CAZyme Information: Afu6g04210-T-p1

Basic Information

• Species: Aspergillus fumigatus
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus fumigatus
• CAZyme ID: Afu6g04210-T-p1
• CAZy Family: GH65
• CAZyme Description: Mannosyl-oligosaccharide glucosidase, putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
822	Chr6_A_fumigatus_Af293|CGC14	93071.29	4.7007

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AfumigatusAf293	10130	330879	290	9840

• Gene Location: location=Chr6_A_fumigatus_Af293:943325-946181(-)

Full Sequence      

MHFPNLRFLLAPFLYNVVVSGWQASAADDLSVLATETAKANNDSLLWGPYKSNLYFGVRP
RIANSLSAGLMWAKVDNYATAQASFRHTCEQNEGMAGYGWDEYDIRKGGRQTIHDTGNSL
DLTIDFIKVPGGQHGGSWAARVRGIPREDGSPDQPTSIVFYATLEGLGNLGVATESGPIG
YQGDVKLMGYTTELGDFSIDVTAGPETNEHPEHGHPSYEDKPLDRTLVSSSTIAPENLWQ
VKPIFFAQLKSEVNEMIQRFGQENPPPPAQVFTIKNAPGDGNIHLIQKVFKGAFEFDILF
NSGSSPDPVTSDKLTEVISSASQSFSERFNKVLLPQSPFQTAEYSEFSKAMLSNLVGGIG
FFHGDDIVDRSAAPEYDEENEGFWEETAEARARAQPVHEGPKDLFTCVPSRPFFPRGFLW
DEGFHLIPVIDWDTDLALEIVKSWLNLMDEDGWIAREQILGAEARSKVPPEFTVQYPHYA
NPPTLFMVLEAFLDKLEASKGAYSQNSGGQEALDSLRMTYLQKPELGEAFIRSIYPLLKR
HYFWYRTTQKGDIKSYDREAFSTKEAYRWRGRSVQHILTSGLDDYPRPQPPHPGELHVDL
ISWMGMMTRAMRRIAEFVGETEDVEEFRGYETAIERNIDDLHWDEEAQTYCDATIDEFEE
SVHVCHKGYISLFPFLTGMLGPDSPRLKAVLDLIRDPDELWSDYGIRSLSKKDEFYGTAE
NYWRSPIWMPINYLVVKNLYDIAMTSGPHQEQAREMYSSLRKNLVENVFRQWKETGFAWE
QYNPETGKGQRTQHFTGWTSMVVKIMSMPDLPANKQIGHDEL

Enzyme Prediction     

EC	3.2.1.106:25	3.2.1.-:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH63	595	806	3.8e-35	0.43157894736842106

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397353	Glyco_hydro_63	0.0	310	808	2	494	Glycosyl hydrolase family 63 C-terminal domain. This is a family of eukaryotic enzymes belonging to glycosyl hydrolase family 63. They catalyze the specific cleavage of the non-reducing terminal glucose residue from Glc(3)Man(9)GlcNAc(2). Mannosyl oligosaccharide glucosidase EC:3.2.1.106 is the first enzyme in the N-linked oligosaccharide processing pathway. This family represents the C-terminal catalytic domain.
407154	Glyco_hydro_63N	3.98e-129	45	267	1	221	Glycosyl hydrolase family 63 N-terminal domain. This is a family of eukaryotic enzymes belonging to glycosyl hydrolase family 63. They catalyze the specific cleavage of the non-reducing terminal glucose residue from Glc(3)Man(9)GlcNAc(2). Mannosyl oligosaccharide glucosidase EC:3.2.1.106 is the first enzyme in the N-linked oligosaccharide processing pathway. This family represents the N-terminal beta sandwich domain.
225942	GDB1	6.63e-07	528	756	358	561	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].
236653	PRK10137	8.97e-06	598	800	578	773	alpha-glucosidase; Provisional
215307	PLN02567	2.91e-04	598	819	328	554	alpha,alpha-trehalase

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAR23808.1|GH63	0.0	1	822	1	822
BCS02288.1|GH63	0.0	1	822	1	822
BCS13967.1|GH63	0.0	1	822	1	822
CAL00956.1|GH63	0.0	1	822	1	822
BAK64066.1|GH63|3.2.1.106	0.0	1	822	1	822

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4J5T_A	8.37e-179	36	806	6	800	Crystal structure of Processing alpha-Glucosidase I [Saccharomyces cerevisiae S288C]
5MHF_A	6.95e-140	45	807	37	776	Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus],5MHF_B Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus],5MHF_C Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus],5MHF_D Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O14255|GCS1_SCHPO	1.05e-252	6	807	13	805	Probable mannosyl-oligosaccharide glucosidase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC6G10.09 PE=3 SV=1
sp|P53008|CWH41_YEAST	5.31e-179	24	806	22	830	Mannosyl-oligosaccharide glucosidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CWH41 PE=1 SV=1
sp|Q80UM7|MOGS_MOUSE	4.44e-141	45	807	92	831	Mannosyl-oligosaccharide glucosidase OS=Mus musculus OX=10090 GN=Mogs PE=1 SV=1
sp|O88941|MOGS_RAT	2.08e-133	47	807	94	831	Mannosyl-oligosaccharide glucosidase OS=Rattus norvegicus OX=10116 GN=Mogs PE=1 SV=1
sp|Q13724|MOGS_HUMAN	1.22e-132	34	807	83	834	Mannosyl-oligosaccharide glucosidase OS=Homo sapiens OX=9606 GN=MOGS PE=1 SV=5

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.236540	0.763431	CS pos: 26-27. Pr: 0.6698

TMHMM  Annotations     

There is no transmembrane helices in Afu6g04210-T-p1.