HTMDTYTDASYIQYLDGKPYMIAISPWFYTNLPGYNKNWLWKGDSLWFDRWQELFGLDPM
PEFVQIISWNDYGESHYIGPIHDNAMAAFDIGEAPYNYVMDHPHDAWRKALPFLIDVYKL
GKATMTDDIVVFWYRRNFVSACSVAGTTVNTASQLQIEFEPSSALEDRIFVMGLFQTGNS
AVFVNNHDIVVWDSVPNGDLEAGVWFGSVAATTGKVRVDLLKGDATIGSAVGPEISHTCE
GDFNNFNAWVGSVSGGIMSSSDTTSIDLADQACVEGKGAYDFNDLCSFTCSYGYCPVGAC
TCERMGVARKLPNATGTIGYPAEGKDANYQGLCSFACNYGHCPSKTCDTTKYPMPVPTVS
DFLPPACVAGTGPGPVSGLCSYACTYGYCPINLCSCTKTGALVQPPAQTDGAGMAGPGYS
DIFDNLCDFACSRGYCPSGICISKDGVSSANMNYHYDDSAASKCDESQKQVIELELQYAV
EMAQVSALNLQQGDYLDNLFNAKDDSQFISDTTKTFKRIASLLAGSDPEFPLVLSCDRSV
LTELCQQGYVAFMNNKEQRMTFCAPFFKPPDPASTLPQIWPTEDLLEDTQMDLRKASRAR
SAVLVHESTHTRYGMLGKPL

Enzyme Prediction     

EC	3.2.1.59:2	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH71	24	409	3.6e-141	0.992
CBM24	452	527	9.3e-31	0.9868421052631579
CBM24	546	621	1e-26	0.9868421052631579

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397634	Glyco_hydro_71	1.90e-172	24	409	1	372	Glycosyl hydrolase family 71. Family of alpha-1,3-glucanases.
211418	GH71	1.65e-128	18	301	1	283	Glycoside hydrolase family 71. This family of glycoside hydrolases 71 (following the CAZY nomenclature) function as alpha-1,3-glucanases (mutanases, EC 3.2.1.59). They appear to have an endo-hydrolytic mode of enzymatic activity and bacterial members are investigated as candidates for the development of dental caries treatments.The member from fission yeast, endo-alpha-1,3-glucanase Agn1p, plays a vital role in daughter cell separation, while Agn2p has been associated with endolysis of the ascus wall.
211414	GH99_GH71_like	1.38e-49	24	295	1	283	Glycoside hydrolase families 71, 99, and related domains. This superfamily of glycoside hydrolases contains families GH71 and GH99 (following the CAZY nomenclature), as well as other members with undefined function and specificity.
211416	GH99_GH71_like_3	4.61e-11	48	260	104	309	Uncharacterized glycoside hydrolase family 99-like domain. This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism.
199914	M35_deuterolysin_like	1.25e-09	644	792	2	130	Peptidase M35 domain of deuterolysins and related proteins. This family M35 Zn2+-metallopeptidase extracellular domain is found in fungal deutrolysins (acid metalloproteinase, neutral proteinase II), including some well-characterized metallopeptidase domains in Aspergillus oryzae (NpII), Aspergillus fumigatus (MEP20), Penicillium roqueforti (protease II) and Emericella nidulans (PepJ peptidase). The neutral proteinase II from Aspergillus oryzae (NpII) unfolds reversibly upon incubation at higher temperatures, and loss in activity is mainly due to autoproteolysis. MEP20 is encoded by the mepB gene, which appears to be associated with the cytoplasmic degradation of small peptides. PepJ peptidase is a thermostable enzyme released under carbon starvation. Most members of this family contain a unique zinc-binding motif (the aspzincin motif), defined by the HExxH + D motif where an aspartic acid is the third zinc ligand and is found in a GTXDXXYG or similar motif C-terminal to the His zinc ligands. The aspzincin motif is poorly conserved in one subgroup, that includes Asp f2, a major allergen from Aspergillus fumigatus. This subgroup in addition lacks the key conserved Tyr residue which acts as a proton donor during catalysis, and no protease activity has been detected to date for Asp f2.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAP83026.1|CBM24|GH71	0.0	1	800	1	800
BCS05636.1|CBM24|GH71	4.48e-182	29	507	1	475
QYT03219.1|CBM24|GH71	5.30e-182	18	797	19	799
UQC77500.1|CBM24|GH71	4.67e-181	21	554	19	549
QMW33048.1|CBM24|GH71	2.82e-174	22	509	79	566

PDB Hits     

CAP83026:RNA-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|B6GX22|MU71A_PENRW	2.24e-56	5	444	19	439	Mutanase Pc12g07500 OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=PCH_Pc12g07500 PE=1 SV=1
sp|O13716|AGN1_SCHPO	9.09e-48	15	431	14	420	Glucan endo-1,3-alpha-glucosidase agn1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=agn1 PE=1 SV=2
sp|O94510|AGN2_SCHPO	6.33e-30	22	299	11	298	Ascus wall endo-1,3-alpha-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=agn2 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000275	0.999662	CS pos: 21-22. Pr: 0.9710

TMHMM  Annotations     

There is no transmembrane helices in CAP83026:RNA-p1.