CAZyme Information: Afu8g06360-T-p1

Basic Information

• Species: Aspergillus fumigatus
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus fumigatus
• CAZyme ID: Afu8g06360-T-p1
• CAZy Family: GT69
• CAZyme Description: Putative alpha-1,3-glucanase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
737	Chr8_A_fumigatus_Af293|CGC9	81851.85	4.5965

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AfumigatusAf293	10130	330879	290	9840

• Gene Location: location=Chr8_A_fumigatus_Af293:1524862-1527075(-)

Full Sequence      

MHLQVTNSQNYTLSDWELDMKLAKDAHIDAFAMNMAWEDSTNDHSLEMAFNVANSVGFKL
FFSFDYAGNGPWSQDTVIRMIQQYGSNGAYFQYNGKPFVSTFEGPSNAEDWVTIKAQTGC
FFIPDWSSVGAKPAVALANGVADGLFSWSAWPWGNQTMDTYTDASYIQFLGGKPYMMAIS
PWFYTNLPGYNKNWLWKGDSLWFDRWQELFGLDPMPEFVEIISWNDYGESHYIGPIYEKS
MAAFDIGKSLYNYARDYPHDGWREVLPFLIDLYKNGKASVDHDTVVFWYRPHPVSSCFTG
GTTVNTASQLQIEFEPAFALEDRLYVMALLSDGNHAVRVYAGGDQGYVKWNSRPDEEIVT
GIFFGSVPFHPGKVSIDLDRGDGEAGYAVGLEISDQCEQGFNNYNAWVGSFTASAIPITK
GTTKVALKDQACIRGKGAYDFNDLCSFTCSYGYCPVGACTCEQMGVPRTKPNATGVIGYP
AEGKDANYLGLCSFACNYGHCPSKTCDTQEHPMPIPTVSDFLPPACTEGTGNGNALGLCS
YACGFGYCPINMCKCTKTGALVEPPPQTKGAGMAAPGQSSVLDNLCDFTCSRGYCPPETC
TYKDELAVAHINPTLRWGGEGASACDATKRSIILLEFRFAILMAQTAQENLQSWGYYETF
FSQGVRNRKDFAQHASLVYKRVVSMLDGSEFDLQITCDNTTPQCQKENPDIAYMNAFRRT
VNICDAFLFEYENLRHT

Enzyme Prediction     

EC	3.2.1.59:2	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH71	2	382	5.2e-138	0.9706666666666667
CBM24	431	506	1.7e-30	0.9868421052631579
CBM24	525	600	1.5e-24	0.9868421052631579

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397634	Glyco_hydro_71	1.63e-176	2	384	4	369	Glycosyl hydrolase family 71. Family of alpha-1,3-glucanases.
211418	GH71	7.77e-127	2	276	10	283	Glycoside hydrolase family 71. This family of glycoside hydrolases 71 (following the CAZY nomenclature) function as alpha-1,3-glucanases (mutanases, EC 3.2.1.59). They appear to have an endo-hydrolytic mode of enzymatic activity and bacterial members are investigated as candidates for the development of dental caries treatments.The member from fission yeast, endo-alpha-1,3-glucanase Agn1p, plays a vital role in daughter cell separation, while Agn2p has been associated with endolysis of the ascus wall.
211414	GH99_GH71_like	6.76e-42	16	270	13	283	Glycoside hydrolase families 71, 99, and related domains. This superfamily of glycoside hydrolases contains families GH71 and GH99 (following the CAZY nomenclature), as well as other members with undefined function and specificity.
199914	M35_deuterolysin_like	2.77e-14	625	727	2	102	Peptidase M35 domain of deuterolysins and related proteins. This family M35 Zn2+-metallopeptidase extracellular domain is found in fungal deutrolysins (acid metalloproteinase, neutral proteinase II), including some well-characterized metallopeptidase domains in Aspergillus oryzae (NpII), Aspergillus fumigatus (MEP20), Penicillium roqueforti (protease II) and Emericella nidulans (PepJ peptidase). The neutral proteinase II from Aspergillus oryzae (NpII) unfolds reversibly upon incubation at higher temperatures, and loss in activity is mainly due to autoproteolysis. MEP20 is encoded by the mepB gene, which appears to be associated with the cytoplasmic degradation of small peptides. PepJ peptidase is a thermostable enzyme released under carbon starvation. Most members of this family contain a unique zinc-binding motif (the aspzincin motif), defined by the HExxH + D motif where an aspartic acid is the third zinc ligand and is found in a GTXDXXYG or similar motif C-terminal to the His zinc ligands. The aspzincin motif is poorly conserved in one subgroup, that includes Asp f2, a major allergen from Aspergillus fumigatus. This subgroup in addition lacks the key conserved Tyr residue which acts as a proton donor during catalysis, and no protease activity has been detected to date for Asp f2.
211416	GH99_GH71_like_3	9.18e-08	18	238	72	312	Uncharacterized glycoside hydrolase family 99-like domain. This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAP83026.1|CBM24|GH71	1.14e-286	2	727	27	746
BCS05636.1|CBM24|GH71	4.59e-182	5	486	2	475
QMW33048.1|CBM24|GH71	5.26e-175	2	489	84	567
QMW45081.1|CBM24|GH71	5.26e-175	2	489	84	567
QRD84612.1|CBM24|GH71	5.26e-175	2	489	84	567

PDB Hits     

Afu8g06360-T-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|B6GX22|MU71A_PENRW	1.03e-56	2	410	41	429	Mutanase Pc12g07500 OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=PCH_Pc12g07500 PE=1 SV=1
sp|O13716|AGN1_SCHPO	6.62e-47	2	409	27	420	Glucan endo-1,3-alpha-glucosidase agn1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=agn1 PE=1 SV=2
sp|O94510|AGN2_SCHPO	4.16e-25	2	280	16	304	Ascus wall endo-1,3-alpha-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=agn2 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999662	0.000358

TMHMM  Annotations     

There is no transmembrane helices in Afu8g06360-T-p1.