CAZyme Information: C4_06000W_A-T-p1

Basic Information

• Species: Candida albicans
• Lineage: Ascomycota; Saccharomycetes; ; Debaryomycetaceae; Candida; Candida albicans
• CAZyme ID: C4_06000W_A-T-p1
• CAZy Family: GT2
• CAZyme Description: Chitobiosyldiphosphodolichol beta-mannosyltransferase [Source:UniProtKB/Swiss-Prot;Acc:Q59Q79]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
456	Ca22chr4A_C_albicans_SC5314|CGC1	52324.75	7.6815

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CalbicansSC5314	6468	237561	256	6212

• Gene Location: location=Ca22chr4A_C_albicans_SC5314:1331712-1333082(+)

Full Sequence      

MGEIIKYKGFDHVWQYSGPWLYCLIGIYISLPVLAYHILPWIFHKNRSNKRKTISIFVLG
DLGHSPRMCYHASSFSKLDYYVNLCGYVETEPSHQIVDDVNIDIIPIEAIKNTNNLPYIM
FAILKVVRQCGKIWSILWDTRGSDYIMIQNPPSIPILLIVILFKTVFSRETKLIIDWHNL
NYTILNLRYNNLNHPFVKLVKLYEKILGQFANLNITVTKSMKKYLVKEFGFQKSKIVTLY
DRPGVQFQPLSNKREFMSEHKLFEDIDIEKYKVLISSTSFTPDEDFNILLDALKNYENTP
NTPPILLIVTGKGPLKGKFLETVDKLEFTNKVCVKSAWLSSEDYPKVLACADLGISLHTS
SSGIDLPMKIVDFFGCGVPVVSLDFPAIDELVKNKVNGLITNSKSDQTKEVARLVTEVFT
DDALLRSIKEGALEESNSRWDENWMQTFSSIFENKS

Enzyme Prediction     

EC	2.4.1.142:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT33	52	449	1.9e-146	0.9905882352941177

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340843	GT33_ALG1-like	0.0	50	452	2	411	chitobiosyldiphosphodolichol beta-mannosyltransferase and similar proteins. This family is most closely related to the GT33 family of glycosyltransferases. The yeast gene ALG1 has been shown to function as a mannosyltransferase that catalyzes the formation of dolichol pyrophosphate (Dol-PP)-GlcNAc2Man from GDP-Man and Dol-PP-Glc-NAc2, and participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. In humans ALG1 has been associated with the congenital disorders of glycosylation (CDG) designated as subtype CDG-Ik.
215155	PLN02275	8.63e-109	50	414	3	370	transferase, transferring glycosyl groups
340839	GT4_GT28_WabH-like	4.05e-16	161	431	96	341	family 4 and family 28 glycosyltransferases similar to Klebsiella WabH. This family is most closely related to the GT1 family of glycosyltransferases. WabH in Klebsiella pneumoniae has been shown to transfer a GlcNAc residue from UDP-GlcNAc onto the acceptor GalUA residue in the cellular outer core.
223515	RfaB	5.11e-16	94	455	35	381	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
340831	GT4_PimA-like	9.26e-16	110	448	54	365	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOW29327.1|GT33	0.0	1	456	1	456
CAX42421.1|GT33	0.0	1	456	1	456
CAR65649.1|GT33	3.86e-211	3	453	5	468
CCE43924.1|GT33	3.78e-207	1	452	1	453
CCG21883.1|GT33	2.05e-205	1	455	1	456

PDB Hits     

C4_06000W_A-T-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q59Q79|ALG1_CANAL	0.0	1	456	1	456	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ALG1 PE=3 SV=1
sp|Q6BS98|ALG1_DEBHA	6.86e-212	3	453	5	468	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=ALG1 PE=3 SV=2
sp|P16661|ALG1_YEAST	1.59e-127	20	451	8	445	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ALG1 PE=1 SV=1
sp|Q6CVU2|ALG1_KLULA	1.76e-123	9	451	5	442	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=ALG1 PE=3 SV=1
sp|Q6FLZ2|ALG1_CANGA	3.88e-123	15	451	5	446	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=ALG1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000061	0.000000

TMHMM  Annotations     

Start	End
20	42